LOCUS       40924_1124        5206 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScream8-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5206)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5206)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1923
                     /label=GmScream8 promoter
                     /note="GmScream8 promoter"
                     /regulatory_class="promoter"
     CDS             1926..2642
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2678..2930
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2985..3001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3009..3025)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3033..3063)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3078..3099)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3387..3975)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4149..5006)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5007..5111)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttagacgc gacattgacc
      421 attgaataga gtgatggacg tacacttttt tgttttatag catagcattt ttaaatggtt
      481 gatgcccata cttttttcca ttgggattaa agtttgggag gtggcggtaa attctttcca
      541 tctgcacgtc gaaagcattt gggattgtgc tcatattgtt tttaacaagt ggggttgtcc
      601 tcatattata acttcttttt ttttaactat attttaagcg gaagaaaaaa atattgtggg
      661 gagagctacc ccaaaacttc taatgtagat ccgtttctaa atgtataata ttggtttatg
      721 tgacaatatt tttctttttt tgtgtgtggt aagcatggtg ttgtactata tcataattac
      781 acaaatatac tttaatatta attttttgtt atatttcaaa aaggataaac gtataatcta
      841 tctcaaatta tacaagtaat cattcaatta ttaaaaatca cactacatag tttcctaaga
      901 attttcaaat tagttcgtga ccattagtaa aatatattaa tgtgaaaatt aaatcaataa
      961 atatttaata ttttagagac tatttatata attttattac tttaaaggtt atttagagag
     1021 agagtaatac tctaaaatgg atgattttat agaagaagga tttggattat ttttacaaac
     1081 tcaaattaag tttacgcatt ttagtccaaa cgtgtacacc tctggtggga actgggaact
     1141 gggaactgag gaaatgaagg aaggttgaaa atgactttca caagccgcac tgtgccaagc
     1201 cccttgaaat agctgacttt ctcagctggt gttgctcgtt tgctttgcca agtgcaaaca
     1261 atttttgcca tttctcaata aaaaaacatg gaatctatcc gaaatcctga aatctttgtg
     1321 caagttccgt tacttactac tcttttttct cttaacatta tacttactat tacttattta
     1381 tattttttta atatgtcaaa taatatgaag tatttgtaca tttttatcct attatgttta
     1441 tgtttataaa taaaataaaa ttttattatt aaaaaatata ttaagaaatt aataaaatat
     1501 aattaattta aaatgaatta actggttgac gcatgaaatt agttgggcga cgtcagaaac
     1561 acttcatatt ttccgtggct tctacgcacc agagtccaaa atttaaagtt ttgtagctga
     1621 aaataggaaa agaaaagttg caacgaatcc cgtagcctag gcacatcaca tggtcagtaa
     1681 ttagtttaat aaatgctagc tcgtagtgag tgctggatca tgatgaaagc atcaacatca
     1741 ccctttaacc taaagtatta aaatgttggt ttattattat tactcatagc tcaaaaccca
     1801 attttaccct accaattaag gtctctcata cagtagtatt ttatttgcac tacgtactgg
     1861 ctactttatt tatttaagca gaagcaggtg agtgagtgag tgagcaagag tgaagagcga
     1921 agaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1981 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2041 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2101 ccaccctcgt gaccaccttc acctacggcg tgcagtgctt cagccgctac cccgaccaca
     2161 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2221 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2281 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2341 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     2401 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     2461 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2521 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     2581 tggtcctgct ggagttcgtg accgccgccg ggatcactca cggcatggac gagctgtaca
     2641 agtaaagcgg ccgcccggct gcactgaggc ctcagcagat cgttcaaaca tttggcaata
     2701 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     2761 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     2821 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     2881 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc cgatgataag
     2941 ctgtcaaaca tgagaattag atctcaattg aattcgtaat catggtcata gctgtttcct
     3001 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3061 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3121 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3181 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3241 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3301 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3361 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3421 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3481 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3541 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3601 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3661 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3721 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3781 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3841 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3901 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3961 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4021 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4081 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4141 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4201 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4261 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4321 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4381 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4441 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4501 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4561 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4621 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4681 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4741 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4801 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4861 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4921 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4981 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5041 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5101 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5161 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
