LOCUS       40924_1309       12538 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector HKBS1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12538)
  AUTHORS   Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
  TITLE     Integration-proficient plasmids for Pseudomonas aeruginosa: 
            site-specific integration and use for engineering of reporter and 
            expression strains
  JOURNAL   Plasmid 43 (1), 59-72 (2000)
  PUBMED    10610820
REFERENCE   2  (bases 1 to 12538)
  AUTHORS   Becher A, Schweizer HP.
  TITLE     Integration-proficient Pseudomonas aeruginosa vectors for isolation 
            of single copy chromosomal lacZ and lux gene fusions
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 12538)
  AUTHORS   Becher A, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2000) Microbiology, Colorado State University, 
            Fort Collins, CO 80523, USA
REFERENCE   4  (bases 1 to 12538)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 12538)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2000"; volume: "43"; issue: "1"; pages: "59-72"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (03-APR-2000) Microbiology, Colorado State University, Fort Collins,
            CO 80523, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(238..285)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    495..521
                     /note="phage CTX attachment site; core sequence"
     promoter        827..845
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(1568..2677)
                     /label=LuxE
                     /note="LuxE"
     CDS             complement(2859..3839)
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             complement(3857..4936)
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             complement(4988..5908)
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             complement(5923..7362)
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     misc_feature    7822..7893
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(7860..7876)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7902..7920)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(8094..8141)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     oriT            complement(8348..8456)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(8615..9784)
                     /codon_start=1
                     /gene="int"
                     /product="integrase"
                     /label=int
                     /note="modified integrase"
                     /protein_id="AAG09988.1"
                     /translation="MADGVEVRGKRIRIYFRYQGELCRESIPGDATPENIANAERLAGI
                     INYEIKQGVFSYSRHFPDSPRVKSNTLGHYIDLWLDIKRNQIAASGFRGYTSRVETHIR
                     PRWGDSQADSIDHLDIQDWVQNTLMPKLHNKTVREIVSNLRQIFRLYRTRNRSAHDPTD
                     GIVITLPDADDPDPFTREEIDLILGTETARIGELNLAEFMIWSGPRVSEAIALAWEDVD
                     LDTGTVVFRRARVRSQYKVTKTRRSTRKVQLLAPALRALQQQAKLTRRLPPVQIEVIDR
                     DNRTRKPQRVRFVFHNSASGAAYSTSDTLRNGWWHGHLRNAGVRSRGPNQCRHTFASQM
                     LSSGIATPEWIADQMGHTSTAMIFKHYAKWISKDGPDIVGLLNQALKLS"
     gene            complement(8615..9784)
                     /gene="int"
                     /label=int
     rep_origin      complement(9881..10469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11294..12481)
                     /label=TcR
                     /note="tetracycline efflux protein"
ORIGIN
        1 atcgatgata agctgtcaaa catgagaatt aattcatgtt ctttcctgcg ttatcccctg
       61 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      181 ctctccccgc gcgttggccg attcattaat gcagcgaatt agcttcaaaa gcgctctgaa
      241 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat cccctgattc
      301 cctttgtcaa cagcaatgga ttttcaatgg gtcaacatga aatccggctg ttgccctggg
      361 acggcaggtt tgacggcagg cttccaggct ctctcgcaca cgctgggaac actctgggaa
      421 caaacgcgag tgaaaatgga cagtagggcc caggaataat gcggcctcca gcgaggttgg
      481 ggcgtttcat atgagttcga atctcaccgc ctccgccata taacctgctg ttttcattga
      541 tgtttccatt cgacccactc tcaggagtga acgatttggg aacaaactgg gaacgaggcg
      601 cagaaaaaag gggccccgca gggccccttt ttcgttgctt caggaaagtt tcagcgcctg
      661 attcagcaga cctacgatgt cggggccgtc cttgctgaat tagctttatg cttgtaaacc
      721 gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca attaattagt
      781 aatataatct attaaaggtc attcaaaagg tcatccaccg gcgcgcaatt aaccctcact
      841 aaagggaaca aaagctggcc caattccttt aatcccttta attcctggat tttttctccg
      901 aaatctggga gcgtataaac atctggactc gtataaatca gttgactggt ttctatgcct
      961 tctttgccac atattatatg tatatcaact aataccgtat tattatcata acctatagac
     1021 atagttccat tgtattttag cgcgttctca aattcttctt ttgatggata cgatgatcca
     1081 tctccgtact ttttcgttaa cgatttgctt actggcttca caagtctgcc aatattaatc
     1141 tctaatcttt taaatttccc atttaaaaaa aatgccattg cttcacgctt tttgttagca
     1201 aacgaaaagt cagaacatga ataaagtgat acttcttcta ttcgagaatc tccctcaaat
     1261 ttttcaaaat cacataacct cttcgaaaga aactcttctt ctgtcatgcc aaactttgca
     1321 tctttatacc catcaactgc cattgcacca aagctaatca ttcccaatgc aagcataact
     1381 gccaattttt tcatgttatc tccttttgct ttgagccaat aataacaatg agaacaatgt
     1441 taagccaacc ccataaacct ctatcaagta cccaaatgtg gatgttttgg agaattttgt
     1501 aaagtgtaga tgtcattcag atttatataa cgcaagcatt ccacttacaa ttaggcaaag
     1561 gatatcaact atcaaacgct tcggttaagc tcaaagcaca ccctttctgc gtcctcgtat
     1621 tgacgcgacg taaaatttca acgagcacgc cgggatactt accatattct ctgctaatta
     1681 tcccgacatc atcggtaaca ataaatgctg gataactggt tgctgacgca tccatataac
     1741 tcatcaaccc cggcgttcca tcaggtacag gtttcaacgt ttcaggatca agcgctcgcg
     1801 catataccca cggcggaaca tgtttacgct gcatttcatc ctcaaagaaa caagtgttga
     1861 gttcaacttg attaaatata tctcggatct gactaatatc actgagattg aaagtatcaa
     1921 ataaaagatg attgaaatca tcacgtttca gagattcttt ttcgtaactt ttccagccgc
     1981 ctccggttat gatataaagg cttttatctc cagaaaatga gattttttta tctttcatat
     2041 aatggcagag taaataaata aagtatggcg aaccaataag acaaagatct ttcccttgat
     2101 tttttattcg ttcaagacta ttcaatgttt taacaaaatc tattcgttct tctgttacgg
     2161 taaatgtcgt aggatataac aattccacca aactcataac atatttaaac caaatattat
     2221 gagcattaaa tctatctggt cccaaattga ctaattctat ttgatgatca aaccaactac
     2281 caacatattt catgccataa ctcacagagc ctaagagtct ctcaatactt aatctgtcac
     2341 gcgccacctg actttttaaa ccattcgtgc cgctactggt aaaccaactt tcaatctcgt
     2401 tttcctgaga agttaataag cgagtaaact taaaaaccga tgttgggaat acaggtatgt
     2461 catcaatttc cgtaatattg tcatctactt tgtgtgcctg acagtagtga cgatattctc
     2521 gacaatgttt ataatgatta cgaaatgcat caagcacaag tttctttctg attttttcct
     2581 gctcgtcgta agaccacact aatggatcgc tcgaaaaaat caaatcatca atttctgagc
     2641 ttgctgtaat ttcttgttta tcaacatatg aagtcatacc tgttttactc ctcaagataa
     2701 tattagaaag tatggcagca ctgctgtcat actcttttat acccttcatc tttcaagctg
     2761 ctgctttgtt ggctgctttc actcacccca gtcacatagt tatctatgct cctggggatt
     2821 cgttcacttg ccgcctcgct gcaactcgaa atctattagg tatattccat gtggtacttc
     2881 ttaatattat catcaacaat attgattaca tttttttggc tcatcaaatc attcattggt
     2941 tcaaaggaca gcaatacact tttcgcacca cacttttcaa ttgccaactt agccgcagtt
     3001 atacactccg tataatttcc gacagcgttt tctgcaatta tttcttcaag tttattttcg
     3061 aaattttcat tagggtgcat ttcaagaaca taatcactaa taaatgcacg cgtctcttgt
     3121 ttagctttat tactatcttc gttatagtta actaatatca ttaactgatg gtctatctct
     3181 gataggtcaa cgtcatattt atccgcaacg gctttatatc tttcagcata ttcatatcta
     3241 acatcattag aatcatccca cttaaagatg agaggaatac cttttttggc cgcccactca
     3301 acaatatgat gactggttgc tgttacatat ttccgaggtc cgcctggcgt ataagcatgg
     3361 ggatttacag atattttagg gaagctataa aaatcgttat ctggattaca atagcctgtt
     3421 gttaaagcat cgttaatgat ttcataacac tcttcaaata gttgctgttg atattcaacc
     3481 gggcgattaa aaaaatgcat ttcatctttt ttttcgcaat cactaaaccc taaaataaat
     3541 ctcccttcac ttaactgatc caataagcac gcttcctccg ctatggcgac aggatgatga
     3601 gttgtaatga tgtgatttaa tgaaccaatt ttaattttct ctgttaaacc gagcagaaaa
     3661 ccagaaacag tcagaggagc gccgacaaca ccattatctg aaaaatgatt ttcatacact
     3721 aaaatctgtt caaaattcaa cttatcaaca tactccgtta tttcctgcat acgaactata
     3781 ctttgttctt gaacagttgt tgaattgatg aagttaagga agaacaatcc aaatttcatt
     3841 tctttctcct tagctaatat aatagcgaac gttgtttttc tttaagaaat ggcatgacat
     3901 cagactggaa gagcttcatg gaagcaataa tttcgtctac tgttccatta gcttcaaatc
     3961 cacaacaaat atttgatatt cctgtagcat caatgtcttt ttgaattatg tcaatacatt
     4021 cctgcggcgt tcccacggga ttgatttcgt aactgtaatc aatacggcga ttagtatctt
     4081 tatgtccttt taatacaaag tcacgccact gccctttatt gaaatcataa cctcttgttt
     4141 ggtctgaatc atcaaaaata gtcgtagcat tcacataaga atcataccaa tgccccagaa
     4201 atttccggca aatctctttc gctttaattg agtcatgatc tacagatgtt atatatgata
     4261 agcaatggtc gatattatga atatcgtgcc catattcttg agccacttca ttataaagct
     4321 caagttgtgc tttcttttcg ttagtattta taatccaact taatatcatc ggtaggccaa
     4381 attgagcagc ccactcagtc gtcgaagctg attcagccac cacataaacc ggtgcgccac
     4441 ctctgctata cgccgcgggg tttactttta ccttatggaa cttgatatgt tcattatcag
     4501 cttccatata tccctctgtc atgccattct ttatcagccc gtaccagcat tccgctaagg
     4561 cgcgactgtt attcatatct gtgccgaata cgcgaaagtc cttgttgtaa agccctcggc
     4621 aaataccaaa ccgaaatcgt ccttttgaca tttgatccaa taaattcaca tcttcaagtt
     4681 ggcgtactgg atgggctgtg ggaagaacaa tagcggcagt tcctacattc aattttttag
     4741 tcgcgccaag taaatatgca gcagcgacat aagggttacc aagcaaacca aactccgtga
     4801 aatgatgctc cagtaaccat acggtatcaa aaccacactc ctcagagatg cgacctaatt
     4861 taaccaaacg tttcattacc tctgtttgag aaaattgggg aggttggtat gtaagcaaaa
     4921 agtttccaaa tttcatagag agtccttata ttgctatttg agtgatagaa tatctcaata
     4981 gattttaaga cagagaaatt gcttgatttt caatctcaat tctcattcgg cgttcattga
     5041 ctgtcgcaat agttaaatgt tcaaatgacg gttcagtaat atcaacatca atatccagat
     5101 gatcattatc catcgcgata gcggctttcg taaccgattg ataaaaattg cgcaggacca
     5161 ctaaattttc actcaagtca tgcgaacttc ctaacaaaga atatatcttg catcgattac
     5221 tacgaatatt tgataacaat gtgataactt catcttgctt gacccaatta tcgttatttg
     5281 cagtaaaagc aataaacggt atatcaagat acatcatgtt attaattgta gaagctaaat
     5341 cttcccaacc aaaatcaaga caatctctcg caaagacttc agcacccaat ttatggcctt
     5401 caaaatccag attatccggc aattcattaa tgggtagact gagataatca aaccctaaag
     5461 ctctttcaag agaatatctt aagttaacaa caccgactgc ggtgattaaa aacgaagcat
     5521 tgatttcaga taggcttgca taagctatcc gcgcagataa gctggaagcc aacataccga
     5581 agttatttat ttttcgtgta gttaaccaat caaccactgc taacaagctc tgctttccta
     5641 tagacattgt aaattcatca attgtccctg aactcaatcc aacgtggtga agcgaatcat
     5701 agcggatcac atgaaatcca ttccgcgata aatattccgc cagaccagca aaatgatcca
     5761 tcctgcgggc aaaaccagac gcaataataa tggcattctt tctctttggg ctgttttctt
     5821 ctggcagcgt ttcccaaaca tgaatttttt tatttccttc aacacaaata acgtggtcga
     5881 tggttttata ttttgattca ttttccatac ttttacctat tatgggacaa atacaaggaa
     5941 cttatcttct tccaggaatc gagtctgttc tatttcaacc gcaacatcct tagccgtata
     6001 gttagatggc ctttcatgag aaatatatgt cactaatcgt tgcaacggtc tcattccgtc
     6061 atgagatcca ccaactcgaa atatgttatt cattcctgct tctacaatcc tttccgcacc
     6121 ttttaatgct aacgcatctc gatatttaaa tgatgactcc caaggaaaaa tagatatggt
     6181 ttgcgtctta tttttttgaa cataaggcaa tatttgctca atattatcga cgtgatgaag
     6241 gtacacacat ctgccaagtg gttgattaaa ttccacacct gcatttgact caataatcat
     6301 ccaacgttga tgaatatcca cctctacttt taatccagca aacaggcttt ctttttgaac
     6361 taaagaatag gccgcctttt catcaaaatc ttttttggca ttcggtaata tatgcgcata
     6421 tagattaagt ttttctatca acgctaactt aaattcctca taatgatttc ccatgtaata
     6481 tatgttttgg gcagaaaaac aagctcgctg atcgtaaaaa caaacatcat gagccgcacc
     6541 tgtcgctgcg gacgtcaaat caacaggatt atcgataatg caaagactct ttttagaacc
     6601 aaatttaatc acatcagcat aagatggcgc gtgctctacc gcccaattaa tcgcatctgg
     6661 ccctccccaa gcgacaataa catccgcatg tcgcataatt tcttttgcga gtgatgtatc
     6721 accttggtgg ggccaatata taacagataa agagcgcgtt atcggatgat tagggtctac
     6781 atcaataaaa cttaacgcta atgcattagc ggtaaaagga tcggttgacg atgtttttat
     6841 aatacactga ttcttagtta aaattgcgcg taatatagac atgatcccag ataatggaac
     6901 attacctgcc aacagatgta cagatttacc tttcggaaaa gcccgaacat aactttcatc
     6961 ctgaggtagc cattcatcca tgatatggcg agaaccaagt tcattttcta caacatcata
     7021 aaggccgcct ttagaacata aaatcataga tatccaattg gcctctagct tagccatttc
     7081 ttctgaatat cccatatatt tttttaagtc acgaatgtat gtcctgcgtc ttgagtattc
     7141 ttcatttttc catctttgcc ctaccgtata gagaaaattg acaatgttat gcaaccgtaa
     7201 ttcgttattt ccattacaat caataatgtt ttttacatga gagtcattca atattggcag
     7261 gtaaacacta ttatcaccaa aattaatgga ttgcactaaa tcatcacttt cgggaaagat
     7321 ttcaacctgg ccgttaataa tgaatgaaat ttttttagtc atatttgcca tccatttaat
     7381 gggggtaatt tcaacttaag ttgctgttta actatcttgc gaactggtcg taccaacact
     7441 tgcttcttat atttttgtgt tatgacgatg taaataacaa atatatagaa aatcagattc
     7501 tgactacgtg gttcctggcc tgtaactttc gtcataatta tgataataat ataaattttc
     7561 tgctcaaggt ttttaatcta actgaatgga tcgtcaatat aacgcctatt cccgttttaa
     7621 tatctgttaa aatagcaaag gaaaattttc atatcaacaa gatttgttaa tgaaaagttt
     7681 aaattaaaaa agtcatgctc ttctctaatg cgtgataata tttatgtaaa ttattatcct
     7741 gagtattatt tcacaaatac cgatagaaat agattgaata gcaatctaat ttttaccggc
     7801 agatttctaa agaagaattg gggatccccc gggctgcagg aattcgatat caagcttatc
     7861 gataccgtcg acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta
     7921 cgcgcgccgg tggatgacct tttgaatgac ctttaataga ttatattact aattaattgg
     7981 ggaccctaga ggtccccttt tttattttaa aaattttttc acaaaacggt ttacaagcat
     8041 aaagctaatt ccattgagta agtttttaag cacatcagct tcaaaagcgc tctgaagttc
     8101 ctatactttc tagagaatag gaacttcgga ataggaactt caagatcccc aattcgctgc
     8161 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8221 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8281 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgctag
     8341 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
     8401 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
     8461 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
     8521 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
     8581 cagcggaaag tataccttaa cctttttcgt tgcttcagga aagtttcagc gcctgattca
     8641 gcagacctac gatgtcgggg ccgtccttgc tgatccactt ggcgtaatgc ttgaagatca
     8701 tcgccgtcga ggtgtgcccc atctggtccg cgatccattc gggagtggcg atgccgctgc
     8761 tgagcatctg gctggcgaag gtgtggcggc actgattggg accacgcgag cggacgcccg
     8821 cattcctgag gtggccatgc caccagccgt tgcgcagcgt atccgacgtg gagtacgccg
     8881 ccccgctagc actgttgtgg aacacgaagc gtacccgttg aggctttctg gtccggttgt
     8941 cgcggtctat cacctcgatc tgtacgggcg gaaggcgtct ggttagcttg gcctgctgct
     9001 ggagtgcgcg cagcgccggg gccagtagct ggaccttcct cgtcgatcgg cgagtcttgg
     9061 tgaccttgta ctggctgcgt acacgagcgc ggcggaaaac cacggtcccc gtgtccaaat
     9121 cgacatcttc ccaggccaga gctatggcct cgctgacgcg ggggccgctc cagatcatga
     9181 actccgccag gttcagctca ccgatgcgcg cggtttcagt accgaggatg aggtcgattt
     9241 cctcacgagt gaatgggtct gggtcatccg catccggaag agtgatcacg atgccgtctg
     9301 ttgggtcgtg cgcgctccgg ttccgagttc gatacagccg gaagatctgc cgcaggttgc
     9361 tgacgatctc tcgcaccgtc ttgttgtgca gcttgggcat gagcgtgttc tgcacccagt
     9421 cttgaatatc caggtggtcg atgctgtcgg cctggctgtc accccagcga ggtcggatgt
     9481 gggtttccac gcggctggtg tagccgcgga agccgctggc ggcaatctgg ttgcgtttga
     9541 tgtcgagcca gaggtcgatg taatggccca aggtgttgct tttcacccgc ggtgagtccg
     9601 ggaagtgtcg gctgtagctg aagactcctt gcttgatctc gtagttgatg atgccggcca
     9661 gccgttcggc attggctata ttctcgggcg ttgcgtcccc tggtatggac tccctacaca
     9721 gctcgccctg gtagcggaag tagatcctga tccgttttcc gcgtacctcg actccgtctg
     9781 ccatctggtt cccctgatcc acaccaagcg ttcaagctgg catgtgagca aaaggccagc
     9841 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9901 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9961 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10021 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10081 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10141 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10201 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10261 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    10321 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    10381 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10441 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10501 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10561 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10621 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10681 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10741 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10801 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10861 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10921 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10981 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    11041 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    11101 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    11161 catccgtaag atgcttttct gtgactggtg agtcaagggt tggtttgcgc attcacagtt
    11221 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
    11281 cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc
    11341 agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg
    11401 cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg
    11461 cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
    11521 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
    11581 agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga
    11641 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
    11701 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
    11761 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
    11821 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
    11881 tgactgggtt gaaggctctc aagggcatcg gtcggcgctc tcccttatgc gactcctgca
    11941 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
    12001 catgtaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
    12061 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
    12121 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
    12181 cgtagagaat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa
    12241 gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg
    12301 gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga
    12361 tgctgtcgga atggacaata tcccgcaaga ggcccggcag taccggcata accaagccta
    12421 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
    12481 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcta
//
