LOCUS 40924_1194 4693 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector GmScreamM8S-pFLEV, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4693) AUTHORS Zhang N, McHale LK, Finer JJ. TITLE Isolation and characterization of 'GmScream' promoters that regulate highly expressing soybean (Glycine max Merr.) genes JOURNAL Plant Sci. 241, 189-198 (2015) PUBMED 26706070 REFERENCE 2 (bases 1 to 4693) AUTHORS Zhang N. TITLE Direct Submission JOURNAL Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA REFERENCE 3 (bases 1 to 4693) TITLE Direct Submission REFERENCE 4 (bases 1 to 4693) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 241, 189-198 (2015)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4693 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 405..1410 /label=GmScreamM8S promoter /note="GmScreamM8S promoter" /regulatory_class="promoter" CDS 1413..2129 /label=EGFP /note="enhanced GFP" terminator 2165..2417 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(2472..2488) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(2496..2512) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2520..2550) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(2565..2586) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(2874..3462) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3636..4493) /label=AmpR /note="beta-lactamase" promoter complement(4494..4598) /label=AmpR promoter ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctacga gacgtgagaa 421 gcacgcgctt tagcgagcgt ttcaattgtc gctacgaaag cagagaagga tacaaacgga 481 actagggtaa attagtaagg gtaatttcgt aaacagaaga aaagagttgt agctataaat 541 aaaccctcta accctcgtcg cattacttct cttcacacct ttgttcactc ttcttctctt 601 gcggctaggg ttttagcgca gcttcttcta ggttcgttat ctaccaccgt tctatggatt 661 ttattccttc tattcgtgtt tattctattg gtttatgttg cttgcaatat gttttttctg 721 aatctgtcgt cgttgtcttc aattttatcc atgtttcaga gatcaatttt gtttgtgtag 781 tatgtgctta ttcttcttct tttcgttcga gttgttaata acggtgctat ggtgttttca 841 aaagtgtttt ttttattact tttgatttaa agtttttttg gtaaggcttt tatttgcttg 901 ttatattcaa atctttggat ccagatctta tataagtttt tggttcaaga aagtttttgg 961 ttactgatga atagatctat taactgttac tttaatcgat tcaagctaaa gttttttggt 1021 tactgatgaa tagatctatt atctgttact tttaatcggt tcaagctcaa gttttttggt 1081 tactgatgaa tagatctata tacgtcacag tgtgctaaac atgcccttgt tttatctcga 1141 tcttatgtat gggagtgcca taaattttgt tatgtctatt tttttatctg ttggaatcat 1201 actgagtttg atgcgttacg attgagcata cctatttttg ggcttgttgt atggtgggta 1261 tttagatctt aatcttttta tgcttatgaa aggttttgta atgacaaagg tcttaatgtt 1321 gttaaacttt tatttttact ttatatggtg tgttgatgtg ttatggtttt gacaactttt 1381 tttttttctg gatttttgca gatttaagga ccatggtgag caagggcgag gagctgttca 1441 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 1501 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 1561 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc 1621 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 1681 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 1741 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 1801 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 1861 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 1921 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 1981 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 2041 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 2101 tcactcacgg catggacgag ctgtacaagt aaagcggccg cccggctgca ctgaggcctc 2161 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct 2221 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta 2281 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta 2341 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc 2401 atctatgtta ctagatccga tgataagctg tcaaacatga gaattagatc tcaattgaat 2461 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 2521 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 2581 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 2641 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 2701 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 2761 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 2821 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 2881 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 2941 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 3001 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 3061 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 3121 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 3181 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 3241 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 3301 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 3361 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 3421 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 3481 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 3541 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 3601 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 3661 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 3721 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 3781 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 3841 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 3901 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 3961 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 4021 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 4081 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 4141 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 4201 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 4261 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 4321 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 4381 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 4441 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 4501 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 4561 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 4621 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 4681 aggccctttc gtc //