LOCUS       62056_1176        6852 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2581..2667
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2759..2786
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2805..2896
                     /label=AmpR promoter
     CDS             2897..3754
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3928..4516
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4702..4842)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(5109..5984)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHFFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             6080..6097
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             6101..6133
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     CDS             6137..6160
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     CDS             join(6179..6852,1..2371)
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKNQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
ORIGIN
        1 tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta
       61 cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc
      121 tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct
      181 gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt
      241 tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg
      301 cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg
      361 cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt
      421 gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc
      481 gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc
      541 caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct
      601 accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt
      661 gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg
      721 ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac
      781 cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc
      841 ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct
      901 gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg
      961 gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca
     1021 gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg
     1081 cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc
     1141 gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg
     1201 gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca
     1261 ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc
     1321 tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca
     1381 actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca
     1441 catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc
     1501 gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa
     1561 taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa
     1621 aaaccaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat
     1681 tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc
     1741 gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc
     1801 ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg
     1861 gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc
     1921 gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga
     1981 gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc
     2041 ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag
     2101 cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg
     2161 cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg
     2221 ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat
     2281 tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccagctga gcgccggtcg
     2341 ctaccattac cagttggtct ggtgtcaaaa ataagcttgg ctgttttggc ggatgagaga
     2401 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
     2461 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
     2521 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
     2581 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2641 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
     2701 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
     2761 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta
     2821 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2881 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2941 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3001 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3061 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3121 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3421 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     3721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     3781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     4081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4441 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4561 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4621 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4681 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4741 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4801 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     4861 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4921 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     4981 cagcagatca attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac
     5041 gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt
     5101 accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
     5161 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
     5221 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
     5281 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
     5341 aaaaaatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
     5401 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
     5461 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
     5521 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
     5581 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
     5641 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
     5701 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
     5761 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
     5821 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     5881 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
     5941 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
     6001 ccattcagag accggtcgtt ttgcggccgc tttgggctcg agctaacacc cgggggagga
     6061 attaaccatg gggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg
     6121 acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatccaatga tagatcccgt
     6181 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6241 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6301 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
     6361 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa
     6421 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt
     6481 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt
     6541 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc
     6601 gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc
     6661 tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct
     6721 gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt
     6781 ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac
     6841 tcgctttaat ga
//
