LOCUS       40924_1204        4227 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmubiSINC-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Grant TN, De La Torre CM, Zhang N, Finer JJ.
  TITLE     Synthetic introns help identify sequences in the 5' UTR intron of 
            the Glycine max polyubiquitin (Gmubi) promoter that give increased 
            promoter activity
  JOURNAL   Planta (2017) In press
  PUBMED    28070655
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Finer J, Grant T, De La Torre C, Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4227)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4227)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Planta 
            (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-SEP-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      412..973
                     /label=GmubiSINC synthetic promoter
                     /note="GmubiSINC synthetic promoter"
                     /regulatory_class="promoter"
     CDS             974..1690
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      1712..1964
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2006..2022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2030..2046)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2054..2084)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2099..2120)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2408..2996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3170..4027)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4028..4132)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc gggcccaata
      421 taacaacgac gtcgtaacag ataaagcgaa gcttgaaggt gcatgtgact ccgtcaagat
      481 tacgaaaccg ccaactacca cgcaaattgc aattctcaat ttcctagaag gactctccga
      541 aaatgcatcc aataccaaat attacccgtg tcataggcac caagtgacac catacatgaa
      601 cacgcgtcac aatatgactg gagaagggtt ccacacctta tgctataaaa cgccccacac
      661 ccctcctcct tccttcgcag ttcaattcca atatattcca ttctctctgt gtatttccct
      721 acctctccct tcaaggttag tcgatttctt ctgtttttct tcttcgttct ttccatgaat
      781 tgtgtatgtt ctttgatcag ctagcggtac cactagttct agagtcgaca gatctgttag
      841 caactgcctt gtttgttgat tctattgccg tggattaggg ttttttttca cgagattgct
      901 tcagatccgt acttaagatt acgtaatgga ttttgattct gatttatctg tgattgttga
      961 ctcgacagga tccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1021 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1081 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1141 gccctggccc accctcgtga ccaccttcac ctacggcgtg cagtgcttca gccgctaccc
     1201 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1261 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1321 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1381 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     1441 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     1501 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1561 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     1621 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactcacg gcatggacga
     1681 gctgtacaag taaagcggcc gcccggctgc agatcgttca aacatttggc aataaagttt
     1741 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     1801 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     1861 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     1921 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatccgatga taagctgtca
     1981 aacatgagaa ttcggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2041 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2101 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2161 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2821 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     2881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4141 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     4201 ataggcgtat cacgaggccc tttcgtc
//
