LOCUS       40924_1084        4921 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScream1-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4921)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4921)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1629
                     /label=GmScream1 promoter
                     /note="GmScream1 promoter"
                     /regulatory_class="promoter"
     CDS             1641..2357
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2393..2645
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2700..2716)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2724..2740)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2748..2778)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2793..2814)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3102..3690)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3864..4721)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4722..4826)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgctgag cataaacccg
      421 agagaagctc gaaccaaacc cacactaaag gaataaataa aaagacacaa tcgggcggtt
      481 tcaaattgtt tattcggact atacgggttt aaaaattgct tacatggggt tactttattc
      541 ggatgagggt ttaaaaaact tcctccggag aagaagaaag tgtttgaggc cgcaaaagaa
      601 accaagccac aacgcagatt ggaacacacc caagaatccg ttgcattcac taagcccgaa
      661 cctgacaaaa tcgagaggct aatgagttag agacaatcca gattattttc tatcagaaat
      721 acccacagtt tttaaactct gactttgtaa tttgttaatt gattgatatg tcattactaa
      781 cctacctatg ataaagagtc aaaataaatt ttttaagata taagaatcca atacaaagaa
      841 aaaaaaatta aaattagaac tcaaaataaa attaatcata tttattatag acctaaaaca
      901 tataagtatt tttgtttaac taatataatt gtttaaaact tagtcatttt cttcttattt
      961 tcgcttactt tttttttata ttgacaatca aaacgaggat aatccaaaaa acaaaatgtc
     1021 cctaatatat aatttctcta tccaagaaat tattatatat acagtacaaa ttattctcca
     1081 ctaatcttta tgatatgtaa attaaagtat gattaattat ttcctaatta aattaaaaaa
     1141 aattgagtaa ctagtatcac ataaagatct gtcaaaacca tgaatagtga taatttcata
     1201 acacctaata atttctctat ccctagagtg aggtcatttt tggacggccc ttcttctcac
     1261 tttgaggatc ccatcattgt cacacggtgt attcatataa tttcatatgt cataaacccg
     1321 cgaacatgaa atgaaaccat ggtccccctc tccaccaccg ttttctggca attgcatgca
     1381 atacaaacac acttggtatt tgtcacataa tgttgatgtc gaactgtcga agccacctca
     1441 cacccatgaa cttaatgagg tgtaacacac aaggcttcca tagccatgca tactgaagaa
     1501 tgtctcaagc tcagcacccc actcctgtga cgtgtccctc acccaccttc ctctcttccc
     1561 tataaataac cacgcctcag gttctccgct tcacaacaca aacattctct ccattgtctt
     1621 tgaacactcc tcgaggatcc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1681 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1741 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1801 tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc
     1861 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1921 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1981 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2041 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2101 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2161 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2221 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     2281 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca
     2341 tggacgagct gtacaagtaa agcggccgcc cggctgcact gaggcctcag cagatcgttc
     2401 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     2461 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     2521 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     2581 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     2641 agatccgatg ataagctgtc aaacatgaga attagatctc aattgaattc gtaatcatgg
     2701 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2761 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     2821 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2881 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2941 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3001 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3061 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3121 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3181 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3241 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3301 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3361 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3421 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3481 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3541 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3601 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3661 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3721 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3781 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3841 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3901 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3961 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4021 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4081 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4141 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4201 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4261 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4321 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4381 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4441 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4501 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4561 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4621 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4681 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4741 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4801 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     4861 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     4921 c
//
