LOCUS       40924_1149        5259 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM3-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5259)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5259)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1976
                     /label=GmScreamM3 promoter
                     /note="GmScreamM3 promoter"
                     /regulatory_class="promoter"
     CDS             1979..2695
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2731..2983
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3038..3054)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3062..3078)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3086..3116)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3131..3152)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3440..4028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4202..5059)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5060..5164)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttcacctt ctcctcagca
      421 tgtcattcat tccaccttca acattcccat caccaacctt tccaaacccc ataaactcct
      481 tcagaaccac actagtcctc tcaccgcgag acaaaaaccc caccagctgg gccaaattcg
      541 acaaagccct cgcccgaatc gcgccactca catcagaaca ccttttcaac agcacctcca
      601 agcaccaaat cccccacacc tcactctctt cactctccac gcccaacgga tccttcaacg
      661 acatcaccag attcaaaatg agatcaaccg ccaaaagccg aagactggac ttcccctgag
      721 ccatctgcac aacatacttc acaaacgcaa tttgatcatc aaaacccatc actttaacaa
      781 cctccatgat ggactccaca gccaatgctc tcggctcggc cttctccggc gccttcttcg
      841 ccaaatacct cggaaaatta accagcgcct ttttcacatc atcacattga ttaccaagac
      901 tcgtgacaaa tccgagcgca aaagtcctcg cctgcgactt cgccatgaga acaagcgaac
      961 acagcgattt caaaacctcc gcagcagtgt tggaaggttc accgtgctcg gatttgagaa
     1021 cttccttcag cacgtgcgag cagaggctaa gcagtctgct atacaccgcc gcgttcccgc
     1081 acgtgttgag cgaggtcacg gggatttcag ccacggtttg gatcagcgac ttcagagtct
     1141 cggggaagcg gttgaggtgg attaaaccca tcacttcgac tagcttctcc agcacgcgga
     1201 ggagcaccct ggggtcgtgt tgggaactag tgtcgggcga atcgtcctcg ttttggggat
     1261 ttttcgcgcg accgcgaccc gggcgctttc gtttgccatt ctgcgagttg tcttgggccg
     1321 ggccagggtg ggagtgggcc ttgcaggagc ggcggaggga gcggaggaag gaaagaaacg
     1381 acattggtgt aaagagtgta aaaacgggtg cgttcggagc gaggagaagc gagaggaaga
     1441 catcggaagc gagaagagag tggtgcggag gggaagagtc cattgcggaa gcaatgggag
     1501 gaatgagaga agacggagag agattcttcg atgaaagctc atcgtagaga gactctgaaa
     1561 gagagtgttt gaggagaaat agaagattcg ttagggtttg ttcggagagt ggtggtggtt
     1621 gagggtttcc acggagatcc tctagctcgg agattacgcg agacaccgtt tcctccattt
     1681 ccgtcatgct gtgtcttctt cctccacaca ttccaccacg ctctcttttc catttcaaat
     1741 tcccgctttt tcaattacgg gcctattatt ctatgggccg aaatagaagc ccaataggag
     1801 aactggacaa ggggttcttt tggtaatttc agcgggtgga actagaaact ggaaaccctg
     1861 gactataaat aagagttgca cacaagtttt tcgtccctct tctctcgcgt cgctccgcac
     1921 cagtttcacc gcacgaatat cttgttgttt ggagcgatcc gagaaaaact ctgtaaccat
     1981 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     2041 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     2101 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     2161 cgtgaccacc ttcacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     2221 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2281 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2341 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2401 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2461 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2521 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2581 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2641 gctggagttc gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaagtaaag
     2701 cggccgcccg gctgcactga ggcctcagca gatcgttcaa acatttggca ataaagtttc
     2761 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     2821 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     2881 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     2941 taggataaat tatcgcgcgc ggtgtcatct atgttactag atccgatgat aagctgtcaa
     3001 acatgagaat tagatctcaa ttgaattcgt aatcatggtc atagctgttt cctgtgtgaa
     3061 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     3121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     3181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3841 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     3901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5161 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     5221 taacctataa aaataggcgt atcacgaggc cctttcgtc
//
