LOCUS       40924_1169        5095 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM7-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5095)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5095)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1812
                     /label=GmScreamM7 promoter
                     /note="GmScreamM7 promoter"
                     /regulatory_class="promoter"
     CDS             1815..2531
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2567..2819
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2874..2890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2898..2914)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2922..2952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2967..2988)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3276..3864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4038..4895)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4896..5000)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctagac tcatcttctc
      421 cttgaagtgg cgtctcctct ctctctccct ttctccattc cgctgccgtt catcttccaa
      481 gaagcaaagg aatccattga tgaagaagat cctaggccta caagctccaa tggagcttgc
      541 atcacactcc ctacccacga cccacgaccc ctacgcgcac gacctcaatc tctgaggctc
      601 ttcgtgtgtg actctctatc ttcagatatg ggttcttggg tttggggatt tttggatttg
      661 ttgggtttct aggtttggga tggagaccgg ggtctatggt gtcggggtgg ttgttgttcc
      721 aatgatgtta agccaacaat ggaaacacga tttcattttt ggaattacat aaaaactcaa
      781 ccacgtggat agtgacaata atacttttgg tggtgccaat agtagcttcc caagacttat
      841 ttatttttat gtgttatttt tttttggcct tggcccgttg attagaaaaa aataatacga
      901 aggttaaaaa cgtcattttt gaattattag gccctatata aatttcggga ggggctaaat
      961 ttttctctct cgtctcgtgg aagtgtaagc aagcaaaacc ctagcggcgc ctgctttctt
     1021 tgttcgtgtt ttgcatcagg tgcgaactca ccctattgat ttctatccac agctgatcct
     1081 tcttctctaa ttaatttatt attatttttc atgtattgtt ctcgcttcac tgcttcttat
     1141 ttggctccgt catttgaaat tgttttcttt taattcgagt ttcaaatttt caatatctgt
     1201 tactgtgttt atgatttcaa tttcatggtg gaattattat tgctttctac gtgacgttga
     1261 atttgtactg tatgtgttga tcttttgttc ttaatttggg tcgcatagtg tttgtggctt
     1321 gtttggtgaa gtaacgtaga cccgagtgtt ctaacaaatg atatgttgga ttctttcatt
     1381 agcatgcttt ttggaatttt tgacctttgg aagtcgtata ctgttcagca tcactaaatt
     1441 tagtcctccc tcaacaaaag aatctggtgt aggatgcatc ctatcaaatg aaacaaggtt
     1501 tatgctgaag tacaagaaga ctgatctgtt attttgatgt tattgctgga cttggttttc
     1561 ttttttcctt ttatgtgatg tgtgccatgt ccatatttgt atttttaccc tatcagttgt
     1621 ttggttcgtg tgaaactgtg aatgcttttg ttatttcctg ttactttgga tgtcttcaat
     1681 ttaaggtttt tagtcttctg gatttcgaag gtgatacata tttacctgtc acggtgacca
     1741 attagctgtg atgcattaaa cccaattgtt ttccacattc atctcataaa aactgttgat
     1801 tgtgtttgag caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1861 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1921 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1981 tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc agccgctacc
     2041 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2101 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2161 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2221 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     2281 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     2341 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2401 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     2461 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactcac ggcatggacg
     2521 agctgtacaa gtaaagcggc cgcccggctg cactgaggcc tcagcagatc gttcaaacat
     2581 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     2641 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     2701 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     2761 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcc
     2821 gatgataagc tgtcaaacat gagaattaga tctcaattga attcgtaatc atggtcatag
     2881 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2941 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     3001 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     3061 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4321 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     5041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
