LOCUS       40924_1134        4956 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM1-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4956)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4956)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      411..1673
                     /label=GmScreamM1 promoter
                     /note="GmScreamM1 promoter"
                     /regulatory_class="promoter"
     CDS             1676..2392
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2428..2680
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2735..2751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2759..2775)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2783..2813)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2828..2849)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3137..3725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3899..4756)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4757..4861)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgcag ccgcacaata
      421 caagtcgcct tctcaaaccc ttcctcacga cattctcgga ccttatgaca ccgtcaccaa
      481 aacaatgatc cacgcgatat taggcgcgtg caaatcactc taatccgaaa ctagtagaca
      541 tgggaagcac gagctatacg cgagcgtttc aattgccgcc acgaaagcag agaaggccag
      601 aaacggaacc acggtaaaat ggtaagggta ttttcgtaaa cagaagaaaa gagttgtagc
      661 tataaataaa ccctctaacc cacggcgcac tattctcttc actccttcgt tcactcttct
      721 tctcttgcgg ctagggtttt agcgcagctt cttctaggtt cgttctcttc cgccgctcta
      781 tggattttaa accttcgaat catgtttatt ccattgaatt atgttgcttg cagtttatat
      841 tttctgaatc tgtagttgtt gtcttcaatt tatcctatgc tttatagatc aatcttttgt
      901 gtgtgtagta cgtaattttt gttctttttg cttttcgttc aagttgttgg gaataatcgg
      961 ggtatcatgt tttgatattg tttgttttct tttttgactg cttaataatt tttaagttgg
     1021 ttttggtttt ggggttttat gtgcttgtta tattcaaatc tttggatcca gatcttacaa
     1081 aagttttggg tttaaggatg tttttggctg atgatgaata gatctataaa ctgttccttt
     1141 taatcgattc aagcttagga ttttactagg cttttgcgaa taaatacgtg acagtaagct
     1201 aattatgtcc tttttttgtc tcaatcatat ctgtctgggt gtgccataat ttgtgatatg
     1261 tctatctggt agaatcttgt gttttatgct ttacgatttg gtatacctgt ttttgaactt
     1321 gttgtatgat gggtatttag atcaccctat cttttttatg cttctggaag ttttatgtaa
     1381 atgtcgaata tcttaatgtt gttgaactta taatgttgtg ttgatgtatg tatgatggtt
     1441 ttgacaactt ttttcactgg ttctgaaagt tttatgtaaa ttgcaaatat gttaatgttg
     1501 ttgaacttat tttttttcct tcgatgttgt tttgatgtat gtatgatggt tttcaccgta
     1561 gtttctatgg ctaatatctt aatgttgttg agcttatttt tttccttata tgttgtgttg
     1621 atgtatgtat gatggttttg acaacttttt tagtttcttt gcagatttaa ggaccatggt
     1681 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1741 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1801 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1861 gaccaccttc acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1921 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1981 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2041 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     2101 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     2161 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     2221 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     2281 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2341 ggagttcgtg accgccgccg ggatcactca cggcatggac gagctgtaca agtaaagcgg
     2401 ccgcccggct gcactgaggc ctcagcagat cgttcaaaca tttggcaata aagtttctta
     2461 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     2521 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     2581 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     2641 gataaattat cgcgcgcggt gtcatctatg ttactagatc cgatgataag ctgtcaaaca
     2701 tgagaattag atctcaattg aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     2761 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2821 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2881 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2941 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3001 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3061 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3121 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3181 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3241 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3301 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3361 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3421 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3481 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3541 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3601 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3661 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3721 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3781 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3841 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3901 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3961 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4021 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4081 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4141 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4201 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4261 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4321 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4381 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4441 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4501 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4561 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4621 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4681 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4741 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4801 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4861 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     4921 cctataaaaa taggcgtatc acgaggccct ttcgtc
//
