LOCUS       40924_1189        4899 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM8M-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4899)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4899)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1616
                     /label=GmScreamM8M promoter
                     /note="GmScreamM8M promoter"
                     /regulatory_class="promoter"
     CDS             1619..2335
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2371..2623
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2678..2694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2702..2718)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2726..2756)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2771..2792)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3080..3668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3842..4699)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4700..4804)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttaatact atcgagtaat
      421 ggccgcgcta gggtttttga gaaaaaatct tcccacgcac tcaactgcac tgtacggcgt
      481 cgttttcaca gccgcataat agaagccgcg ttccccaacc cttcctcaca acattctcgg
      541 accctccagc accgtcaccc aaacaaatat ccacgcggta gtaggcgcgt gaaacaaact
      601 ctaatccgaa ctacgagacg tgagaagcac gcgctttagc gagcgtttca attgtcgcta
      661 cgaaagcaga gaaggataca aacggaacta gggtaaatta gtaagggtaa tttcgtaaac
      721 agaagaaaag agttgtagct ataaataaac cctctaaccc tcgtcgcatt acttctcttc
      781 acacctttgt tcactcttct tctcttgcgg ctagggtttt agcgcagctt cttctaggtt
      841 cgttatctac caccgttcta tggattttat tccttctatt cgtgtttatt ctattggttt
      901 atgttgcttg caatatgttt tttctgaatc tgtcgtcgtt gtcttcaatt ttatccatgt
      961 ttcagagatc aattttgttt gtgtagtatg tgcttattct tcttcttttc gttcgagttg
     1021 ttaataacgg tgctatggtg ttttcaaaag tgtttttttt attacttttg atttaaagtt
     1081 tttttggtaa ggcttttatt tgcttgttat attcaaatct ttggatccag atcttatata
     1141 agtttttggt tcaagaaagt ttttggttac tgatgaatag atctattaac tgttacttta
     1201 atcgattcaa gctaaagttt tttggttact gatgaataga tctattatct gttactttta
     1261 atcggttcaa gctcaagttt tttggttact gatgaataga tctatatacg tcacagtgtg
     1321 ctaaacatgc ccttgtttta tctcgatctt atgtatggga gtgccataaa ttttgttatg
     1381 tctatttttt tatctgttgg aatcatactg agtttgatgc gttacgattg agcataccta
     1441 tttttgggct tgttgtatgg tgggtattta gatcttaatc tttttatgct tatgaaaggt
     1501 tttgtaatga caaaggtctt aatgttgtta aacttttatt tttactttat atggtgtgtt
     1561 gatgtgttat ggttttgaca actttttttt tttctggatt tttgcagatt taaggaccat
     1621 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1681 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1741 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1801 cgtgaccacc ttcacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1861 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1921 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1981 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2041 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2101 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2161 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2221 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2281 gctggagttc gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaagtaaag
     2341 cggccgcccg gctgcactga ggcctcagca gatcgttcaa acatttggca ataaagtttc
     2401 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     2461 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     2521 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     2581 taggataaat tatcgcgcgc ggtgtcatct atgttactag atccgatgat aagctgtcaa
     2641 acatgagaat tagatctcaa ttgaattcgt aatcatggtc atagctgttt cctgtgtgaa
     2701 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2761 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     2821 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2881 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2941 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3061 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3481 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     3541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3841 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3901 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3961 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4021 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4081 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4141 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4201 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4261 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4321 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4381 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4441 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4501 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4561 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4621 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4681 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4741 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4801 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     4861 taacctataa aaataggcgt atcacgaggc cctttcgtc
//
