LOCUS 40924_1174 5261 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector GmScreamM8-pFLEV, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5261) AUTHORS Zhang N, McHale LK, Finer JJ. TITLE Isolation and characterization of 'GmScream' promoters that regulate highly expressing soybean (Glycine max Merr.) genes JOURNAL Plant Sci. 241, 189-198 (2015) PUBMED 26706070 REFERENCE 2 (bases 1 to 5261) AUTHORS Zhang N. TITLE Direct Submission JOURNAL Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA REFERENCE 3 (bases 1 to 5261) TITLE Direct Submission REFERENCE 4 (bases 1 to 5261) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 241, 189-198 (2015)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5261 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 405..1978 /label=GmScreamM8 promoter /note="GmScreamM8 promoter" /regulatory_class="promoter" CDS 1981..2697 /label=EGFP /note="enhanced GFP" terminator 2733..2985 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(3040..3056) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3064..3080) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3088..3118) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3133..3154) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3442..4030) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4204..5061) /label=AmpR /note="beta-lactamase" promoter complement(5062..5166) /label=AmpR promoter ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttttacgt gtgctgtgag 421 acattatcat caattgtgtt gtatatgata tatagatata gatatataaa tatatagatt 481 gagtgatata atatatttaa aatataaatt atatatatgt tttaatatat ttttgcatat 541 atatatatat ttgtaaaaac tagaagtatt ttttcatgag ataattatta tcgagttgaa 601 taagtctatt atttgtgaga gccaaccata tttatatatg tgattaaatt ttatctttgt 661 gaaattaaaa ataataaaaa ataccttaaa atcataataa tagaaaaact tatatttata 721 atttaccatt atacttaaaa aaaattaaat aaatattata aatataaata ctatcgagta 781 atggccgcgc tagggttttt gagaaaaaat cttcccacgc actcaactgc actgtacggc 841 gtcgttttca cagccgcata atagaagccg cgttccccaa cccttcctca caacattctc 901 ggaccctcca gcaccgtcac ccaaacaaat atccacgcgg tagtaggcgc gtgaaacaaa 961 ctctaatccg aactacgaga cgtgagaagc acgcgcttta gcgagcgttt caattgtcgc 1021 tacgaaagca gagaaggata caaacggaac tagggtaaat tagtaagggt aatttcgtaa 1081 acagaagaaa agagttgtag ctataaataa accctctaac cctcgtcgca ttacttctct 1141 tcacaccttt gttcactctt cttctcttgc ggctagggtt ttagcgcagc ttcttctagg 1201 ttcgttatct accaccgttc tatggatttt attccttcta ttcgtgttta ttctattggt 1261 ttatgttgct tgcaatatgt tttttctgaa tctgtcgtcg ttgtcttcaa ttttatccat 1321 gtttcagaga tcaattttgt ttgtgtagta tgtgcttatt cttcttcttt tcgttcgagt 1381 tgttaataac ggtgctatgg tgttttcaaa agtgtttttt ttattacttt tgatttaaag 1441 tttttttggt aaggctttta tttgcttgtt atattcaaat ctttggatcc agatcttata 1501 taagtttttg gttcaagaaa gtttttggtt actgatgaat agatctatta actgttactt 1561 taatcgattc aagctaaagt tttttggtta ctgatgaata gatctattat ctgttacttt 1621 taatcggttc aagctcaagt tttttggtta ctgatgaata gatctatata cgtcacagtg 1681 tgctaaacat gcccttgttt tatctcgatc ttatgtatgg gagtgccata aattttgtta 1741 tgtctatttt tttatctgtt ggaatcatac tgagtttgat gcgttacgat tgagcatacc 1801 tatttttggg cttgttgtat ggtgggtatt tagatcttaa tctttttatg cttatgaaag 1861 gttttgtaat gacaaaggtc ttaatgttgt taaactttta tttttacttt atatggtgtg 1921 ttgatgtgtt atggttttga caactttttt tttttctgga tttttgcaga tttaaggacc 1981 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 2041 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 2101 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 2161 ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 2221 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 2281 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 2341 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 2401 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 2461 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 2521 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 2581 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 2641 ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagtaa 2701 agcggccgcc cggctgcact gaggcctcag cagatcgttc aaacatttgg caataaagtt 2761 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt 2821 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta 2881 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa 2941 actaggataa attatcgcgc gcggtgtcat ctatgttact agatccgatg ataagctgtc 3001 aaacatgaga attagatctc aattgaattc gtaatcatgg tcatagctgt ttcctgtgtg 3061 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 3121 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 3181 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 3241 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 3301 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 3361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 3421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 3481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 3541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 3601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 3661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 3721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 3781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 3841 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 3901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 3961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 4021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 4081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 4141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 4201 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 4261 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 4321 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 4381 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 4441 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 4501 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 4561 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 4621 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 4681 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 4741 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 4801 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 4861 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 4921 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 4981 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 5041 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 5101 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 5161 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 5221 attaacctat aaaaataggc gtatcacgag gccctttcgt c //