LOCUS       40924_1119        5048 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScream7-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5048)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5048)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1765
                     /label=GmScream7 promoter
                     /note="GmScream7 promoter"
                     /regulatory_class="promoter"
     CDS             1768..2484
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2520..2772
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2827..2843)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2851..2867)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2875..2905)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2920..2941)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3229..3817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3991..4848)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4849..4953)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgtctca tccacgagat
      421 agaaacaaaa tatataaaag ggctcattat gcttatttag ttcatcaaga agctaggaaa
      481 atgagtacgt agaatgaaca tttaataatg gacgtgagag aagttaatcg ctgacagcca
      541 tgtgccgacc atgtttttta taaatgaaaa gaaagaaatg ttcgtatata ataattaacg
      601 gacacaagaa ccttgttaat aattatcatt atcttttttt ttttgttttt attttccgaa
      661 aaacttgttt ctccaatcat tgatgtgtat ttctattctc tctccatttc caactcctga
      721 ctgagaagtg gatttcatat caacattagc aattagtaga atactatcat ctttcacgct
      781 acaaaacatt ggtactttgg taggtaaaga tttgcaaaca cgaataagta attaagaaag
      841 gttcatacac attcaatgat tctggattcc taccttacgt tatttgtttc gaaataccta
      901 gatgagagca tcttgttatt tattactaca tattaatttt ccctgtgtac cttgtcgtag
      961 tttaaattta ttattttttc aatcataaat aaatataaga aatatttttt tcttaatata
     1021 attttatttt atatttaaaa ataaatcata atttgaaaga gctacaaatt tataccacat
     1081 gtgggaagta ttgttggttt ctccaaccat acttattgag aataacttga atttatattc
     1141 aacgtattaa ttgcttcacc tttaacgtgc caaaataata ataataaaaa acttaaaact
     1201 actgtattaa tcgcgtgtgg ttgaatggag gcaaattcta ttctaaaaaa gaaaagcatt
     1261 aacaaaagga gaaaagaaaa actgttgaca cctgacagca gtaacaggga actgggaagt
     1321 agcagtagga gtatttgcgt gttggtttcc aactctggaa tccaccgtgc caaactgcga
     1381 atgcaggaga aatcgacacg tgtccatttg caggcgcgag ttgaacgtga caatgcacca
     1441 ccgcccagca tcgaacgcag ccaaggacca cgtcgaaacc acagtaatcc acgttccagt
     1501 gctgcgcgga acatggtcgg tctttctagg agtggttgga atcacgccag ctaggacaaa
     1561 ccccatcaat cattggtcat tatcaaacaa aacatttcaa aaattcaaca tattacgcct
     1621 cgggacccac ctcccactac acctcaccct cacttctatt aactcgaaca cattcgggtt
     1681 ataaatccgc aaccctcctt ctcactcact cactcactca ctcactcact cgcaagcaaa
     1741 aagaaagaat cccaggcgag gagaaccatg gtgagcaagg gcgaggagct gttcaccggg
     1801 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1861 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1921 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg cgtgcagtgc
     1981 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2041 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2101 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2161 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2221 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2281 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2341 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     2401 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2461 cacggcatgg acgagctgta caagtaaagc ggccgcccgg ctgcactgag gcctcagcag
     2521 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     2581 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     2641 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     2701 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     2761 tgttactaga tccgatgata agctgtcaaa catgagaatt agatctcaat tgaattcgta
     2821 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2881 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2941 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     3001 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     3061 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3121 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3181 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3241 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3301 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3361 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3421 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3481 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3541 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3601 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3661 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3721 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3781 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3841 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3901 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3961 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4021 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4081 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4141 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4201 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4261 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4321 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4381 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4441 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4501 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4561 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4621 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4681 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4741 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4801 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4861 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4921 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4981 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     5041 ctttcgtc
//
