LOCUS       40924_1164        5016 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM6-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5016)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5016)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1733
                     /label=GmScreamM6 promoter
                     /note="GmScreamM6 promoter"
                     /regulatory_class="promoter"
     CDS             1736..2452
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2488..2740
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2795..2811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2819..2835)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2843..2873)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2888..2909)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3197..3785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3959..4816)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4817..4921)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttcgtctc ttaatctcga
      421 tcgtgtgagt aaaatggaac atgtataaat gttacatgtt atcctcacta tcattcgcta
      481 tgactaatag tctaatacta atactaataa tgataataat gtttgttttt aaaaatagta
      541 ataataaaac aagttcacaa gttaggtcaa tgagtgcata aatgaaaacc tgtgacccaa
      601 atccaaagtc aacgaatttg aggattttgc ttcttgcatt ttttttttct tcctgcactt
      661 ccaaaaatac tcgaatggac acaactaccc ttacaagcac caaccaaaat tcaacacaaa
      721 tcgataacgc ctactcccac caaaatggaa accattttca ccaacaacaa caaggttcga
      781 aaggtagtaa caaggaagag aaaaggaaaa gggttgcaat ggagatgtcc gaagtagaag
      841 aataaaacac tatcaatgta acccactact ttgttcttgc taacatgcat caacaaacta
      901 aactaaatgc aatgtacaat acatggcaaa aaataatgtc aaatcataag aaattcaagc
      961 acaaatattg ccctaaaaat taaattcaac aaagaagttt ctatattgca gtaataattt
     1021 ttcactttgc cccctcctct tagactatgc gcggtggacc aaagctaaag ttagaagggc
     1081 taggcaagac aaaagcatta tgttgaggtt ccaaattagg ataaaaggaa aagtgagaag
     1141 gaagcaagga gtgtcaccct attttgaagc agctattatg gaatgtgaaa attagattaa
     1201 gaaatatgta ttctggaata ggtttataga tttcttatct ttattcacta ataactattt
     1261 caaaatgtaa aaattatata tttatctaaa cccactagat actcaatcca aactaattga
     1321 cttgattaag acggaatttg aattcgtggg aaattcatac taggtttggg gcaaggggtg
     1381 gaataagaag aaaaaaatgg ataaggatgt catttggtgt gggtatatag ccaaccagaa
     1441 aagggctggg tatttttgag tgtgtgagag agcgagcgag cgagcagttt caaagagagg
     1501 atttgggatt gggaaggcga gggattgcgc tgcgtctctt cctcttctct ttcgattcat
     1561 ccctcattca taatcttcca atccaacaac ctcagcctca cactcacaca tacacacgtc
     1621 tctctctctc tactcttttt tcctctctat ttcccttttc actttctctt tcatttctag
     1681 ggttccgttc tgatccctct ttccctttca gatctgaacg cgtatcgtcc ctcccatggt
     1741 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1801 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1861 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1921 gaccaccttc acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1981 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2041 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2101 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     2161 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     2221 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     2281 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     2341 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2401 ggagttcgtg accgccgccg ggatcactca cggcatggac gagctgtaca agtaaagcgg
     2461 ccgcccggct gcactgaggc ctcagcagat cgttcaaaca tttggcaata aagtttctta
     2521 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     2581 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     2641 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     2701 gataaattat cgcgcgcggt gtcatctatg ttactagatc cgatgataag ctgtcaaaca
     2761 tgagaattag atctcaattg aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     2821 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2881 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2941 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3001 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3601 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4921 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     4981 cctataaaaa taggcgtatc acgaggccct ttcgtc
//
