LOCUS       62056_1286        5161 bp DNA     circular SYN 23-JAN-2019
DEFINITION  Expression vector Hym-176D_EGFP DNA, complete sequence.
ACCESSION   LC426375
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Noro Y, Yum S, Nishimiya-Fujisawa C, Busse C, Shimizu H, Mineta K, 
            Zhang X, Holstein TW, David CN, Gojobori T, Fujisawa T.
  TITLE     Regionalized nervous system in Hydra and the mechanism of its 
            development
  JOURNAL   Gene Expr. Patterns (2019) In press
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Noro Y, Fujisawa T, Shimizu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2018) Contact:Yukihiko Noro Waseda University, 
            Center for Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho, 
            Shinjuku-ku, Tokyo 162-8480, Japan
REFERENCE   3  (bases 1 to 5161)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene Expr. 
            Patterns (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2018) Contact:Yukihiko Noro Waseda University, Center for 
            Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho, 
            Shinjuku-ku, Tokyo 162-8480, Japan"
FEATURES             Location/Qualifiers
     source          1..5161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        362..466
                     /label=AmpR promoter
     CDS             467..1324
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1498..2086
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2374..2395
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2410..2440
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2448..2464
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2472..2488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3882..4595
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
       61 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      121 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
      181 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
      241 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
      301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      361 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      421 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      481 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      541 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      601 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      661 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      721 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      781 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      841 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
      901 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      961 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1021 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1081 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1141 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1201 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1261 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1321 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1381 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1441 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1501 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1561 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1621 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1681 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1741 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1801 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1861 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     1921 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1981 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2041 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2101 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2161 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2221 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2281 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2341 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2401 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2461 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat gctcccggcc
     2521 gccatggcgg ccgcgggaat tcgattaacg gccgcaatgt tgtgcaaaca ggcctgaaga
     2581 aaagtgattt taaaaattaa taataaaata ttttttaatt ttatttagaa atactttgct
     2641 tttttgttta taaacgattg tatcatatta tatcaaaaaa atcaaaatca cataacatta
     2701 gtcaaggtaa cacaacaatc ctgctaaaat aaaggttttt tggcaaatct ttttaggctt
     2761 ttgatggaaa atgaatttag ttgcagtagg gtattagttt atagaagaat ctaaaagtga
     2821 cagagacatt actatagcaa tttctccatc tcttttcgag aaaacgtgta tgttagctat
     2881 caaatatttt tgattgttca tcagattgag gattttcaaa ttgttcataa gaaaataata
     2941 aaatgttttc tccagtaact tgcggttttt cttgagtaat ttgtagtttt cctcgagtaa
     3001 cttgtgattt agagtggata gctgaaaaat gaaatatatg gtgagtattt tttaggcgta
     3061 ctcattaaat tcactcaaaa agtctttaga tctaaaataa agttatttgt tcccactttt
     3121 gaaatactaa attgcatgtt ttgaaatacg caattacatg ttttgaagga ttgcacttaa
     3181 agttaaaaag tttagctggt agtactgttt tgatgactcg ctccagaaat ccaaaaatat
     3241 aatgatacag taattgggat aatatgtaat caatttacaa atttaaaaat atctcaataa
     3301 caaaacgtga ccatcaaaat tacaataagc atacttgtat ataagaaaaa gaaaaattat
     3361 ttcaaagatt tattttattc caaaatcttt atttaactat aggtaaatgg gaaaaactta
     3421 aaagtttaaa cataaaagta gacggaagat gtagtatacg gaaacaatta atgtttcagg
     3481 aaatcttttt aagtcaaata ttgattatga aagtcatact gctaaagcta caaaaaaact
     3541 ttttaataat aatttttaaa atagacaagc tagacgtcta gacgcttgtc gcgcacaaca
     3601 acactgatat tgttgccacg gatgggtttg tttttttcag tttccttaaa aaaaaattat
     3661 attttcaaag ccattgtatg tgttcttcac atttaatgag gagaaataag ttttaataaa
     3721 aaatgttcct tcctgaaata actaattcaa ttacacttta ccggaaaaag aatatgtaat
     3781 aaattctata gtaaaagttt ttattaatca ttaatgatac gaatttaaat tctcttaaac
     3841 tcatgttaaa acaaccagaa acatctaatc ccggggaaaa aatgagtaaa ggagaagaac
     3901 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     3961 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     4021 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttctgttatg
     4081 gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg
     4141 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     4201 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     4261 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     4321 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     4381 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     4441 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     4501 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     4561 ctgggattac acatggcatg gatgaactat acaaatagca ttcgtagaat tccgataaga
     4621 caaatctgaa cttttattta aaatccgaag aaatacaaaa attgcatgta atatttttat
     4681 aaaattataa attaaaagat tgttattttg ttaggatttg ttaaaatttt ctttgacata
     4741 ttgagcaata ttgtctaaac aaaataaaca taaagtttat tgaaaatttt taattttaac
     4801 ttcatataat tgaaaattgc cttagatgca catagactca tatttcgtcg gtttttaaaa
     4861 tattcttttc gattttttat cgagttttta aaattgcact ttaatttaac ctctaatttc
     4921 acctaaattt ttgtaaaagt tgatttgagt aaaaaaaaaa aacttttttt ttttttttgg
     4981 gtcactccat tcggggccga aattgtgaat gtattaagct ttaaataaat taatgtatca
     5041 gatttattaa aataagtgta caggattttt agagaattat aaataaaaca ggttttttct
     5101 cattaaataa taattgcgta aatatgtaaa aggtatacac tttaaaacaa ccgccattaa
     5161 t
//
