LOCUS       62056_1111        5877 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(622..846)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(925..1632)
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELY"
     intron          complement(1668..2493)
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     promoter        complement(2629..2832)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2833..3212)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             3838..4650
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      5195..5783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggggccttt agggggcgga gctatggaaa cgccagcaac gcggcctttt tacggttcct
       61 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
      121 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
      181 cagcgagtca gtgagcgagg aagcggaaga aattctatga tttctctcat tacttcctca
      241 catgttggag gcattttctc tccctctgca cttaatagcc tatcttgctt taatttcttc
      301 ccactcttgg aggctaggtt tggtttggtg ggctgatgag ggagggagag accgctccaa
      361 gtactttagc gggtgggatt gaaggcggag ccctcctgag ctatgagtgt cctatgagtg
      421 gggctggaac taagaaccag gggcgtggac agggtgtgtc acagagaagg ggatgtgcct
      481 gcttctttct ggcccaggag gaaccgggtc aattcttcag cacctgggta ccagagagag
      541 agagagagag agagagagag agagagagag gtaccagaga gagagagaga gagagagaga
      601 gagagagaga gagagaggta cccatagagc ccaccgcatc cccagcatgc ctgctattgt
      661 cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc
      721 tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac
      781 cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag
      841 gcacagcaga tccacaaaaa accaacacac agatctctag agctctgatc ttttattagc
      901 cagaagtgat ctggatcctc agttgtacag ttcatccatg ccatgtgtaa tcccagcagc
      961 tgttacaaac tcaagaagga ccatgtggtc tctcttttcg ttgggatctt tcgaaagggc
     1021 agattgtgtg gacaggtaat ggttgtctgg taaaaggaca gggccatcgc caattggagt
     1081 attttgttga taatggtctg ctagttgaac gcttccatct tcaatgttgt ggcgggtctt
     1141 gaagttcact ttgattccat tcttttgttt gtctgccatg atgtatacat tgtgtgagtt
     1201 atagttgtat tccaatttgt gtcccagaat gttgccatct tccttgaagt caataccttt
     1261 taactcgatt ctattaacaa gggtatcacc ttcaaacttg acttcagcac gtgtcttgta
     1321 gttgccgtca tctttgaaga agatggtcct ttcctgtaca taaccttcgg gcatggcact
     1381 cttgaaaaag tcatgccgtt tcatatgatc cgggtatctt gaaaagcatt gaacaccata
     1441 gcacagagta gtgactagtg ttggccatgg aacaggcagt ttgccagtag tgcagatgaa
     1501 cttcagggta agttttccgt atgttgcatc accttcaccc tctccactga cagagaactt
     1561 gtggccgtta acatcaccat ctaattcaac aagaattggg acaactccag tgaagagttc
     1621 ttctcctttg ctagccattt cttgcgcgcc cgtgtcgacg acggtgactg cagaaaagac
     1681 ccatggaaag gaacagtctg ttagtctgtc agctattatg tctggtggcg cgcgcggcag
     1741 caacgagtac tgctcagact acactgccct ccaccgttaa cagcaccgca acgggagtta
     1801 cctctgactc ttatcagaat acaacaactc agctgcctgc atcttcttct gccgctgcct
     1861 taagtcttcc atctgcgtca gccgtgcgag cccaatctcc acgctcattt tcagacacat
     1921 accctaccgc cacggccttg tgcggcacac tggtggtggt gggcattgtg ctgtgcctaa
     1981 gtctggcctc cactgttagg agcaaggagc tgccgagcga ccatgagccg ctggaggcat
     2041 gggaccaggg ctcggatgtg gaagctccgc cgctaccgga gaagagccca tgtccggaac
     2101 acgtacccga gattcgcgtg gagatcccac gctatgttta ataaaaactg cgggcacggg
     2161 ggacggcgtt gttgtatatg tgaatttgta aataataaat gggaccccat cctgtaaaaa
     2221 tacagagtcc gtgtcagtct ctgaaggaca gagtattggc atatagccaa tagagatagt
     2281 tgtggcaaag agccatgtta tggattagta atggaaagta tcgtcaccaa taggggagtg
     2341 gtcaataatg gtcaataacc cacacctata ggctaagcta taccatcacc tatagcataa
     2401 ggaagcgggg gtgtataggc cccaagccaa aaacagtata gcatgcataa gagccaaagg
     2461 ggtgtgccta tagagtctat aggcggtact tacgtcactc ttggcacggg gaatccgcgt
     2521 tccaatgcac cgttcccggc cgcggaggct ggatcggtcc cggtgtcttc tatggaggtc
     2581 aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat
     2641 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
     2701 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     2761 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
     2821 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     2881 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     2941 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     3001 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     3061 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     3121 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
     3181 ttactattaa taactagtca ataatcaatg tcaacatggc ggtaatgttg gacatgagcc
     3241 aatataaatg tacatattat gatatggata caacgtatgc aatggccaat agccaatctg
     3301 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     3361 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     3421 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3481 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3541 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3601 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3661 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatggggg
     3721 gggggggaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     3781 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     3841 agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct
     3901 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     3961 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     4021 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     4081 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     4141 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     4201 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     4261 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     4321 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     4381 atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     4441 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     4501 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     4561 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     4621 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     4681 tggcagagca ttacgctgac ttgacgggac ggcggctttg ttgaataaat cgaacttttg
     4741 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc
     4801 aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc
     4861 tcactttctg gctggatgat ggggcgattc aggcctggta tgagtcagca acaccttctt
     4921 cacgaggcag acctcagcgc cccccccccc cgagtcaggc aactatggat gaacgaaata
     4981 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5041 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5101 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5161 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5221 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5281 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5341 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5401 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5461 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5521 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5581 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5641 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5701 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5761 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5821 tttgctggcc ttttgctcac atgttcttcc tgcgtatcac ctgatgctgt ataaccc
//
