LOCUS       40924_1304        9524 bp DNA     circular SYN 15-JUN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   xsan
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9524)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label=AmpR promoter
     CDS             209..1066
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1240..1828
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2014..2156)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        4010..4028
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4117..4139
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4159..4185
                     /codon_start=1
                     /label=9xHis
                     /note="9xHis affinity tag"
                     /translation="HHHHHHHHH"
     CDS             4198..5298
                     /codon_start=1
                     /label=MBP
                     /note="maltose binding protein from E. coli"
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     CDS             5353..5373
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQS"
     CDS             5380..7302
                     /codon_start=1
                     /label=Cas8
                     /note="accession number:  WP_001194012.1"
                     /translation="MQTLKELIASNPDDLTTELKRAFRPLTPHIAIDGNELDALTILVN
                     LTDKTDDQKDLLDRAKCKQKLRDEKWWASCINCVNYRQSHNPKFPDIRSEGVIRTQALG
                     ELPSFLLSSSKIPPYHWSYSHDSKYVNKSAFLTNEFCWDGEISCLGELLKDADHPLWNT
                     LKKLGCSQKTCKAMAKQLADITLTTINVTLAPNYLTQISLPDSDTSYISLSPVASLSMQ
                     SHFHQRLQDENRHSAITRFSRTTNMGVTAMTCGGAFRMLKSGAKFSSPPHHRLNSKRSW
                     LTSEHVQSLKQYQRLNKSLIPENSRIALRRKYKIELQNMVRSWFAMQDHTLDSNILIQH
                     LNHDLSYLGATKRFAYDPAMTKLFTELLKRELSNSINNGEQHTNGSFLVLPNIRVCGAT
                     ALSSPVTVGIPSLTAFFGFVHAFERNINRTTSSFRVESFAICVHQLHVEKRGLTAEFVE
                     KGDGTISAPATRDDWQCDVVFSLILNTNFAQHIDQDTLVTSLPKRLARGSAKIAIDDFK
                     HINSFSTLETAIESLPIEAGRWLSLYAQSNNNLSDLLAAMTEDHQLMASCVGYHLLEEP
                     KDKPNSLRGYKHAIAECIIGLINSITFSSETDPNTIFWSLKNYQNYLVVQPRSINDETT
                     DKSSL"
     CDS             7274..8332
                     /codon_start=1
                     /label=Cas7
                     /note="accession number: WP_000775488.1"
                     /translation="MKLPTNLAYERSIDPSDVCFFVVWPDDRKTPLTYNSRTLLGQMEA
                     ASLAYDVSGQPIKSATAEALAQGNPHQVDFCHVPYGASHIECSFSVSFSSELRQPYKCN
                     SSKVKQTLVQLVELYETKIGWTELATRYLMNICNGKWLWKNTRKAYCWNIVLTPWPWNG
                     EKVGFEDIRTNYTSRQDFKNNKNWSAIVEMIKTAFSSTDGLAIFEVRATLHLPTNAMVR
                     PSQVFTEKESGSKSKSKTQNSRVFQSTTIDGERSPILGAFKTGAAIATIDDWYPEATEP
                     LRVGRFGVHREDVTCYRHPSTGKDFFSILQQAEHYIEVLSANKTPAQETINDMHFLMAN
                     LIKGGMFQHKGD"
     CDS             8566..8934
                     /codon_start=1
                     /product="type I-F CRISPR-associated endoribonuclease
                     Cas6/Csy4 [Vibrio cholerae]"
                     /label=Cas6
                     /note="accession number:  WP_000872476.1"
                     /translation="MEQLQYFHISNTVLVPEDCTYVSFRRCQSIDKLTAAGLARKIRRL
                     EKRALSRGEQFDPSSFAQKEHTAIAHYHSLGESSKQTNRNFRLNIRMLSEQPREGNSIF
                     SSYGLSNSENSFQPVPLI"
     terminator      9074..9121
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(9487..9515)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa
     4081 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
     4141 atacatatga aatcttctca ccatcaccat caccatcacc atcaccatgg ttcttctatg
     4201 aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc
     4261 gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg
     4321 gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc
     4381 ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc
     4441 ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac
     4501 ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat
     4561 ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa
     4621 gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg
     4681 attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac
     4741 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa
     4801 aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc
     4861 gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg
     4921 aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc
     4981 gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc
     5041 gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt
     5101 gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccact
     5161 atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg
     5221 tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc
     5281 ctgaaagacg cgcagactaa ttcgagctcg aacaacaaca acaataacaa taacaacaac
     5341 ctcgggatcg aggaaaacct gtacttccaa tccaatgcaa tgcaaactct gaaagaacta
     5401 atcgcatcca atcctgacga cttaacaact gaacttaaga gagcatttcg tccactcaca
     5461 ccgcatattg caattgatgg taatgaactt gacgcactga cgatattagt caatttaacc
     5521 gataagactg atgatcagaa agacctgctc gatcgagcca aatgcaagca aaaacttcga
     5581 gatgaaaaat ggtgggctag ctgcataaat tgcgttaatt acagacaaag ccataaccca
     5641 aaattcccgg atatacgttc tgaaggcgtg attcgaaccc aagccctggg tgaattaccg
     5701 agtttcctac tctcgtcctc aaaaatccca ccataccatt ggtcatatag ccatgattca
     5761 aaatacgtca acaaaagcgc attcctcacc aatgagtttt gttgggatgg tgagatctca
     5821 tgtttgggtg agcttcttaa agatgcagat cacccacttt ggaatacttt gaaaaagtta
     5881 ggttgttctc aaaaaacttg caaagcaatg gcaaaacaac tagctgatat tactctcacg
     5941 actatcaatg tcacactcgc accaaattac ctgactcaaa tatctctccc cgatagtgat
     6001 acatcctaca tttcactctc tcctgttgca tcgctatcga tgcaaagcca ctttcatcag
     6061 aggctccaag atgaaaatcg gcatagcgcg ataacgcggt ttagccgaac taccaacatg
     6121 ggggtcactg cgatgacatg tggcggcgca tttagaatgt taaagtctgg cgctaagttt
     6181 tccagccccc ctcatcaccg attaaacagt aaacgaagtt ggttgacgtc agagcatgtt
     6241 cagtcattaa aacagtacca gcgcctcaac aaaagcctca tacctgaaaa ctctcggatt
     6301 gcactccgta gaaaatacaa aatcgagctt caaaatatgg tcagatcttg gtttgcaatg
     6361 caagaccata cccttgattc gaacatactt atccaacatt tgaatcatga cctatcttac
     6421 ttaggagcca caaaacgttt tgcatacgat ccagcgatga ccaagctctt tactgagctt
     6481 ttgaaacgag agttatcaaa ttcaatcaat aatggtgagc aacacactaa tggatcgttt
     6541 ttagtcctac cgaatatcag ggtttgtggc gcaacagctt taagctcccc ggtaacggtg
     6601 gggattccat cacttacagc tttctttggc ttcgttcacg catttgaacg gaatataaat
     6661 cgcaccacct catcgtttcg tgttgaatcc tttgcgatat gcgtccatca actacatgtc
     6721 gagaagcgag gtttgacagc agagtttgtg gaaaaaggcg acgggactat atccgctccc
     6781 gcgacccggg atgactggca gtgtgatgtc gtatttagcc ttattttgaa caccaacttt
     6841 gctcaacata ttgaccaaga tacgttagtt acatcactac caaagcgatt ggctcggggt
     6901 tcagcaaaaa ttgcgattga tgactttaaa catatcaact cattctcgac attagaaaca
     6961 gcgatcgaat ctctgccaat agaagctggt aggtggttat cactttacgc acagtcaaac
     7021 aataatctaa gtgatctatt agcagccatg acagaggacc atcagctcat ggcaagctgc
     7081 gtcggttacc acttgttaga agagcccaaa gataaaccaa actccctcag aggttacaaa
     7141 cacgctatcg ccgagtgcat cattggactc attaactcaa tcacctttag ctcagagact
     7201 gatcccaaca caatcttttg gtcgctaaag aactatcaaa actacctagt ggtacagcca
     7261 aggagtatca acgatgaaac taccgacaaa tctagcctat gagcgctcta tcgacccatc
     7321 agatgtctgt ttttttgtcg tctggcccga tgatagaaaa acacctttaa cctacaattc
     7381 tcgtactctg ctcgggcaaa tggaagcggc atcattagcc tatgatgtct caggtcaacc
     7441 aataaaaagt gccaccgctg aggcgttagc tcaagggaac cctcatcaag ttgatttctg
     7501 ccacgttcca tacggtgcga gtcatattga atgcagtttc tccgtctcgt tttcttctga
     7561 actacgtcaa ccatataagt gtaactcaag caaagttaaa caaacgctag tgcaattagt
     7621 cgagctctac gaaacgaaaa tcggctggac tgagctagca acccgatatt tgatgaacat
     7681 ttgcaacggt aaatggctgt ggaaaaatac ccgtaaagcc tattgctgga acattgtact
     7741 tacaccttgg ccttggaacg gggaaaaggt tggatttgaa gatattcgta ctaactacac
     7801 ctcacggcaa gactttaaaa ataataaaaa ttggtctgct atagttgaaa tgatcaaaac
     7861 cgcattttct agtactgatg ggctggcgat atttgaagtc agggccacct tgcacttgcc
     7921 aacgaatgct atggtgcggc caagccaagt tttcacagaa aaagaaagtg gcagtaaaag
     7981 taaatctaaa actcaaaaca gtcgagtttt tcagagtaca actattgatg gtgaacgatc
     8041 gccaatacta ggggccttta aaacgggagc agctattgca accattgacg actggtatcc
     8101 tgaagccact gagccactaa gggtcggacg gtttggggtt catcgcgaag atgtcacttg
     8161 ctaccgtcat ccgtctaccg gaaaagattt tttctcgata ttacaacaag cagagcacta
     8221 tattgaagtg ttgagcgcca acaaaactcc cgctcaagaa actatcaacg acatgcactt
     8281 tttaatggct aacctgatta agggtgggat gttccagcat aaaggagact gactgtgaaa
     8341 tggtattata agacaatcac ctttctgcca gagttgtgca acaacgagtc actggctgca
     8401 aagtgtctcc gcgttctgca tggatttaac tatcagtatg agacacgaaa tatcggcgtt
     8461 tcatttccgc tttggtgtga tgcaacggtt ggaaaaaaga tttcatttgt cagcaagaac
     8521 aagatagaac tcgacttact acttaaacaa cactatttcg tccaaatgga acaacttcaa
     8581 tactttcata tatccaacac tgttctcgtc ccagaagatt gtacatacgt ttcctttaga
     8641 cgctgtcaat ctatagataa gctcacagca gcagggctgg caaggaaaat cagacgcctg
     8701 gagaaacgtg ctctatctag aggcgagcaa tttgacccat catcttttgc tcaaaaagag
     8761 catactgcaa tagcgcacta ccactcactt ggggagtcca gcaaacagac gaaccgcaac
     8821 tttcgactca atatcaggat gctctcggag caaccccgtg aggggaactc gatttttagt
     8881 agctatggct tatcaaattc agaaaactcg tttcagcctg tacccttaat ctaatagcat
     8941 tggaagtgga taacggatcc gcgatcgcgg cgcgccacct ggtggccggc cggtaccacg
     9001 cgtgcgcgct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     9061 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     9121 gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt
     9181 agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg
     9241 acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca
     9301 cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta
     9361 ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga
     9421 gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac
     9481 taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
//
