LOCUS       40924_1174        5261 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM8-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5261)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5261)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1978
                     /label=GmScreamM8 promoter
                     /note="GmScreamM8 promoter"
                     /regulatory_class="promoter"
     CDS             1981..2697
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2733..2985
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3040..3056)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3064..3080)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3088..3118)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3133..3154)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3442..4030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4204..5061)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5062..5166)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttttacgt gtgctgtgag
      421 acattatcat caattgtgtt gtatatgata tatagatata gatatataaa tatatagatt
      481 gagtgatata atatatttaa aatataaatt atatatatgt tttaatatat ttttgcatat
      541 atatatatat ttgtaaaaac tagaagtatt ttttcatgag ataattatta tcgagttgaa
      601 taagtctatt atttgtgaga gccaaccata tttatatatg tgattaaatt ttatctttgt
      661 gaaattaaaa ataataaaaa ataccttaaa atcataataa tagaaaaact tatatttata
      721 atttaccatt atacttaaaa aaaattaaat aaatattata aatataaata ctatcgagta
      781 atggccgcgc tagggttttt gagaaaaaat cttcccacgc actcaactgc actgtacggc
      841 gtcgttttca cagccgcata atagaagccg cgttccccaa cccttcctca caacattctc
      901 ggaccctcca gcaccgtcac ccaaacaaat atccacgcgg tagtaggcgc gtgaaacaaa
      961 ctctaatccg aactacgaga cgtgagaagc acgcgcttta gcgagcgttt caattgtcgc
     1021 tacgaaagca gagaaggata caaacggaac tagggtaaat tagtaagggt aatttcgtaa
     1081 acagaagaaa agagttgtag ctataaataa accctctaac cctcgtcgca ttacttctct
     1141 tcacaccttt gttcactctt cttctcttgc ggctagggtt ttagcgcagc ttcttctagg
     1201 ttcgttatct accaccgttc tatggatttt attccttcta ttcgtgttta ttctattggt
     1261 ttatgttgct tgcaatatgt tttttctgaa tctgtcgtcg ttgtcttcaa ttttatccat
     1321 gtttcagaga tcaattttgt ttgtgtagta tgtgcttatt cttcttcttt tcgttcgagt
     1381 tgttaataac ggtgctatgg tgttttcaaa agtgtttttt ttattacttt tgatttaaag
     1441 tttttttggt aaggctttta tttgcttgtt atattcaaat ctttggatcc agatcttata
     1501 taagtttttg gttcaagaaa gtttttggtt actgatgaat agatctatta actgttactt
     1561 taatcgattc aagctaaagt tttttggtta ctgatgaata gatctattat ctgttacttt
     1621 taatcggttc aagctcaagt tttttggtta ctgatgaata gatctatata cgtcacagtg
     1681 tgctaaacat gcccttgttt tatctcgatc ttatgtatgg gagtgccata aattttgtta
     1741 tgtctatttt tttatctgtt ggaatcatac tgagtttgat gcgttacgat tgagcatacc
     1801 tatttttggg cttgttgtat ggtgggtatt tagatcttaa tctttttatg cttatgaaag
     1861 gttttgtaat gacaaaggtc ttaatgttgt taaactttta tttttacttt atatggtgtg
     1921 ttgatgtgtt atggttttga caactttttt tttttctgga tttttgcaga tttaaggacc
     1981 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2041 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     2101 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2161 ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2221 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2281 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2341 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2401 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2461 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2521 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2581 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2641 ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagtaa
     2701 agcggccgcc cggctgcact gaggcctcag cagatcgttc aaacatttgg caataaagtt
     2761 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     2821 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     2881 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     2941 actaggataa attatcgcgc gcggtgtcat ctatgttact agatccgatg ataagctgtc
     3001 aaacatgaga attagatctc aattgaattc gtaatcatgg tcatagctgt ttcctgtgtg
     3061 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3121 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3181 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3241 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3301 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3841 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     3901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4201 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4261 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4321 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4381 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4441 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4501 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4561 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4621 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4681 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4741 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4801 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4861 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4921 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4981 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5041 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5101 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5161 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     5221 attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
