LOCUS       40924_1099        4914 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScream3-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4914)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4914)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1631
                     /label=GmScream3 promoter
                     /note="GmScream3 promoter"
                     /regulatory_class="promoter"
     CDS             1634..2350
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2386..2638
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2693..2709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2717..2733)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2741..2771)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2786..2807)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3095..3683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3857..4714)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4715..4819)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgctaga tccaccgatc
      421 aatcgtatat atatacgcat aaaatcgacg cctctgtatt ttttagactg tagcccaaat
      481 tcactatccg aataaaataa gggaggcacg tgtacgtaat ttatatcata tgatagccat
      541 gcatatgcac acgtgcagaa gagctgttac cctctatacg tgtactcacc ttctcatcct
      601 ctctgaatat tttgagtgct cttcctagtt atctagtaat gcatgaaatt aaacttacta
      661 aatgtttctt caatttaaag aaataattgt ttatctgttt caattttttt aagagaattt
      721 taaaaagata attgtttcgg ggagagagat ataaaaaaga aaagggagaa atattaaaat
      781 gtactaaata atatgataag aaaagagaga aaaataaaag agaaaatttg tatatagtta
      841 taattattca tgtaataagg attcatctct caactgaaaa tatacttaat gcagaagaaa
      901 aaatcattat ttacaaacgt tgagtcttga gtgggaaaag aggaggcgcc gttactatac
      961 aatataagat catagtactg acaaaatgca cagtaaaaca gttcaaattg agaaggattc
     1021 ttaacacacc atagtattta atatatatct ttacagagac aattatgctg gaggattcag
     1081 gcaaagatta tatattgtgg atttgttttt taataattaa cgcatcatat gaaagatcga
     1141 tgatatatac taatggttat aagaaaaata tttaacagtt tctataacct ttttctttta
     1201 tcttttactg taatattatt tattttattt cacattttta atcagcttat ctcatttata
     1261 aacgaaattg tataaaaata tacatgatga actgaataga acaatattga tctgatattc
     1321 tcatattgta taagaggata gactttgaga cgcggagaat ctgtaggagg ggaccattca
     1381 gagtgcctcc aattttggtg ttgttcattg taccattgca aatataaacg aagcatgcat
     1441 gcttatgtat gaggtgtaac aaaattggaa acaatagcca tgcaaggtga agaatgtcac
     1501 aaactcagca acccttattc attgacgtgt ccctcagtca ctctcctctc atacctataa
     1561 atcaccactc ctcatgttct ttccaattca ccaactcctt caaacttaat tattaacact
     1621 tccttagttc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1681 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1741 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1801 gccctggccc accctcgtga ccaccttcac ctacggcgtg cagtgcttca gccgctaccc
     1861 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1921 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1981 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2041 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     2101 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     2161 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2221 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     2281 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactcacg gcatggacga
     2341 gctgtacaag taaagcggcc gcccggctgc actgaggcct cagcagatcg ttcaaacatt
     2401 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     2461 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     2521 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     2581 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatccg
     2641 atgataagct gtcaaacatg agaattagat ctcaattgaa ttcgtaatca tggtcatagc
     2701 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2761 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2821 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2881 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2941 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3001 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3061 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     4861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
