LOCUS 40924_1199 5175 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector GmScreamM9-pFLEV, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5175) AUTHORS Zhang N, McHale LK, Finer JJ. TITLE Isolation and characterization of 'GmScream' promoters that regulate highly expressing soybean (Glycine max Merr.) genes JOURNAL Plant Sci. 241, 189-198 (2015) PUBMED 26706070 REFERENCE 2 (bases 1 to 5175) AUTHORS Zhang N. TITLE Direct Submission JOURNAL Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA REFERENCE 3 (bases 1 to 5175) TITLE Direct Submission REFERENCE 4 (bases 1 to 5175) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 241, 189-198 (2015)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5175 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 405..1892 /label=GmScreamM9 promoter /note="GmScreamM9 promoter" /regulatory_class="promoter" CDS 1895..2611 /label=EGFP /note="enhanced GFP" terminator 2647..2899 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(2954..2970) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(2978..2994) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3002..3032) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3047..3068) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3356..3944) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4118..4975) /label=AmpR /note="beta-lactamase" promoter complement(4976..5080) /label=AmpR promoter ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttagtgtg atgctcctta 421 gtcttgttga tctttcttaa caaaatttga cacagatctt taagacatta aaagtatcat 481 cttagagttt tcaacttaca aaagtattca gtgataaaat gtaactttga tgcatcttta 541 tttggcgatg cagacatctt tggagtaaca actgttataa gaaatcatca gggtcatttc 601 caatcagcaa agacaacctg gtttataggc aatccgctac cacaggaagc agaagcaata 661 gctttccaaa caactatcaa ttggatatcc aaacaaaatc tccatcatgc cctactcaga 721 ttgcaaccat tttgtagact gcatgaattc tcaaatcttc aacaactatg aatttggagc 781 catccttgaa gtttgttaag agaaggtttt caacatttca ataaactgtc ctctcaagct 841 gtcaattatc tcacaagaaa atttagattt tatgctagtc aacatgtttt tgatttaatt 901 cctacttcta ttaactcttt aattatgaat gaaatcctat gagcatgatt taataataaa 961 ataaaacaaa taaatttaca aaacgtatta tttcataaaa atacaaaatt atactgttac 1021 aaaattttgt ctcgtgcatc gcactccatg ttttgtttgt ctatattttt gttttatatt 1081 attttagagt tagatttagt tgtaaatcat tatcatcgtt gaaaagttat tttttatatt 1141 aaccattgtg attctggggt gattttcaat acatgctata aggataaaat agtaaaaaaa 1201 ttaaatttaa atacattttt ggttcttgtt atttaaattt tttattttta tttttttttc 1261 tgtacttttt tgtttttgtt ttagtcatta taaaatatgt ttgttttgat ttttgttctt 1321 aaaatacttt atttaaataa cactttaaac aagaaaaatt atctaaaata atttaagaat 1381 aaataataaa taatcacatt ttataagaat ttaaatgaac aaaaaattac agaaacaaaa 1441 ataacaaaat tgaaattaca aaaaataaaa aatatgttta aaccaagaat ttacatacat 1501 taaaaataga tatccccttt attataagca tatattatta cattataaaa aaacttatgt 1561 acatcccctt catctatgca atggatgagc aaaatactaa taataaatat tttctcaaaa 1621 acatcttcat ttttctttcg gccacgcttc cctagattca aattcaaact tgtaactgtc 1681 acaggctcat catattcaga taagcattgg gcttgctagg cccaacggac ccattaatcc 1741 ataggccgag aaacaccaca aaaaggaata agaacggtcc aaatccttat tctataagag 1801 acccttttcg caatcacaat ttttctcata cggcatttgt cggcggtgac gaaaggaaag 1861 aagaagaaga agcgttcttt cggagagttc gaccatggtg agcaagggcg aggagctgtt 1921 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 1981 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 2041 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttca cctacggcgt 2101 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 2161 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 2221 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 2281 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 2341 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 2401 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 2461 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 2521 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 2581 gatcactcac ggcatggacg agctgtacaa gtaaagcggc cgcccggctg cactgaggcc 2641 tcagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt 2701 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg 2761 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt 2821 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg 2881 tcatctatgt tactagatcc gatgataagc tgtcaaacat gagaattaga tctcaattga 2941 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 3001 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 3061 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 3121 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 3181 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 3241 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 3301 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 3361 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3421 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3481 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3541 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3601 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3661 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3721 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 3781 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 3841 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 3901 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 3961 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 4021 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 4081 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 4141 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 4201 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 4261 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 4321 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 4381 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 4441 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 4501 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 4561 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 4621 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 4681 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 4741 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 4801 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 4861 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 4921 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 4981 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 5041 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 5101 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 5161 cgaggccctt tcgtc //