LOCUS       40924_1199        5175 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM9-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5175)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5175)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1892
                     /label=GmScreamM9 promoter
                     /note="GmScreamM9 promoter"
                     /regulatory_class="promoter"
     CDS             1895..2611
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2647..2899
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2954..2970)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2978..2994)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3002..3032)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3047..3068)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3356..3944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4118..4975)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4976..5080)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttagtgtg atgctcctta
      421 gtcttgttga tctttcttaa caaaatttga cacagatctt taagacatta aaagtatcat
      481 cttagagttt tcaacttaca aaagtattca gtgataaaat gtaactttga tgcatcttta
      541 tttggcgatg cagacatctt tggagtaaca actgttataa gaaatcatca gggtcatttc
      601 caatcagcaa agacaacctg gtttataggc aatccgctac cacaggaagc agaagcaata
      661 gctttccaaa caactatcaa ttggatatcc aaacaaaatc tccatcatgc cctactcaga
      721 ttgcaaccat tttgtagact gcatgaattc tcaaatcttc aacaactatg aatttggagc
      781 catccttgaa gtttgttaag agaaggtttt caacatttca ataaactgtc ctctcaagct
      841 gtcaattatc tcacaagaaa atttagattt tatgctagtc aacatgtttt tgatttaatt
      901 cctacttcta ttaactcttt aattatgaat gaaatcctat gagcatgatt taataataaa
      961 ataaaacaaa taaatttaca aaacgtatta tttcataaaa atacaaaatt atactgttac
     1021 aaaattttgt ctcgtgcatc gcactccatg ttttgtttgt ctatattttt gttttatatt
     1081 attttagagt tagatttagt tgtaaatcat tatcatcgtt gaaaagttat tttttatatt
     1141 aaccattgtg attctggggt gattttcaat acatgctata aggataaaat agtaaaaaaa
     1201 ttaaatttaa atacattttt ggttcttgtt atttaaattt tttattttta tttttttttc
     1261 tgtacttttt tgtttttgtt ttagtcatta taaaatatgt ttgttttgat ttttgttctt
     1321 aaaatacttt atttaaataa cactttaaac aagaaaaatt atctaaaata atttaagaat
     1381 aaataataaa taatcacatt ttataagaat ttaaatgaac aaaaaattac agaaacaaaa
     1441 ataacaaaat tgaaattaca aaaaataaaa aatatgttta aaccaagaat ttacatacat
     1501 taaaaataga tatccccttt attataagca tatattatta cattataaaa aaacttatgt
     1561 acatcccctt catctatgca atggatgagc aaaatactaa taataaatat tttctcaaaa
     1621 acatcttcat ttttctttcg gccacgcttc cctagattca aattcaaact tgtaactgtc
     1681 acaggctcat catattcaga taagcattgg gcttgctagg cccaacggac ccattaatcc
     1741 ataggccgag aaacaccaca aaaaggaata agaacggtcc aaatccttat tctataagag
     1801 acccttttcg caatcacaat ttttctcata cggcatttgt cggcggtgac gaaaggaaag
     1861 aagaagaaga agcgttcttt cggagagttc gaccatggtg agcaagggcg aggagctgtt
     1921 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1981 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     2041 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttca cctacggcgt
     2101 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     2161 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2221 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2281 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     2341 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2401 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2461 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     2521 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2581 gatcactcac ggcatggacg agctgtacaa gtaaagcggc cgcccggctg cactgaggcc
     2641 tcagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     2701 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     2761 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     2821 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     2881 tcatctatgt tactagatcc gatgataagc tgtcaaacat gagaattaga tctcaattga
     2941 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3001 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3061 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3121 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3181 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3241 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3301 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3361 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3421 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3481 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3541 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3601 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3661 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3721 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3781 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3841 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3901 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3961 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4021 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4081 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4141 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4201 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4261 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4321 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4381 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4441 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4501 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4561 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4621 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4681 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4741 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4801 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4861 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4921 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4981 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5041 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5101 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     5161 cgaggccctt tcgtc
//
