LOCUS       40924_1109        5376 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScream5-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5376)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      411..2093
                     /label=GmScream5 promoter
                     /note="GmScream5 promoter"
                     /regulatory_class="promoter"
     CDS             2096..2812
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2848..3100
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3155..3171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3179..3195)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3203..3233)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3248..3269)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3557..4145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4319..5176)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5177..5281)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgcag cggagtgaag
      421 tgggtactgt cttcgcgaat ctttcacccc gactttttaa catgtcttca taatctgata
      481 taataagatg gattaaaatt tattatttca tttatccccg accctgtcat gtattctatt
      541 taaattagaa aattattttt tttaaagaaa atattaaaaa tttgatttta aaaaaaataa
      601 tttgattgtt aaacatttat ttttaattat ttatatgtca ataaatttac tatagcgcat
      661 gtgcctacaa aaatcgttaa aaaaaatatt aaattatgta aaaaatttaa aataaaatta
      721 actagtaata aaaattctag atcttttgat taaattatgc aaaaatatta aatagtagga
      781 cagatctaga ttcgggattg gggcgaatat aataaattca tacccaattc atacctaact
      841 tttgattata aaaaaaccta aactcaaatc catattcgat taacttaaat attatccatc
      901 aaaatcggaa tcaaaccaac aagtactagt tttttttgtc atgtcaatta gtagttttcc
      961 ttttaatatt agtatgcatt attgtacctt ttatcgaaat gtattattat attgaacctt
     1021 ttatcatatc aaagatctag acaaaataag ttactccaac ttgtcggttg atgataacat
     1081 caatatgcat tattgcacta tgattgatta tgtttttgtt atttttttta gtaaataatt
     1141 aaattagtcc ttaagattgt catattaatc agttccaaaa ataaagtgaa actcaaatga
     1201 gtctcatatt ttttaagata ttagctacat ctcgattata aaaaaaaaca taatttatat
     1261 ttattaaaga aattagttaa ttttattaaa ttgagttatt tttaattatt ttttaaatta
     1321 ctttttattt aaacttgata ttaactataa aaaatctttg attttattaa atgaaaatta
     1381 ttttaaaaat aattttatta agtaagattt taataattaa aatttattta tattatagaa
     1441 aaaaataaga aaattttgtt gtttatattt gagattttga gggtgtaagt ctccaaaaag
     1501 ttcttatttt ttgttttgga ttcatgtgat attactatac ttaaattcgt ataaattacg
     1561 aagtaaaaat gaaggctata ttcgtaaata caaactatga gttggtctat aaataacgtt
     1621 caagtccctc ttcttcgtca cacagctatt cctcttaagc ttgctactga gtttccacac
     1681 tgcatacctt aggttcagtc tctgactagc ttttaatttt tctctttacg agtttattaa
     1741 ttattgttgt tgtgattcaa atatcataaa cctccactca attaatattt taattttcac
     1801 ttaccctaaa cacttcatct cactgtaaac cttatataat ttacccaatt aatattcaag
     1861 ttaattttca gttttaactt accttataca agcacttgat ctcattcttt agattctctt
     1921 tgcatctgac tagccctatt ttgtgccact ctagcttgtt tctaatattc ttggaggtgt
     1981 tgtattgtac aaattaattt tgcatgcagt atatgtatat atatttcata tttttttctg
     2041 attatattaa tttattttgg acaggtcttc cgaattcgaa gttaatttgc accccatggt
     2101 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2161 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2221 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2281 gaccaccttc acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     2341 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2401 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2461 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     2521 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     2581 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     2641 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     2701 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2761 ggagttcgtg accgccgccg ggatcactca cggcatggac gagctgtaca agtaaagcgg
     2821 ccgcccggct gcactgaggc ctcagcagat cgttcaaaca tttggcaata aagtttctta
     2881 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     2941 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     3001 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     3061 gataaattat cgcgcgcggt gtcatctatg ttactagatc cgatgataag ctgtcaaaca
     3121 tgagaattag atctcaattg aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     3181 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3241 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3301 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3361 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3421 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3961 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4081 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4141 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4201 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4261 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4321 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4381 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4441 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4501 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4561 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4621 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4681 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4741 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4801 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4861 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4921 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4981 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5041 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5101 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5161 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5221 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5281 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     5341 cctataaaaa taggcgtatc acgaggccct ttcgtc
//
