LOCUS       40924_1559        7918 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector L248-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, 
            Klimovich BV, Samoilovich MP, Satou Y, Kruss M, Gelhaus C, Kurn U, 
            Bosch TC, Khalturin K.
  TITLE     Blood System Formation in the Urochordate Ciona intestinalis 
            Requires the Variable Receptor vCRL1
  JOURNAL   Mol. Biol. Evol. 29 (10), 3081-3093 (2012)
  PUBMED    22513285
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, 
            Klimovich VB, Samoilovich MP, Satou Y, Kruess M, Gelhaus C, Kuern U,
            Bosch TCG., Khalturin K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2011) Zoological Institute, Christian-Albrechts 
            University Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 
            24118, Germany
REFERENCE   3  (bases 1 to 7918)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7918)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biol. 
            Evol."; date: "2012"; volume: "29"; issue: "10"; pages: "3081-3093"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-2011) Zoological Institute, Christian-Albrechts University 
            Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 24118, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2531..3244
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(5075..5093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5103..5119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5261..5716
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5742..5846
                     /label=AmpR promoter
     CDS             5847..6704
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6878..7466
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7754..7775
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7790..7820
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7828..7844
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7852..7868
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7889..7907
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtaccaagt gcttgaaatg ctaaatgttt tcaatttttc gccattaaga caagcctaca
       61 caaatgcttc tataaattat gccaagcacg ttagcagctt ctacgagccc caaccactat
      121 taattcgaac agcatgtttt tttgcagtgc gcaatgttta acacactata ttatcaatac
      181 tactaaagat aacacatacc aatgcatttc gtctcaaaga gaattttatt ctcttcacga
      241 cgaaaaaaaa agttttgctc tatttccaac aacaacaaaa atatgagtaa tttattcaaa
      301 cggtttgctt aagagataag aaaaaagtga ccactattaa ttcgaacgcg gcgtaagctt
      361 accttaatct caagaagagc aaaacaaaag caactaatgt aacggaatca ttatctagtt
      421 atgatctgca aataatgctg caggctttgg ttataggcta ttgtttgagg atccgagggg
      481 caactgttta ttaattttga tatttaacat tttatatgcc atgtgtgttt ctgcaagcca
      541 tcttgaagta taatcgagca cggtattagt tttgcgtata aagatataac cagcggggct
      601 gatcgcatat atatagctat agcttacgca cgaataatat ggcgatgtct gactgacgat
      661 cattaaaagc atgtatacaa ccaactacag gttatgggaa atatagtagg gtgggggaag
      721 atgtgacacc tttagcacat aatatccaaa tatcatgata gtgttttaaa gaattaacaa
      781 cggtcaatgg aagtcgtgag gatacggttt aataattcta tgaatgtttt tggttactaa
      841 caaatgagac gataaaatag aatgtaatag tgtctcatct ttccccaacc cactataaat
      901 acagcaaatt gtaagttatg ggaaaaaata caacagtgaa agtataacag tttttgtttc
      961 taacgctata atagcaacag cagaataaac aggtagtacg tatgcatgca ctgattcaag
     1021 actgaaatca aaagaaaaca ttgttgttgt tttggttcga tacttaactc atgttgttta
     1081 agtttttaat cgtgttttga ggaagttccg ctgctttgcg cgagcggcgg aactctggtt
     1141 ccgagaatgt ttgatgtcac gtttccttta gcgaatcttt aaagaaatat caaagttttt
     1201 gtttaacctc ttattcgcga ggacacaatt atgattcgtt taatgagtct gttagttgtg
     1261 cgggacatta ctgggtaaaa ttaatagaaa aatgccaaaa aaagctgaac gattaaattt
     1321 tgagctttct tagaaaaatg agtaaaaatg gcaaatgtag gaagtgctat tatgacgcat
     1381 gttgtagatg atcgaaagcg agtaatgcgt aatatgtctg gcacagctgc aatgttaata
     1441 catcacaatt taattactat acaagagcaa tagttcattt aatatgccac aaagtacgtt
     1501 gccggtgcgc agtgaaaaat atcacagaca atacacgttc ttccgttgac ggacttgaaa
     1561 tacgtgtagc tgataagtga aggaacctga gttgacaaac gaattcttcc ggcaaacaga
     1621 gaccgcattt tcacgatttt ggtttaccgt agcataaata cgtaatattt tgttttggtt
     1681 ttatacaccg acagccggtg tattttgcaa ggattacctg cttcctattt gacaacaaac
     1741 attgcaaatg atggagaata aacaaataat aaatccgacc gaagcaaaaa tattttgcaa
     1801 gcaatctctt ttatatgtgg gtgaggtgcc attgggtggc acgccatggg ttctaggatt
     1861 taagccagaa ttagtggcta taggtacatt aataactgtt ctgacatttc attgtattta
     1921 atgtaatgaa ctatacaaaa ggcacatata tttaaatccg ccaccttaca aaagtaaaaa
     1981 atccgatttc tatattagca ttaatggcta tgaaatagtt tcagttctta acgtcaggta
     2041 attccaaagt attttatttg atttaattag aggatttatt ccttgcctca cagtgaaact
     2101 tattaagcag cttaaacttt tgcggttcgt gcacgaattt cgtggtatga cgtcattata
     2161 tcacaatgta gtggaaaagt taacgttaca ttaaaaaaaa aacgattaat ttgtaaaacg
     2221 taataaggtt atgcagcttg agtttgacga aagcagggct cagttatacg tcatgatacg
     2281 aacgtatatg tctacgaaca ttggcgcgcc ggaattcgct aagtagcaat gtgtatgagt
     2341 aattaggcat cattactcag agccatatta ggggcatcga tagtgcgcag tgaaaatgac
     2401 ggaaaaacgc aacggcgggt atataaggcc tgcgtggatg aggtatatat gacacgttca
     2461 tcgaacgaag ttaaaatatt attttgtgaa gttattaaat atgaagctgc tactattaat
     2521 taatgaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     2581 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     2641 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     2701 gccaacactt gtcactactt tctgttatgg tgttcaatgc ttttcaagat acccagatca
     2761 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     2821 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     2881 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     2941 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     3001 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     3061 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     3121 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     3181 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     3241 caaagcgatc gcgtgactgc agccaacgac accgcccacc atcacttctg cagttttact
     3301 ccaccagctt gctcaccctt ctaaccattt cagcctagtt aaacccccag ttcaatttgt
     3361 cagcctttta aattgcgtca tgacatttca gttgttactg tgtgccgctc tgtccacatg
     3421 gtgtagttat gtttttcatt acccccaccg aaaacacttt cgtaggaaaa aaccctccgt
     3481 ttcgtgccaa cttaaaagtg ttatttttgt ttctagcgct gcttaactta tttaaaatga
     3541 caagactatc tatgatttta aaccatggct ttgaatggcg tacatgtatt attgtgttta
     3601 tattcagtta aaccgtacta ttactgcttg gaaaattcag ttcattcctt caccgaaggc
     3661 tttaacgtgg ttggttgcgg tgttgtcaat ttgctgctgg aaataaagct tctgttaagt
     3721 gtatactgtc tcttggtttg caagattatt atggatccag atgttgtata tacaagagtg
     3781 aagatttagc gtttttatgt ggtaaaacgc ccatggtgat ctatttacag tttttaagtt
     3841 gagctgatca attttcacat gatgatcctg aacgaccagg ggcacaaagg catttgcaga
     3901 ttgtcccatc tctcactacg accgatctac cactattttc acaacggcca gtacatttcg
     3961 aagtctggta tttagcgaag tagtgttcaa ttgccaaagc catgtttctc tgcttcgctc
     4021 tgtctgggat gaccgatgat atgggtcgca gctgtaaaat tacaaatagt cagtttttct
     4081 gacgaaagtg aatgttgatt acattcaaat gaaagtcgtt gatggctaat gccatcgttc
     4141 atacgcttta attagcagat tatgtggtta tattagatat ctttaccagt aatattgaga
     4201 acgccaaggt aaaatctagt tttttgatac ttaatgaaca gccatattaa aagccataca
     4261 tgaaaaaaaa attaaaaaaa acatacataa actctcataa gctgacacag taggtcgtgg
     4321 ctgacacgag gtgtatgaaa caaaacgctc gtgttttaac gactgttgtt gcaccgtcac
     4381 gcgagaataa agtaacttcc attcatacat gatacagttc ttaccttgaa atcgatgact
     4441 gcagggctaa gtttaactga tgctgtccag ttttcgtaag catttgttgt aagtcggttt
     4501 ctagcaaaga aactgagagc actagctgta tgagactgcc caccaatcac attcgatata
     4561 acttccttag cagccattgt aaaacttcct aaatattagt aaataacgaa taataatgtg
     4621 tacatgactc tgcttatcag ttagattggg cgcgccatca gtttaaacga atgcggccgc
     4681 caccgcggga cgttacacaa ttctaatatt aattaaatta ttgttttaag tatgatagta
     4741 aatcacatta cgccgcgttc gaattaatag tggtcacttt tttcttatct cttaagcaaa
     4801 ccgtttgaat aaattactca tatttttgtt gttgttggaa atagagcaaa actttttttt
     4861 tcgtcgtgaa gagaataaaa ttctctttga gacgaaatgc attggtatgt gttatcttta
     4921 gtagtattga taatatagtg tgttaaacat tgcgcactgc aaaaaaaaca tgctgttcga
     4981 attaatagtg gttggggctc gtagaaaacg aaaaatatct taagctagca tagagaatgg
     5041 agcaaaactc aatttgatgc gagctccaat tcgccctata gtgagtcgta ttacgcgcgc
     5101 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5161 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5221 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     5281 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5341 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5401 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5461 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5521 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5581 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5641 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5701 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5761 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5821 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5881 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5941 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6001 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6061 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6121 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6181 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6241 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6301 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6361 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6421 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6481 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6541 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6601 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6661 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6721 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6781 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6841 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6901 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6961 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7021 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7081 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7141 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7201 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7261 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7321 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7381 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7441 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7501 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7561 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7621 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7681 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7741 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7801 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7861 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagct
//
