LOCUS       40924_1114        4831 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScream6-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4831)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4831)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1548
                     /label=GmScream6 promoter
                     /note="GmScream6 promoter"
                     /regulatory_class="promoter"
     CDS             1551..2267
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2303..2555
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2610..2626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2634..2650)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2658..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2703..2724)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3012..3600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3774..4631)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4632..4736)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttttggtt tccgctcaga
      421 ataagaataa tttgataaat tcatttttag gcaattaaga atatttattt gactaacttt
      481 ttaattgaaa taaatttaca ataaatactc aatttatctt tcacaatcaa aagattgaga
      541 tgttgtaaga tctccgataa tatacttata tcttttcatt tattacgttt tcaaatttga
      601 attttaatgt gtgttgtaag tataaattta aaataaaaat aaaaacaatt attatatcaa
      661 aatggcaaaa acatttaata cgtattattt aagaaaaaaa tatgtaataa tatatttata
      721 ttttaatatc tattcttatg tattttttaa aaatctatta tatattgatc aactaaaata
      781 ttttttatat ctacacttat tttgcatttt tatcaatttt cttgcgtttt ttggcatatt
      841 taataatgac tattctttaa taatcaatca ttattcttac atggtacata ttgttggaac
      901 catatgaagt gtccattgca tttgactatg tggatagtgt tttgatccag gcctccattt
      961 gccgcttatt aattaatttg gtaacagtcc gtactaatca gttacttatc cttcctccat
     1021 cataattaat cttggtagtc tcgaatgcca caacactgac tagtctcttg gatcataaga
     1081 aaaagccaag gaacaaaaga agacaaaaca caatgagagt atcctttgca tagcaatgtc
     1141 taagttcata aaattcaaac aaaaacgcaa tcacacacag tggacatcac ttatccacta
     1201 gctgatcagg atcgccgcgt caagaaaaaa aaactggacc ccaaaagcca tgcacaacaa
     1261 cacgtactca caaaggtgtc aatcgagcag cccaaaacat tcaccaactc aacccatcat
     1321 gagcccacac atttgttgtt tctaacccaa cctcaaactc gtattctctt ccgccacctc
     1381 atttttgttt atttcaacac ccgtcaaact gcatgccacc ccgtggccaa atgtccatgc
     1441 atgttaacaa gacctatgac tataaatatc tgcaatctcg gcccaggttt tcatcatcaa
     1501 gaaccagttc aatatcctag tacaccgtat taaagaattt aagatatacc atggtgagca
     1561 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1621 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1681 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1741 ccttcaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1801 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1861 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1921 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1981 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2041 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2101 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2161 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2221 tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagtaa agcggccgcc
     2281 cggctgcact gaggcctcag cagatcgttc aaacatttgg caataaagtt tcttaagatt
     2341 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     2401 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     2461 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     2521 attatcgcgc gcggtgtcat ctatgttact agatccgatg ataagctgtc aaacatgaga
     2581 attagatctc aattgaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2641 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2701 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2761 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2821 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2881 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2941 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3001 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3061 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3121 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3181 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3241 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3301 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3361 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3421 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     3481 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3541 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3601 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3661 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3721 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3781 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3841 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3901 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3961 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4021 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4081 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4141 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4201 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4261 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4321 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4381 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4441 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4501 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4561 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4621 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4681 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4741 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4801 aaaaataggc gtatcacgag gccctttcgt c
//
