LOCUS       V007450                 6414 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V007450
VERSION     V007450
KEYWORDS    HA-p62.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Fan W, Tang Z, Chen D, Moughon D, Ding X, Chen S, Zhu M, Zhong Q
  TITLE     Keap1 facilitates p62-mediated ubiquitin aggregate clearance via
            autophagy.
  JOURNAL   Autophagy. 2010 Jul 22. 6(5):.
   PUBMED   20495340
REFERENCE   2  (bases 1 to 6414)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6414)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy.
            2010 Jul 22. 6(5):."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     polyA_signal    complement(258..391)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(524..895)
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     promoter        complement(914..961)
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     promoter        complement(1009..1338)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(1352..1780)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(1826..2050)
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2097..2123)
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(2133..3452)
                     /gene="SQSTM1"
                     /label="Sequestosome-1"
                     /note="Sequestosome-1 from Homo sapiens. Accession#:
                     Q13501"
     primer_bind     complement(3585..3609)
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
     protein_bind    3619..3637
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(3640..3658)
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(3660..3863)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(3864..4243)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4415..4434
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     promoter        4509..4613
                     /label="AmpR promoter"
     CDS             4614..5471
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5645..6233
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     6387..6404
                     /label="L4440"
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
      241 agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac
      301 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      361 tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat
      421 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
      481 catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca
      541 cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct
      601 cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg
      661 accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg
      721 gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg
      781 cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg
      841 ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt
      901 agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa
      961 cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc
     1021 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc
     1081 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     1141 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     1201 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     1261 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     1321 acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc
     1381 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     1441 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     1501 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     1561 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     1621 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     1681 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     1741 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc
     1801 cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta
     1861 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
     1921 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
     1981 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
     2041 gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctctagactc gagtcaagcg
     2101 taatctggaa catcgtatgg gtagcggccg cgcaacggcg ggggatgctt tgaatactgg
     2161 atggtgtcca gagccgctcc gatgtcatag ttcttggtct gcaggagcct ggtgagccag
     2221 ccgccttcat cagagaagcc catggacagc atctgggaga gggactcaat cagccgcggg
     2281 tcagcctctg gcgggagatg tgggtacaag gcagcttcct tcagccctgt gggtccctcc
     2341 tgggaggggt ccagagagct tggcccttcg gattctggca tctgtaggga ctggagttca
     2401 cctgtagacg ggtccacttc ttttgaagac agatgggtcc agtcatcatc tcctcctgaa
     2461 cagttatccg actccatctg ttcctcaggg cgcccctcgg actccaaggc gatctttctc
     2521 atctgctccg ccagagactg cgtggcgccc tcaacattcc cacccggctt gctgggatca
     2581 gagcagcagc tgcttggctg tgagctgctc ttctcctctg tgctggaact ctctggagag
     2641 acgggggtca ggcggcttct tttccctccg tgctccacat cgatatcaac ttcaatgccc
     2701 agagggctaa gggcagctgc cacactctcc ccaacgttct tcaggaaatt cacactcgga
     2761 tcctccgatg gaccagaagc tgattctgcc gtggggccag ggcgggcctc ccctgcacga
     2821 ggaggacgtg ggctccagtt tcctggtgga cccatttccc atcctggcca cccgaagtgt
     2881 ccgtgtttca ccttccggag ccagcggctg tgcgagaagc cctcagacag gtgcccgaag
     2941 gggctgggga atgcgagctt ggtgtgcccc cggtgcaagc cctttccctc gcagacgcta
     3001 cacaagtcgt agtctgggca gacgctgcac ttgtagcggg ttcctaccac aggcccattg
     3061 cagccatcgc agatcacatt ggggtgcacc atgttgcggg gcgcctcctg agcacacggt
     3121 gggcggtggt cccgccggca ctcttttttc tctttaatgt agattcggaa gatgtcatcc
     3181 ttcacgtagg acatggccat tgtcaattcc tcgtcactgg aaaaggcaac caagtccccg
     3241 tcctcatcgc ggtagtgcgc ctggaagccg ccaggccgca gcgcggggaa cagggcggcc
     3301 acccggctca gcagccgctc gcagggtccc ggacccgccg cagcctcggc ttccgcctca
     3361 ggctcggggc tgcagcagaa gctgaagcgg cgaatctcgc gcgccgcgtc ctccttgccc
     3421 agaaggtagg ccttcacggt gagcgacgcc atagaattcc accacactgg actagtggat
     3481 ccgagctcgg taccaagctt aagtttaaac gctagagtcc ggaggctgga tcggtcccgg
     3541 tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact
     3601 aaacgagctc gtcgacgatc tctatcactg atagggagat ctctatcact gatagggaga
     3661 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     3721 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     3781 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     3841 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     3901 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     3961 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     4021 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     4081 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     4141 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     4201 cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc
     4261 cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg
     4321 gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac
     4381 acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     4441 gtgcaccata ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg
     4501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4561 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4621 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4681 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4741 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4801 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4861 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4921 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4981 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5041 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5101 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5161 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5221 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5281 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5341 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5401 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5461 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5521 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5581 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5761 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
