LOCUS       40924_1529        9502 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Retroviral expression vector L149, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Micklem DR, Lorens JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2008) Department of Biomedicine, University of 
            Bergen, Jonas Lies vei 91, Bergen 5009, Norway
REFERENCE   2  (bases 1 to 9502)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9502)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (27-MAY-2008) Department of Biomedicine, University of Bergen, Jonas
            Lies vei 91, Bergen 5009, Norway"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        62..365
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        366..569
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    601..609
                     /label=R region 5'LTR
                     /note="R region 5'LTR"
     misc_feature    670..745
                     /label=U5 region 5'LTR
                     /note="U5 region 5'LTR"
     misc_feature    808..1165
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1230..1646
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1656..2030
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     polyA_signal    complement(2049..2256)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    complement(2946..3029)
                     /gene="P2AG2ALuc2ANTR"
                     /label=FMDV 2A self-cleaving linker region
                     /note="FMDV 2A self-cleaving linker region"
     CDS             complement(3030..4679)
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    complement(4680..4763)
                     /gene="P2AG2ALuc2ANTR"
                     /label=FMDV 2A self-cleaving linker region
                     /note="FMDV 2A self-cleaving linker region"
     CDS             complement(4764..5480)
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    5481..5549
                     /label=FMDV 2A self-cleaving linker region
                     /note="FMDV 2A self-cleaving linker region"
     CDS             complement(5553..6146)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPG"
     intron          complement(6230..6362)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      complement(6465..6517)
                     /label=PhCMV* minimal promoter
                     /note="PhCMV* minimal promoter"
                     /regulatory_class="promoter"
     promoter        complement(6480..6517)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    complement(6550..6820)
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     misc_feature    6892..7056
                     /label=U3SIN (self-inactivating) region
                     /note="U3SIN (self-inactivating) region"
     repeat_region   6892..7056
                     /label=MoMLV 3'LTR, self-inactivating
                     /note="MoMLV 3'LTR, self-inactivating"
     misc_feature    7058..7125
                     /label=R region 3'LTR
                     /note="R region 3'LTR"
     primer_bind     complement(7278..7294)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7302..7318)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7326..7356)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7371..7392)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7680..8268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8445..9302)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9303..9407)
                     /label=AmpR promoter
ORIGIN
        1 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
       61 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      121 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      181 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      241 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      301 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      361 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      421 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      481 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcatg
      541 tacggtggga ggtctatata agcagagctc aataaaagag cccacaaccc ctcactcggc
      601 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
      661 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
      721 tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca
      781 gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga
      841 ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct
      901 ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg
      961 gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg
     1021 atcgttttgg actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag
     1081 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa
     1141 gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     1201 ttctgtattt gtctgaaaat atgggcccgg gccagactgt taccactccc ttaagtttga
     1261 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     1321 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     1381 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     1441 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     1501 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     1561 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     1621 cagccctcac tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac
     1681 ccccgcccct tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc
     1741 tccaagctca cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg
     1801 cagcctacca agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca
     1861 cagtgtgggt ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca
     1921 cagtcctgct gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg
     1981 ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctcg
     2041 aaggatcctc cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc
     2101 ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat
     2161 tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg
     2221 ggcaaacaac agatggctgg caactagaag gcacagtcga ggtctaggcg gccgcccggg
     2281 tcgactctag attacacttc ggttaaggtg atgttttgcg gcagacgaga tttcggcagc
     2341 gtagcgttaa aatcttcaac gctgtgatga cctaccggaa caaccaccag actggtgtag
     2401 cctttctctt tcagaccaaa ttctgcatcg aggatggcgg cgtcaaaacc ttcgatgggt
     2461 accgcgtcca gacccagagc cgccacgccg agcaggaagt taccgacgtt gagataaacc
     2521 tgttttgcca tccactctgc atcatcatgc agatccttac ggtgcatatc agcgaagaac
     2581 ttgcgacctt tatcgttcgc ggctttcgct tccggcgtgg caaagcggcc atcggcatct
     2641 tcctggtcaa caaccagctt cagccagaca tcgtccatcg cggtttttgc acagaacacc
     2701 acgacgtgcg aggcatcaag cattttacgc tcgttgaaca cgtaattacc ggcagcggat
     2761 ttggcaacac gcgctttacc ttcttccgtg ctggcaacaa taaaatgcca cggctgggag
     2821 ttggtgctgg atgggctgta ttgcagtagc gttttgatct gctcggcctg ttccggggta
     2881 agttttttgc tggcatcaaa tgccttagtg gaatgacgct ttaaggcgac agaaatgata
     2941 tccatgggcc cagggttgga ctcgacgtct cccgcaagtt taagaaggtc aaaattcaac
     3001 agctgtccgg atctgagtcc ggagaattcc acggcgatct ttccgccctt cttggccttt
     3061 atgaggatct ctctgatttt tcttgcgtcg agttttccgg taagaccttt cggtacttcg
     3121 tccacaaaca caactcctcc gcgcaacttt ttcgcggttg ttacttgact ggcgacgtaa
     3181 tccacgatct ctttttccgt catcgtcttt ccgtgctcca aaacaacaac ggcggcggga
     3241 agttcaccgg cgtcatcgtc gggaagacct gcgacacctg cgtcgaagat gttggggtgt
     3301 tggagcaaga tggattccaa ttcagcggga gccacctgat agcctttgta cttaatcaga
     3361 gacttcaggc ggtcaacgat gaagaagtgt tcgtcttcgt cccagtaagc tatgtctcca
     3421 gaatgtagcc atccatcctt gtcaatcaag gcgttggtcg cttccggatt gtttacataa
     3481 ccggacataa tcataggacc tctcacacac agttcgcctc tttgattaac gcccagcgtt
     3541 ttcccggtat ccagatccac aaccttcgct tcaaaaaatg gaacaacttt accgaccgcg
     3601 cccggtttat catccccctc gggtgtaatc agaatagctg atgtagtctc agtgagccca
     3661 tatccttgcc tgatacctgg cagatggaac ctcttggcaa ccgcttcccc gacttcctta
     3721 gagaggggag cgccaccaga agcaatttcg tgtaaattag ataaatcgta tttgtcaatc
     3781 agagtgcttt tggcgaagaa ggagaatagg gttggcacca gcagcgcact ttgaatcttg
     3841 taatcctgaa ggctcctcag aaacagctct tcttcaaatc tatacattaa gacgactcga
     3901 aatccacata tcaaatatcc gagtgtagta aacattccaa aaccgtgatg gaatggaaca
     3961 acacttaaaa tcgcagtatc cggaatgatt tgattgccaa aaataggatc tctggcatgc
     4021 gagaatctca cgcaggcagt tctatgaggc agagcgacac ctttaggcag accagtagat
     4081 ccagaggagt tcatgatcag tgcaattgtc ttgtccctat cgaaggactc tggcacaaaa
     4141 tcgtattcat taaaaccggg aggtagatga gatgtgacga acgtgtacat cgactgaaat
     4201 ccctggtaat ccgttttaga atccatgata ataatttttt ggatgattgg gagctttttt
     4261 tgcacgttca aaattttttg caaccccttt ttggaaacga acaccacggt aggctgcgaa
     4321 atgcccatac tgttgagcaa ttcacgttca ttataaatgt cgttcgcggg cgcaactgca
     4381 actccgataa ataacgcgcc caacaccggc ataaagaatt gaagagagtt ttcactgcat
     4441 acgacgattc tgtgatttgt attcagccca tatcgtttca tagcttctgc caaccgaacg
     4501 gacatttcga agtactcagc gtaagtgatg tccacctcga tatgtgcatc tgtaaaagca
     4561 attgttccag gaaccagggc gtatctcttc atagccttat gcagttgctc tccagcggtt
     4621 ccatcttcca gcggatagaa tggcgccggg cctttcttta tgtttttggc gtcttccatg
     4681 ggcccagggt tggactcgac gtctcccgca agtttaagaa ggtcaaaatt caacagctgt
     4741 ccggatctga gtccggagaa ttccttgtac agctcgtcca tgccgagagt gatcccggcg
     4801 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
     4861 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
     4921 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
     4981 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
     5041 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
     5101 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     5161 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
     5221 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
     5281 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
     5341 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
     5401 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
     5461 tcctcgccct tgctcaccat gggcccaggg ttggactcga cgtctcccgc aagtttaaga
     5521 aggtcaaaat tcaacagctg tccggatcca gcaccaggct tgcgggtcat gcaccaggtg
     5581 cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag
     5641 gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc
     5701 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg
     5761 ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
     5821 atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg accgatctcg
     5881 gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg
     5941 tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc
     6001 agctcggtga cccgctcgat gtggcggtcc gggtcgacgg tgtggcgcgt ggcggggtag
     6061 tcggcgaacg cggcggcgag ggtgcgtacg gcccggggga cgtcgtcgcg ggtggcgagg
     6121 cgcaccgtgg gcttgtactc ggtcatggtg gctcgaggac agcaagtctc tagcgaattc
     6181 ggccagatcg gcctaggtta agagctgtat tgaacctggg agtggacacc tgtggagaga
     6241 aaggcaaagt ggatgtcagt aagaccaata ggtgcctatc agaaacgcaa gagtcttctc
     6301 tgtctcgaca agcccagttt ctattggtct ccttaaacct gtcttgtaac cttgatactt
     6361 acctgcccag tgcctcacga ccaacttctg cagcggaggc tggatcggtc ccggtgtctt
     6421 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
     6481 gctctgctta tataggcctc ccaccgtaca cgcctactcg acccgggtac cgagctcgac
     6541 tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca
     6601 ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa
     6661 ctcgactttc acttttctct atcactgata gggagtggta aactcgactt tcacttttct
     6721 ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt
     6781 ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact cgatcgagag
     6841 gccgctcgac gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     6901 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     6961 atacataact gagaatagaa aagttcagat caaggtcagg aacagatgga acagggtcgc
     7021 gtcccgcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
     7081 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     7141 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     7201 catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc
     7261 atagtttcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7321 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7381 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7441 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7501 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7561 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7621 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7681 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7741 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7801 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7861 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7921 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7981 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8041 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8101 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8161 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8221 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8281 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8341 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagtttgcgc
     8401 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8461 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8521 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8581 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8641 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8701 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8761 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8821 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8881 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8941 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9001 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
     9061 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9121 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9181 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9241 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9301 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9361 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9421 aaagtgccac cagctttgct cttaggagtt tcctaataca tcccaaactc aaatatataa
     9481 agcatttgac ttgttctatg cc
//
