LOCUS       40924_1104        5121 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScream4-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5121)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5121)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1838
                     /label=GmScream4 promoter
                     /note="GmScream4 promoter"
                     /regulatory_class="promoter"
     CDS             1841..2557
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2593..2845
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2900..2916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2924..2940)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2948..2978)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2993..3014)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3302..3890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4064..4921)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4922..5026)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttatctgg gagagatgaa
      421 caatcgtttt atgtgtccct atatcacgtt ttccacttta gagagccgta caagattctg
      481 gaattagata tcattatatc aagagggaaa aaaactaaaa tgatattttg ctttttatat
      541 ataaaatatt ttaaaaaatt atcaatattt ttataaattt tgttattttg tttctcaagt
      601 gacactcaat tcattcatct cattatttaa tttttacaaa cttttagatt ttaatataaa
      661 ttcttcttag attttaaaat tttcattttg attcttatta tttaaataat attaaacttg
      721 tttttcaatt tatattaatc tcgtcatatt taattaaaaa attaacgtta ggaattaaaa
      781 taataataat aataataata atataataaa taaaaaaggt caaaataaga aatccatatt
      841 aatttataaa attaaaaaat catttaagaa aaatatatag gaataaaata tttggcgaag
      901 acaaataccc aaaatcatgc atgtagcaga gtgatgtttc aattaataaa tcgcgaagac
      961 acaaataaat aaataaataa tattttatat aaaaaaaatc atttaagaaa aaaaaagtct
     1021 ataggaataa ataattttgc gaagactaat acccaaaatc atgcattgca tgtttagcag
     1081 agtgatgttt caatgttaga aacaataaaa taattaaatc agattcttgt cttttcttcc
     1141 acctccgcaa agaaaaataa agttggtgtc agcatttctg tctagctaca gtctggacta
     1201 gctagcttgc agcggcaggg tttttttttt ttactttttt tttacgtgta cacattgtaa
     1261 tacaagattt atgactacaa ttaattcaaa cgcttatttc aagttttaac catatttaac
     1321 tattaacttt tatcggacct agcttcgaca cgaatcatca gttccaacaa cttatttaac
     1381 accaactaga gagataatat atggatgaga aaaaatataa atatgatatg ataaaaataa
     1441 tgaatattaa taaagtctat aaaaaatatg gggtgtacaa atatgataat gcattccaaa
     1501 tctgtgttcc agagaaaagg gtttcgtgtc tcattgttca ataaaaagtc aatacctagc
     1561 caataattca aagatagtac tactagtagt acataatatc gtgtttccaa gaaggtgagt
     1621 atcgaagtga aaagtaatta actaaactta ttactttccc agttgttgat tggagttaaa
     1681 tggttaagaa gcaactataa aaggaggtgc actagctctg ttaagagcat aaccaaaacc
     1741 aaatattctt gggggaaaaa ccaaatattc ttgggggaga atggggtcac taatggcctc
     1801 tgcttctatc actcttgctt ttgccataat ccttttcacc atggtgagca agggcgagga
     1861 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1921 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1981 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta
     2041 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2101 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2161 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2221 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2281 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2341 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2401 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2461 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2521 cgccgggatc actcacggca tggacgagct gtacaagtaa agcggccgcc cggctgcact
     2581 gaggcctcag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     2641 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     2701 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     2761 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     2821 gcggtgtcat ctatgttact agatccgatg ataagctgtc aaacatgaga attagatctc
     2881 aattgaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2941 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3001 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3061 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3121 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3181 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3241 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3301 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3361 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3421 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3481 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3541 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3601 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3661 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3721 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3781 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3841 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3901 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3961 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4021 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4081 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4141 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4201 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4261 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4321 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4381 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4441 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4501 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4561 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4621 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4681 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4741 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4801 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4861 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4921 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4981 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5041 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     5101 gtatcacgag gccctttcgt c
//
