LOCUS       40924_1194        4693 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM8S-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4693)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4693)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1410
                     /label=GmScreamM8S promoter
                     /note="GmScreamM8S promoter"
                     /regulatory_class="promoter"
     CDS             1413..2129
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2165..2417
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2472..2488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2496..2512)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2520..2550)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2565..2586)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2874..3462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3636..4493)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4494..4598)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctacga gacgtgagaa
      421 gcacgcgctt tagcgagcgt ttcaattgtc gctacgaaag cagagaagga tacaaacgga
      481 actagggtaa attagtaagg gtaatttcgt aaacagaaga aaagagttgt agctataaat
      541 aaaccctcta accctcgtcg cattacttct cttcacacct ttgttcactc ttcttctctt
      601 gcggctaggg ttttagcgca gcttcttcta ggttcgttat ctaccaccgt tctatggatt
      661 ttattccttc tattcgtgtt tattctattg gtttatgttg cttgcaatat gttttttctg
      721 aatctgtcgt cgttgtcttc aattttatcc atgtttcaga gatcaatttt gtttgtgtag
      781 tatgtgctta ttcttcttct tttcgttcga gttgttaata acggtgctat ggtgttttca
      841 aaagtgtttt ttttattact tttgatttaa agtttttttg gtaaggcttt tatttgcttg
      901 ttatattcaa atctttggat ccagatctta tataagtttt tggttcaaga aagtttttgg
      961 ttactgatga atagatctat taactgttac tttaatcgat tcaagctaaa gttttttggt
     1021 tactgatgaa tagatctatt atctgttact tttaatcggt tcaagctcaa gttttttggt
     1081 tactgatgaa tagatctata tacgtcacag tgtgctaaac atgcccttgt tttatctcga
     1141 tcttatgtat gggagtgcca taaattttgt tatgtctatt tttttatctg ttggaatcat
     1201 actgagtttg atgcgttacg attgagcata cctatttttg ggcttgttgt atggtgggta
     1261 tttagatctt aatcttttta tgcttatgaa aggttttgta atgacaaagg tcttaatgtt
     1321 gttaaacttt tatttttact ttatatggtg tgttgatgtg ttatggtttt gacaactttt
     1381 tttttttctg gatttttgca gatttaagga ccatggtgag caagggcgag gagctgttca
     1441 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1501 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     1561 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc
     1621 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1681 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1741 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1801 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1861 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     1921 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1981 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     2041 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2101 tcactcacgg catggacgag ctgtacaagt aaagcggccg cccggctgca ctgaggcctc
     2161 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     2221 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     2281 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     2341 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     2401 atctatgtta ctagatccga tgataagctg tcaaacatga gaattagatc tcaattgaat
     2461 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     2521 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2581 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2641 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2701 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2761 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2821 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2881 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2941 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3001 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3061 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3121 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3181 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3241 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3301 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3361 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3421 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3481 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3541 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3601 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3661 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3721 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3781 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3841 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3901 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3961 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4021 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4081 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4141 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4201 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4261 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4321 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4381 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4441 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4501 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4561 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4621 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4681 aggccctttc gtc
//
