LOCUS       V015849                 8403 bp    DNA     circular SYN 29-JUL-2019
DEFINITION  Exported.
ACCESSION   V015849
VERSION     V015849
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Castillo-Hair S, Baerman E, Fujita M, Igoshin O, Tabor J.
  TITLE     Optogenetic control of Bacillus subtilis gene expression
  JOURNAL   Nat Commun 10 (1), 3099 (2019)
   PUBMED   31308373
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Castillo-Hair SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAY-2019) Bioengineering, Rice University, 6100 Main
            St., Houston, TX 77005, USA
REFERENCE   3  (bases 1 to 8403)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "3099"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-MAY-2019) Bioengineering, Rice University, 6100 Main St.,
            Houston, TX 77005, USA"
FEATURES             Location/Qualifiers
     source          1..8403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            115..634
                     /pseudo=""
                     /gene="amyE"
                     /label="amyE"
                     /note="alpha-amylase"
     CDS             complement(647..1360)
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     regulatory      complement(1361..1384)
                     /label="MF001"
                     /note="MF001"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(1385..1458)
                     /label="PrpsD"
                     /note="PrpsD"
                     /regulatory_class="promoter"
     regulatory      1400
                     /label="+1"
                     /note="+1"
                     /regulatory_class="other"
     regulatory      complement(1408..1413)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(1431..1436)
                     /regulatory_class="minus_35_signal"
     gene            1765..2517
                     /gene="spc"
                     /label="spc"
     CDS             1765..2517
                     /codon_start=1
                     /transl_table=11
                     /gene="spc"
                     /product="spectinomycin resistance protein"
                     /label="spc"
                     /protein_id="QDQ17183.1"
                     /translation="MNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNSDLDFL
                     VVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQEFIYG
                     EWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVRRAIMD
                     SSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERILLAVR
                     SYLGENIEWTNENVNLTINYLNNRLKKL"
     misc_feature    2644..3833
                     /label="homology to PY79"
                     /note="homology to PY79"
     gene            2644..3669
                     /pseudo=""
                     /gene="amyE"
                     /label="amyE"
                     /note="alpha-amylase"
     CDS             5032..5763
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     rep_origin      complement(6574..7162)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7336..8193)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8194..8298)
                     /label="AmpR promoter"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcggatcc tcatcatttg tacagttcat
      661 ccataccatg cgtgatgccc gctgcggtta cgaactccag cagaaccata tgatcgcgtt
      721 tctcgttcgg atctttagac agaacgcttt gcgtgctcag atagtgattg tctggcagca
      781 gaacaggacc atcaccgatt ggagtgtttt gctggtagtg atcagccagc tgcacgctgc
      841 catcctccac gttgtggcga attttaaaat tcgctttaat gccatttttt tgtttatcgg
      901 cggtgatgta aacattgtgg ctgttaaaat tgtattccag cttatggccc aggatattgc
      961 cgtcttcttt aaagtcaatg cctttcagct caatgcggtt taccagggta tcgccttcaa
     1021 atttcacttc cgcacgcgtt ttgtacgtgc cgtcatcctt aaaggaaatc gtgcgttcct
     1081 gcacatagcc ttccggcatg gcggacttga agaagtcatg ctgcttcata tggtccggat
     1141 aacgagcaaa gcactgaaca ccataagtca gcgtcgttac cagagtcggc caaggtaccg
     1201 gcagtttacc agtagtacag atgaacttca gcgtcagttt accattagtt gcgtcacctt
     1261 caccctcgcc acgcacggaa aacttatgac cgttgacatc accatccagt tccaccagaa
     1321 tagggacgac accagtgaac agctcttcgc ctttacgcat agtagttcct ccttatgtaa
     1381 gcttctgcaa tatttcacac aaaggtcatt atataattta tttgttttga agtcaaccat
     1441 tcctccatta ttgcttcaga attcctgcag ctggcgaatg gcgattttcg ttcgtgaata
     1501 catgttataa taactataac taataacgta acgtgactgg caagagatat ttttaaaaca
     1561 atgaataggt ttacacttac tttagtttta tggaaatgaa agatcatatc atatataatc
     1621 tagaataaaa ttaactaaaa taattattat ctagataaaa aatttagaag ccaatgaaat
     1681 ctataaataa actaaattaa gtttatttaa ttaacaacta tggatataaa ataggtacta
     1741 atcaaaatag tgaggaggat atatttgaat acatacgaac aaattaataa agtgaaaaaa
     1801 atacttcgga aacatttaaa aaataacctt attggtactt acatgtttgg atcaggagtt
     1861 gagagtggac taaaaccaaa tagtgatctt gactttttag tcgtcgtatc tgaaccattg
     1921 acagatcaaa gtaaagaaat acttatacaa aaaattagac ctatttcaaa aaaaatagga
     1981 gataaaagca acttacgata tattgaatta acaattatta ttcagcaaga aatggtaccg
     2041 tggaatcatc ctcccaaaca agaatttatt tatggagaat ggttacaaga gctttatgaa
     2101 caaggataca ttcctcagaa ggaattaaat tcagatttaa ccataatgct ttaccaagca
     2161 aaacgaaaaa ataaaagaat atacggaaat tatgacttag aggaattact acctgatatt
     2221 ccattttctg atgtgagaag agccattatg gattcgtcag aggaattaat agataattat
     2281 caggatgatg aaaccaactc tatattaact ttatgccgta tgattttaac tatggacacg
     2341 ggtaaaatca taccaaaaga tattgcggga aatgcagtgg ctgaatcttc tccattagaa
     2401 catagggaga gaattttgtt agcagttcgt agttatcttg gagagaatat tgaatggact
     2461 aatgaaaatg taaatttaac tataaactat ttaaataaca gattaaaaaa attataaaaa
     2521 aattgaaaaa atggtggaaa cacttttttc aatttttttg ttttattatt taatatttgg
     2581 gaaatattca ttctaattgg taatcagatt ttagaaaaca ataaaccctt gcataggggg
     2641 atctcgacat ggatgagcga tgatgatatc cgtttaggct gggcggtgat agcttctcgt
     2701 tcaggcagta cgcctctttt cttttccaga cctgagggag gcggaaatgg tgtgaggttc
     2761 ccggggaaaa gccaaatagg cgatcgcggg agtgctttat ttgaagatca ggctatcact
     2821 gcggtcaata gatttcacaa tgtgatggct ggacagcctg aggaactctc gaacccgaat
     2881 ggaaacaacc agatatttat gaatcagcgc ggctcacatg gcgttgtgct ggcaaatgca
     2941 ggttcatcct ctgtctctat caatacggca acaaaattgc ctgatggcag gtatgacaat
     3001 aaagctggag cgggttcatt tcaagtgaac gatggtaaac tgacaggcac gatcaatgcc
     3061 aggtctgtag ctgtgcttta tcctgatgat attgcaaaag cgcctcatgt tttccttgag
     3121 aattacaaaa caggtgtaac acattctttc aatgatcaac tgacgattac cttgcgtgca
     3181 gatgcgaata caacaaaagc cgtttatcaa atcaataatg gaccagagac ggcgtttaag
     3241 gatggagatc aattcacaat cggaaaagga gatccatttg gcaaaacata caccatcatg
     3301 ttaaaaggaa cgaacagtga tggtgtaacg aggaccgaga aatacagttt tgttaaaaga
     3361 gatccagcgt cggccaaaac catcggctat caaaatccga atcattggag ccaggtaaat
     3421 gcttatatct ataaacatga tgggagccga gtaattgaat tgaccggatc ttggcctgga
     3481 aaaccaatga ctaaaaatgc agacggaatt tacacgctga cgctgcctgc ggacacggat
     3541 acaaccaacg caaaagtgat ttttaataat ggcagcgccc aagtgcccgg tcagaatcag
     3601 cctggctttg attacgtgct aaatggttta tataatgact cgggcttaag cggttctctt
     3661 ccccattgag ggcaaggcta gacgggactt accgaaagaa accatcaatg atggtttctt
     3721 ttttgttcat aaatcagaca aaacttttct cttgcaaaag tttgtgaagt gttgcacaat
     3781 ataaatgtga aatacttcac aaacaaaaag acatcaaaga gaaacatacc ctgcaaggat
     3841 gctgatattg tctgcatttg cgccggagca aaccaaaaac ctggtgagac acgccttgaa
     3901 ttagtagaaa agaacttgaa gattttcaaa ggcatcgtta gtgaagtcat ggcgagcgga
     3961 tttgacggca ttttcttagt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     4021 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     4081 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
     4141 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
     4201 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
     4261 tggagccggg ccacctactg aagtggattt ctttaagagc tcctttaact tcctcaccag
     4321 tagttgtatc ggtaccataa gtagaagcag caacccaagt agctttacca gcatccggtt
     4381 caaccagcat agtaagaatc ttactggaca tcggcagttc ttcgaacagt gcgccaacta
     4441 ccagctcttt ctgcagttca ttcagggcac cggagaacct gcgtgcaatc catcttgttc
     4501 aatcatgcga aacgatcctc atcctgtctc ttgatccatg gattacgcgt taacccgggc
     4561 ccgcggatgc atatgatcag atcctttaac tctggcaacc ctcaaaattg aatgagacat
     4621 gctacacctc cggataataa atatatataa acgtatatag atttcataaa gtctaacaca
     4681 ctagacttat ttacttcgta attaagtcgt taaaccgtgt gctctacgac caaaactata
     4741 aaacctttaa gaactttctt tttttacaag aaaaaagaaa ttagataaat ctctcatatc
     4801 ttttattcaa taatcgcatc cgattgcagt ataaatttaa cgatcactca tcatgttcat
     4861 atttatcaga gctcgtgcta taattatact aattttataa ggaggaaaaa atatgggcat
     4921 ttttagtatt tttgtaatca gcacagttca ttatcaacca aacaaaaaat aagtggttat
     4981 aatgaatcgt taataagcaa aattcatata accaaattaa agagggttat aatgaacgag
     5041 aaaaatataa aacacagtca aaactttatt acttcaaaac ataatataga taaaataatg
     5101 acaaatataa gattaaatga acatgataat atctttgaaa tcggctcagg aaaaggccat
     5161 tttacccttg aattagtaaa gaggtgtaat ttcgtaactg ccattgaaat agaccataaa
     5221 ttatgcaaaa ctacagaaaa taaacttgtt gatcacgata atttccaagt tttaaacaag
     5281 gatatattgc agtttaaatt tcctaaaaac caatcctata aaatatatgg taatatacct
     5341 tataacataa gtacggatat aatacgcaaa attgtttttg atagtatagc taatgagatt
     5401 tatttaatcg tggaatacgg gtttgctaaa agattattaa atacaaaacg ctcattggca
     5461 ttacttttaa tggcagaagt tgatatttct atattaagta tggttccaag agaatatttt
     5521 catcctaaac ctaaagtgaa tagctcactt atcagattaa gtagaaaaaa atcaagaata
     5581 tcacacaaag ataaacaaaa gtataattat ttcgttatga aatgggttaa caaagaatac
     5641 aagaaaatat ttacaaaaaa tcaatttaac aattccttaa aacatgcagg aattgacgat
     5701 ttaaacaata ttagctttga acaattctta tctcttttca atagctataa attatttaat
     5761 aagtaagtta agggatgcat aaactgcatc ccttaacttg tttttcgtgt gcctattttt
     5821 tgtgaatcga ttatgtcttt tgcgcagtcg gcttaaacca gttttcgctg gtgcgaaaaa
     5881 agagtgtctt gtgacaccta aattcaaaat ctatcggtca gatttatacc gatttgattt
     5941 tatatattct tgaataacat acgccgagtt atcacataaa agcgggaacc aatcatcaaa
     6001 tttaaacttc attgcataat ccattaaact cttaaattct acgattcctt gttcatcaat
     6061 aaactcaatc atttctttaa ttaatttata tctatctgtt gttgttttct ttaataattc
     6121 atcaacatct acaccgccat aaactatcat atcttctttt tgatatttaa atttattagg
     6181 atcttaaggc ctaggtctag agtctttgtt ttgacgccat tagcgtacgt aacaatcctc
     6241 gttaaaggac aaggacctga gcggaagtgt atcgtacagt agacggagta tactagtata
     6301 gtctatagtc cgtggaatta ttatatttat ctccgacgat attctcatca gtgaaatcca
     6361 gctggagttc tttagcaaat ttttttatta gctgaactta gtattagtgg ccatactcct
     6421 ccaatccaaa gctatttaga aagattacta tatcctcaaa caggcggtaa ccggcctctt
     6481 catcgggaat gcgcgcgacc ttcagcatcg ccggcatgtc cccctggcgg acgggaagta
     6541 tccagctcga ggtcgggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg atagctcttg
     7081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7381 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7621 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     7681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7741 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7921 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     7981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8221 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8281 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     8341 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     8401 att
//
