LOCUS       62056_1276        5363 bp DNA     circular SYN 23-JAN-2019
DEFINITION  Expression vector Hym-176B_EGFP DNA, complete sequence.
ACCESSION   LC426373
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Noro Y, Yum S, Nishimiya-Fujisawa C, Busse C, Shimizu H, Mineta K, 
            Zhang X, Holstein TW, David CN, Gojobori T, Fujisawa T.
  TITLE     Regionalized nervous system in Hydra and the mechanism of its 
            development
  JOURNAL   Gene Expr. Patterns (2019) In press
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Noro Y, Fujisawa T, Shimizu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2018) Contact:Yukihiko Noro Waseda University, 
            Center for Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho, 
            Shinjuku-ku, Tokyo 162-8480, Japan
REFERENCE   3  (bases 1 to 5363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene Expr. 
            Patterns (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2018) Contact:Yukihiko Noro Waseda University, Center for 
            Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho, 
            Shinjuku-ku, Tokyo 162-8480, Japan"
FEATURES             Location/Qualifiers
     source          1..5363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        362..466
                     /label=AmpR promoter
     CDS             467..1324
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1498..2086
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2374..2395
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2410..2440
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2448..2464
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2472..2488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4086..4799
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
       61 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      121 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
      181 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
      241 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
      301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      361 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      421 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      481 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      541 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      601 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      661 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      721 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      781 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      841 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
      901 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      961 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1021 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1081 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1141 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1201 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1261 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1321 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1381 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1441 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1501 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1561 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1621 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1681 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1741 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1801 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1861 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     1921 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1981 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2041 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2101 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2161 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2221 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2281 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2341 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2401 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2461 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat gcctgcagct
     2521 tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt gctggaattc ggctttcgta
     2581 cgagaatcgc tgtcctctcc aacgagccaa gaagtaacaa ctaagaaaga aaatagctaa
     2641 tttaatattt gtaactcaat actgcccaat ttgttcagcc atttagagat tagaaccttt
     2701 taaatttgtt gatatattat acaatactat attgtaaaaa aagatatata ctttacgtat
     2761 atttcaaagg tacccaaagc gattaataat ttttttttgt agcttttata gcaaaaaaca
     2821 taattgaaac aaatcaaaaa taagaaagaa aatatttata taaaaacttt ttgataattt
     2881 tttttgttgg tttttttttc ttgcacatgc ggaaccctca gcaaatattt gcttttgcaa
     2941 ataattgctt acataaaata catacttaca taaagcaaat aattgtttac ataaaaatcc
     3001 ataaatacgt ttttcgacat ttttatgctt aaacgtttta aactgaaaaa cgcaattttt
     3061 cagtttaaaa cgtttaaaac gcatttttat gcttttcgtt taaaacgcaa attgtagata
     3121 acaaaatgtt tttttttttt ttttttcaga tttaaaaata tgcgcctaaa atctgcacct
     3181 atttcataaa aactagctgg gtatattaaa attaggattc tgggtaattt agtaactaaa
     3241 aattacccag aacccttatt ttttatgtaa ccaattgttc aaaacttatg aaaaattaac
     3301 cagaatcctt atctttaata taatcaaaag tttaaaactt ttgaattttt ttactgggtt
     3361 gttggaactc attaaaaata aaatcaaaag ttttaaacaa aaataaattg tttatttttt
     3421 ctttataacg agagggacag ttacattata ttactaatta gactttactt aaaaatgcag
     3481 acaaaatata aattaataaa aaaaaaaagt ttacaacacc aggatacgga gattaaccaa
     3541 tcataaaaat aatgtaaacc agattaatta tcaataatac tacgacacct gtgatcattt
     3601 tatactttta tattcattgt aaacgagttt attatataca atcacaaagt tgcaatgata
     3661 aaagaataat aacaacaaca acaacaaaac catactgtta aagttagtaa agagtttgca
     3721 attcattaaa ttgagtaacg agttaaatat ttaaacgagc aaggctgccc acgtgaatac
     3781 ataaatcaca tcactggcga gacttgtttt ttaattttaa aaaaaaatta gttttttatt
     3841 gtaagttttt ctgtgttgcc ttttgagcac cgattgaaaa ctgactatta cagagagcta
     3901 tacaatagaa tcttttgtaa aagttttaaa tgtatttttt attctttgtt taaaataata
     3961 ctttaaattc acttaaattc gaaattaaaa tcaaatcgaa aaactcaacg aagcagttaa
     4021 aaagtactca acataaaatc attttgaagc aaagaaaaag aaagaagtaa aacgaagggg
     4081 aaaaaatgag taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     4141 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
     4201 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
     4261 cacttgtcac tactttctgt tatggtgttc aatgcttttc aagataccca gatcatatga
     4321 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
     4381 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
     4441 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
     4501 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
     4561 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
     4621 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     4681 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
     4741 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
     4801 agcattcgta gaattcaacc tggagatcaa acacaaaaaa aatcataaat tttgtgtatg
     4861 tgatattgat gttaacataa attataaaaa tttgtgaaaa aatatcataa tacaatttac
     4921 ttaatcttga ctacctatgt cattttaatt ttttttattt tatttactta tcgtaatcgt
     4981 ttagttaaaa aatttttgtt ctttaaaaaa atcttgatgt aacctttcaa taaaaagtat
     5041 aaccagtgct tgtaaataaa aaattagtaa atccctccca gtaggataca gtcgtaaact
     5101 tatactgatg caaagatatt actgataatg atttatatgt tttatttcaa gtaggagaag
     5161 tgcagcaaca tctacagatc cagccgttca gccctgggtt caaaaaactt tagttgcggc
     5221 ggcgtagaag ttttctgcaa cccaaacgtt gttatcgtac attatttgaa ccacataaac
     5281 taaatgttat gctgtaatga tgtagtaata gcgcgttcct tttataagcg aaaaataata
     5341 agtttaatat ttaccgcatc tct
//
