LOCUS 40924_1559 7918 bp DNA circular SYN 17-DEC-2018 DEFINITION Expression vector L248-1, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7918) AUTHORS Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, Klimovich BV, Samoilovich MP, Satou Y, Kruss M, Gelhaus C, Kurn U, Bosch TC, Khalturin K. TITLE Blood System Formation in the Urochordate Ciona intestinalis Requires the Variable Receptor vCRL1 JOURNAL Mol. Biol. Evol. 29 (10), 3081-3093 (2012) PUBMED 22513285 REFERENCE 2 (bases 1 to 7918) AUTHORS Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, Klimovich VB, Samoilovich MP, Satou Y, Kruess M, Gelhaus C, Kuern U, Bosch TCG., Khalturin K. TITLE Direct Submission JOURNAL Submitted (16-FEB-2011) Zoological Institute, Christian-Albrechts University Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 24118, Germany REFERENCE 3 (bases 1 to 7918) TITLE Direct Submission REFERENCE 4 (bases 1 to 7918) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biol. Evol."; date: "2012"; volume: "29"; issue: "10"; pages: "3081-3093" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (16-FEB-2011) Zoological Institute, Christian-Albrechts University Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 24118, Germany" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7918 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 2531..3244 /codon_start=1 /label=GFP /note="Aequorea victoria green fluorescent protein" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" promoter complement(5075..5093) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5103..5119) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 5261..5716 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 5742..5846 /label=AmpR promoter CDS 5847..6704 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6878..7466 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 7754..7775 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 7790..7820 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7828..7844 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7852..7868 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 7889..7907 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 ggtaccaagt gcttgaaatg ctaaatgttt tcaatttttc gccattaaga caagcctaca 61 caaatgcttc tataaattat gccaagcacg ttagcagctt ctacgagccc caaccactat 121 taattcgaac agcatgtttt tttgcagtgc gcaatgttta acacactata ttatcaatac 181 tactaaagat aacacatacc aatgcatttc gtctcaaaga gaattttatt ctcttcacga 241 cgaaaaaaaa agttttgctc tatttccaac aacaacaaaa atatgagtaa tttattcaaa 301 cggtttgctt aagagataag aaaaaagtga ccactattaa ttcgaacgcg gcgtaagctt 361 accttaatct caagaagagc aaaacaaaag caactaatgt aacggaatca ttatctagtt 421 atgatctgca aataatgctg caggctttgg ttataggcta ttgtttgagg atccgagggg 481 caactgttta ttaattttga tatttaacat tttatatgcc atgtgtgttt ctgcaagcca 541 tcttgaagta taatcgagca cggtattagt tttgcgtata aagatataac cagcggggct 601 gatcgcatat atatagctat agcttacgca cgaataatat ggcgatgtct gactgacgat 661 cattaaaagc atgtatacaa ccaactacag gttatgggaa atatagtagg gtgggggaag 721 atgtgacacc tttagcacat aatatccaaa tatcatgata gtgttttaaa gaattaacaa 781 cggtcaatgg aagtcgtgag gatacggttt aataattcta tgaatgtttt tggttactaa 841 caaatgagac gataaaatag aatgtaatag tgtctcatct ttccccaacc cactataaat 901 acagcaaatt gtaagttatg ggaaaaaata caacagtgaa agtataacag tttttgtttc 961 taacgctata atagcaacag cagaataaac aggtagtacg tatgcatgca ctgattcaag 1021 actgaaatca aaagaaaaca ttgttgttgt tttggttcga tacttaactc atgttgttta 1081 agtttttaat cgtgttttga ggaagttccg ctgctttgcg cgagcggcgg aactctggtt 1141 ccgagaatgt ttgatgtcac gtttccttta gcgaatcttt aaagaaatat caaagttttt 1201 gtttaacctc ttattcgcga ggacacaatt atgattcgtt taatgagtct gttagttgtg 1261 cgggacatta ctgggtaaaa ttaatagaaa aatgccaaaa aaagctgaac gattaaattt 1321 tgagctttct tagaaaaatg agtaaaaatg gcaaatgtag gaagtgctat tatgacgcat 1381 gttgtagatg atcgaaagcg agtaatgcgt aatatgtctg gcacagctgc aatgttaata 1441 catcacaatt taattactat acaagagcaa tagttcattt aatatgccac aaagtacgtt 1501 gccggtgcgc agtgaaaaat atcacagaca atacacgttc ttccgttgac ggacttgaaa 1561 tacgtgtagc tgataagtga aggaacctga gttgacaaac gaattcttcc ggcaaacaga 1621 gaccgcattt tcacgatttt ggtttaccgt agcataaata cgtaatattt tgttttggtt 1681 ttatacaccg acagccggtg tattttgcaa ggattacctg cttcctattt gacaacaaac 1741 attgcaaatg atggagaata aacaaataat aaatccgacc gaagcaaaaa tattttgcaa 1801 gcaatctctt ttatatgtgg gtgaggtgcc attgggtggc acgccatggg ttctaggatt 1861 taagccagaa ttagtggcta taggtacatt aataactgtt ctgacatttc attgtattta 1921 atgtaatgaa ctatacaaaa ggcacatata tttaaatccg ccaccttaca aaagtaaaaa 1981 atccgatttc tatattagca ttaatggcta tgaaatagtt tcagttctta acgtcaggta 2041 attccaaagt attttatttg atttaattag aggatttatt ccttgcctca cagtgaaact 2101 tattaagcag cttaaacttt tgcggttcgt gcacgaattt cgtggtatga cgtcattata 2161 tcacaatgta gtggaaaagt taacgttaca ttaaaaaaaa aacgattaat ttgtaaaacg 2221 taataaggtt atgcagcttg agtttgacga aagcagggct cagttatacg tcatgatacg 2281 aacgtatatg tctacgaaca ttggcgcgcc ggaattcgct aagtagcaat gtgtatgagt 2341 aattaggcat cattactcag agccatatta ggggcatcga tagtgcgcag tgaaaatgac 2401 ggaaaaacgc aacggcgggt atataaggcc tgcgtggatg aggtatatat gacacgttca 2461 tcgaacgaag ttaaaatatt attttgtgaa gttattaaat atgaagctgc tactattaat 2521 taatgaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga 2581 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc 2641 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg 2701 gccaacactt gtcactactt tctgttatgg tgttcaatgc ttttcaagat acccagatca 2761 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac 2821 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga 2881 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct 2941 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca 3001 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca 3061 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga 3121 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca 3181 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata 3241 caaagcgatc gcgtgactgc agccaacgac accgcccacc atcacttctg cagttttact 3301 ccaccagctt gctcaccctt ctaaccattt cagcctagtt aaacccccag ttcaatttgt 3361 cagcctttta aattgcgtca tgacatttca gttgttactg tgtgccgctc tgtccacatg 3421 gtgtagttat gtttttcatt acccccaccg aaaacacttt cgtaggaaaa aaccctccgt 3481 ttcgtgccaa cttaaaagtg ttatttttgt ttctagcgct gcttaactta tttaaaatga 3541 caagactatc tatgatttta aaccatggct ttgaatggcg tacatgtatt attgtgttta 3601 tattcagtta aaccgtacta ttactgcttg gaaaattcag ttcattcctt caccgaaggc 3661 tttaacgtgg ttggttgcgg tgttgtcaat ttgctgctgg aaataaagct tctgttaagt 3721 gtatactgtc tcttggtttg caagattatt atggatccag atgttgtata tacaagagtg 3781 aagatttagc gtttttatgt ggtaaaacgc ccatggtgat ctatttacag tttttaagtt 3841 gagctgatca attttcacat gatgatcctg aacgaccagg ggcacaaagg catttgcaga 3901 ttgtcccatc tctcactacg accgatctac cactattttc acaacggcca gtacatttcg 3961 aagtctggta tttagcgaag tagtgttcaa ttgccaaagc catgtttctc tgcttcgctc 4021 tgtctgggat gaccgatgat atgggtcgca gctgtaaaat tacaaatagt cagtttttct 4081 gacgaaagtg aatgttgatt acattcaaat gaaagtcgtt gatggctaat gccatcgttc 4141 atacgcttta attagcagat tatgtggtta tattagatat ctttaccagt aatattgaga 4201 acgccaaggt aaaatctagt tttttgatac ttaatgaaca gccatattaa aagccataca 4261 tgaaaaaaaa attaaaaaaa acatacataa actctcataa gctgacacag taggtcgtgg 4321 ctgacacgag gtgtatgaaa caaaacgctc gtgttttaac gactgttgtt gcaccgtcac 4381 gcgagaataa agtaacttcc attcatacat gatacagttc ttaccttgaa atcgatgact 4441 gcagggctaa gtttaactga tgctgtccag ttttcgtaag catttgttgt aagtcggttt 4501 ctagcaaaga aactgagagc actagctgta tgagactgcc caccaatcac attcgatata 4561 acttccttag cagccattgt aaaacttcct aaatattagt aaataacgaa taataatgtg 4621 tacatgactc tgcttatcag ttagattggg cgcgccatca gtttaaacga atgcggccgc 4681 caccgcggga cgttacacaa ttctaatatt aattaaatta ttgttttaag tatgatagta 4741 aatcacatta cgccgcgttc gaattaatag tggtcacttt tttcttatct cttaagcaaa 4801 ccgtttgaat aaattactca tatttttgtt gttgttggaa atagagcaaa actttttttt 4861 tcgtcgtgaa gagaataaaa ttctctttga gacgaaatgc attggtatgt gttatcttta 4921 gtagtattga taatatagtg tgttaaacat tgcgcactgc aaaaaaaaca tgctgttcga 4981 attaatagtg gttggggctc gtagaaaacg aaaaatatct taagctagca tagagaatgg 5041 agcaaaactc aatttgatgc gagctccaat tcgccctata gtgagtcgta ttacgcgcgc 5101 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 5161 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 5221 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca 5281 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 5341 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 5401 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 5461 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 5521 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 5581 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 5641 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 5701 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 5761 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 5821 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 5881 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 5941 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 6001 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 6061 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 6121 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 6181 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 6241 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 6301 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 6361 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 6421 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 6481 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 6541 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 6601 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 6661 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 6721 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 6781 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 6841 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 6901 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 6961 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 7021 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 7081 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 7141 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 7201 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 7261 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 7321 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 7381 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 7441 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 7501 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 7561 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 7621 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 7681 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 7741 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 7801 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 7861 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagct //