LOCUS       62056_1271        4700 bp DNA     circular SYN 23-JAN-2019
DEFINITION  Expression vector Hym-176A_EGFP DNA, complete sequence.
ACCESSION   LC426372
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Noro Y, Yum S, Nishimiya-Fujisawa C, Busse C, Shimizu H, Mineta K, 
            Zhang X, Holstein TW, David CN, Gojobori T, Fujisawa T.
  TITLE     Regionalized nervous system in Hydra and the mechanism of its 
            development
  JOURNAL   Gene Expr. Patterns (2019) In press
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Noro Y, Fujisawa T, Shimizu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2018) Contact:Yukihiko Noro Waseda University, 
            Center for Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho, 
            Shinjuku-ku, Tokyo 162-8480, Japan
REFERENCE   3  (bases 1 to 4700)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene Expr. 
            Patterns (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2018) Contact:Yukihiko Noro Waseda University, Center for 
            Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho, 
            Shinjuku-ku, Tokyo 162-8480, Japan"
FEATURES             Location/Qualifiers
     source          1..4700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        362..466
                     /label=AmpR promoter
     CDS             467..1324
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1498..2086
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2374..2395
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2410..2440
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2448..2464
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2472..2488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3671..4384
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
       61 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      121 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
      181 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
      241 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
      301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      361 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      421 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      481 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      541 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      601 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      661 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      721 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      781 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      841 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
      901 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      961 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1021 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1081 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1141 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1201 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1261 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1321 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1381 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1441 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1501 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1561 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1621 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1681 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1741 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1801 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1861 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     1921 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1981 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2041 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2101 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2161 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2221 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2281 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2341 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2401 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2461 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat gcctgcagct
     2521 tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt gctggaattc ggcttatgac
     2581 atgattatag cttcttgtaa acttaatcac tatttaggtg tttaaactct attcattcca
     2641 tattaaaact cgtcatcaac ttagcatact tccgacaata attttatttg caatctttag
     2701 ttgattctaa tgagcaatat aaagaggtgg aataactaat ttttattttt accgctaatc
     2761 taaagttatt tatgtttaat aactcaatta tattttatta caaagtgagc gtctttgttt
     2821 gtcgctactt tgaattatca tttcgtattc tatttattct aatgaggaaa gccggtgaga
     2881 acatatatta ataatgaatt acattcagat aaataacatg tgggtttttg aaaaaaaaag
     2941 taaattctaa acaaaataca aaaatattta ttttttatta aagtaacata tttaaaacaa
     3001 gtttgtattt ttatgaaata tacgtagctt gaggttacgt tgactcattt tattttaaat
     3061 caaacatttc aaacaattac tcccaaagtt ataactaaat tgtttttctt ttaaacaatg
     3121 acattttaac cattattaac aaatataatt taaacaaaaa aagtagacgg aagataagca
     3181 aaaaacaaac aaactggaaa caggtaatga atccaagttt tttggcaaag attaaatatt
     3241 ttattaaaaa gtgcaaaaag ttataagtaa gctctcatat taaattagtt agacgtctag
     3301 atgcaaattg taatatttta taaaaacaaa ccttaatatc gtgttcagag gcgagactgt
     3361 ttttttaatt tcctgagaat cattttttca ttgtgcgtat ttttataggt tgccttatat
     3421 gcaataggag gaaatcaatt ttaacaataa atgttccttc ctgaaacaat gagttttatt
     3481 tcgctttcgg aggaaacaaa gttacacaat agaatctata gtaaaagttt tttattattt
     3541 attaaaaata tgactttaaa ttcccttaat cttacattga aacaatcaga aacagaaaat
     3601 cgatctagct agttgaaaaa ctctcgacac ataattatta caaagtaaag aacgaagtcc
     3661 cggggaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     3721 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     3781 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     3841 gccaacactt gtcactactt tctgttatgg tgttcaatgc ttttcaagat acccagatca
     3901 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     3961 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     4021 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     4081 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     4141 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     4201 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     4261 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     4321 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     4381 caaatagcat tcgtagaatt caacctggag atcaaacaca aaagaatttg taacttcatt
     4441 tagtttttca attaccttta tgctatttat ttaatgtaat catgtgatat ttgtataatt
     4501 ttaaagattt attaagataa gattagttct aactacactg ttgttgtttt ttttattaaa
     4561 aataaaatcg aattaaaaat aacaactgca ttttaataca atgccatttt ttattaggaa
     4621 aaagccactt ttaaatataa tccctagagt ttagtgccat cagttggtgt cttaattacc
     4681 aaagtaatac agtttaatgt
//
