LOCUS       62056_981        3796 bp DNA     circular SYN 03-DEC-2019
DEFINITION  Cloning vector GalR_NAR, complete sequence.
ACCESSION   MN207923
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Rondon RE, Groseclose TM, Short AE, Wilson CJ.
  TITLE     Transcriptional programming using engineered systems of 
            transcription factors and genetic architectures
  JOURNAL   Nat Commun 10 (1), 4784 (2019)
  PUBMED    31636266
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Rondon RE, Groseclose T, Short A, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2019) Chemical and Biomolecular Engineering, 
            Georgia Institute of Technology, 950 Atlantic dr. NW, 5110E, 
            Atlanta, GA 30332, United States
REFERENCE   3  (bases 1 to 3796)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "4784"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-2019) Chemical and Biomolecular Engineering, Georgia 
            Institute of Technology, 950 Atlantic dr. NW, 5110E, Atlanta, GA 
            30332, United States"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        596..673
                     /label=lacI promoter
     CDS             674..1711
                     /codon_start=1
                     /transl_table=11
                     /product="GalR NAR"
                     /label=GalR NAR
                     /protein_id="QFU95511.1"
                     /translation="MKPVTLYDVAEYAGVSNATVSRVVNQASHVSAKTREKVEAAMESL
                     SYHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDDR
                     YGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGEPD
                     ESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSR
                     YVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSLEASH
                     HATSD"
     protein_bind    1752..1773
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      1964..2508
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        3034..3136
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3137..3793
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
       61 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
      121 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
      181 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga
      241 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggttag
      301 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
      361 atccggaaca taatggtgca gggcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
      421 gccgggggac tgttgggcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
      481 gtcataagtg cggcgacgac accattcctt gcggcggcgg tgctcaacgg cctcaaccta
      541 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac
      601 catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
      661 cagggtggtg aatatgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
      721 taatgcgacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
      781 aaaagtggaa gcggcgatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
      841 ggcgcagcag accactgaaa cggtcggtct ggtcgttggt gatgtttccg atccgttttt
      901 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt ttttattgat
      961 tggcaacggt taccacaacg aacaaaaaga gcgtcaggcc attgagcaac tgatccgcca
     1021 tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgctgatt tagcctcatt
     1081 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
     1141 ttgtattgct ctggacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
     1201 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
     1261 tctgcaaggg tattacgatg cccttgctga aagtggtatt gcggccaatg accggctggt
     1321 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
     1381 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gtgcgatggg
     1441 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
     1501 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtgac
     1561 gatggcgacc caggctgccg aactggcttt ggcgctggcg gataatcgcc ctctcccgga
     1621 aatcactaat gtctttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
     1681 ggaggcaagt catcatgcaa ccagcgactg atgtacacga gaattggctt ggactcctgg
     1741 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc
     1801 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac taacatgaga
     1861 attacaactt atatcgtatg gggctgactt caggtgctac atttgaagag ataaattgca
     1921 ctgaaatcta gaaatatttt atctgattaa taagatgatc ttcttgagat cgttttggtc
     1981 tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg
     2041 ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg cagtcaccaa
     2101 aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac tcctctaaat
     2161 caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt ggactcaaga
     2221 cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc
     2281 agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg
     2341 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
     2401 gccgccaggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt
     2461 gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc
     2521 gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa tctccgcccc
     2581 gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
     2641 aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca gccttttttc
     2701 tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt aagccagtat
     2761 acactccgct agcgctgatg tccggcggtg cttttgccgt tacgcaccac cccgtcagta
     2821 gctgaacagg agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat
     2881 catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg aataaatacc
     2941 tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa
     3001 gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag gttccaactt
     3061 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
     3121 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     3181 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     3241 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     3301 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     3361 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     3421 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     3481 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     3541 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     3601 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     3661 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     3721 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     3781 gcagggcggg gcgtaa
//
