LOCUS       V015420                 7989 bp    DNA     circular SYN 20-JAN-2020
DEFINITION  Exported.
ACCESSION   V015420
VERSION     V015420
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2019) State Key Laboratory of Plant Genomics,
            Institute of Genetics and Developmental Biology, CAS, West Beichen
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7989)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (23-NOV-2019) State Key Laboratory of Plant Genomics, Institute of
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             918..941
                     /label="HRV 3C site"
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             966..3971
                     /gene="SMXL7"
                     /label="Protein SMAX1-LIKE 7"
                     /note="Protein SMAX1-LIKE 7 from Arabidopsis thaliana.
                     Accession#: O80875"
     promoter        4292..4396
                     /label="AmpR promoter"
     CDS             4397..5254
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5428..6016
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        6260..6337
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6338..7417
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    7433..7454
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7469..7499
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             7543..7716
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc ccggaattcc
      961 cggggatgcc gacaccagta accacggcga gacaatgttt aacggaagaa acagcgcgtg
     1021 cattagacga cgcagtctcc gtcgcgcgtc gtcgaagcca cgcacaaacg acgtcacttc
     1081 acgccgtttc aggtctttta acaatgcctt catcgattct ccgagaagtg tgtatctcac
     1141 gcgccgccca caacacaccg tactcttcac gtctccagtt tcgcgcgctt gagctctgcg
     1201 tcggcgtttc tctagacaga ttaccttctt cgaaatcaac accaacgacg acggtggaag
     1261 aagatcctcc ggtctcaaac tctctcatgg cggcgatcaa aagatctcaa gcgactcaac
     1321 gacgtcatcc agagacttat catcttcatc aaatccatgg aaacaacaac acggaaacga
     1381 cgtcggtttt gaaagttgag ttgaagtatt ttatactatc cattttagat gacccgattg
     1441 tgagtcgggt ctttggtgaa gccgggttta gaagcaccga cattaaactc gacgtgcttc
     1501 atcctccggt gacgtctcaa ttctcttcac ggttcacgtc aagatctcgt attcctcctc
     1561 tgtttttatg taacttaccg gaatctgact cgggtcgggt tagattcggg tttccgtttg
     1621 gtgatctcga cgagaactgt agaagaatcg gtgaagtttt agcgaggaaa gataagaaga
     1681 atccattact agttggtgtt tgtggtgttg aagctcttaa aacgtttact gattctataa
     1741 acagaggaaa gtttggtttt ttacctttgg agattagtgg gttaagtgta gttagtataa
     1801 agattagtga agttttagtt gatggatcaa gaatagatat aaagtttgat gatttaggga
     1861 gattgaagtc agggatggtt ttgaaccttg gagagttaaa ggttttagct agtgatgttt
     1921 tctctgttga tgtgattgag aagtttgtgt tgaagctcgc tgatttgttg aagcttcacc
     1981 gtgagaaact ttggtttatt ggaagtgttt cgagtaatga gacgtatttg aagctgattg
     2041 agaggtttcc aacgattgat aaagattgga atcttcatct tcttcctatt acatcttcaa
     2101 gtcaagggct ttatccaaaa tctagtttga tgggatcttt tgttccattt ggagggtttt
     2161 tctcatccac atcggatttt agaataccgt ctagtagctc gatgaatcag actttacctc
     2221 ggtgtcatct ttgtaacgag aagtatgagc aagaagtcac cgcctttgct aagtcggggt
     2281 cgatgatcga tgatcagtgt tcggagaagt taccttcttg gttacgaaat gtggagcatg
     2341 aacacgagaa aggaaatctc ggaaaggtta aagatgatcc aaacgtatta gcctctcgga
     2401 ttcccgctct gcagaagaaa tgggacgaca tttgtcaaag aatccatcaa actccggcgt
     2461 ttcctaaact cagttttcag cccgttagac cgcagttccc gcttcagctt ggttcttcta
     2521 gccagaccaa gatgagtctt ggaagcccaa ctgagaaaat tgtttgcact aggacgtctg
     2581 aaagctttca agggatggtg gctctaccgc agaatccgcc acaccaacct ggtttatcag
     2641 tcaaaatctc aaagccaaaa catactgagg atctctcaag ttcaacaacg aactcgcctt
     2701 tgagctttgt tacaacagat ttagggttgg gaacaatcta cgcatcgaag aaccaagaac
     2761 caagcacacc agtatcagtt gaaaggagag actttgaggt gatcaaagag aaacaattgt
     2821 tgtcagcttc gagatactgc aaagatttca agtctctcag ggaactactc tctcgaaaag
     2881 tcggttttca gaacgaagct gtgaatgcga ttagcgaaat tgtttgtgga tacagagatg
     2941 agtccaggag aagaaacaac cacgtagcaa ccacaagtaa tgtttggctt gctcttcttg
     3001 gacctgataa agccgggaag aagaaagtag cattagctct tgctgaagtc ttctgtggtg
     3061 gacaagacaa cttcatctgt gtggatttca agtcacaaga cagtcttgac gatagattca
     3121 gagggaaaac agttgttgat tacattgctg gcgaagtggc gagacgtgcg gattctgttg
     3181 ttttcatcga aaacgtggaa aaggccgagt tccctgatca gatcagattg tctgaggcta
     3241 tgagaactgg aaaactccgt gactcgcatg ggagagagat cagtatgaaa aatgttatag
     3301 ttgttgctac tatttctgga agtgataaag ccagtgattg tcatgttctt gaagaaccag
     3361 tcaaatactc cgaggaaaga gttctcaatg ccaaaaactg gacacttcag ataaaactag
     3421 cagacacttc aaatgttaac aagaatggtc ccaataagag aagacaggag gaggcagaaa
     3481 cagaggtgac agagcttcga gcccttaagt ctcagcgttc gtttcttgat ctaaatcttc
     3541 ctgtggatga gattgaagca aacgaagacg aagcgtatac aatgtcggag aacacagaag
     3601 cttggcttga ggattttgtg gaacaagtag atgggaaagt gacgttcaag cttattgact
     3661 ttgatgagtt agcaaaaaac ataaaaagga atattctttc gctatttcat ctgtcttttg
     3721 gacctgaaac acatctagag atcgaaaacg atgtgatcct taagattctt gctgctttaa
     3781 gatggtcatc agatgaagag aaaacatttg atcagtggct gcaaactgtt cttgctccaa
     3841 gctttgctaa agcgagacaa aagtgtgttc ccgctgctcc tttttctgtg aaacttgttg
     3901 cctctagaga gtctccggct gaagaggaga ctacagggat acaacagttt ccggcgagag
     3961 tcgaagtgat ctgatcgagc ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt
     4021 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     4081 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     4141 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtataatt cttgaagacg
     4201 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4261 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4321 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4381 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4441 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4501 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4561 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4621 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4681 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4741 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4801 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4861 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4921 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     4981 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5041 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5101 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5161 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5221 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5281 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5341 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6061 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6121 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6181 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     6241 ttcacaccgc ataaattccg acaccatcga atggtgcaaa acctttcgcg gtatggcatg
     6301 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga
     6361 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
     6421 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     6481 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     6541 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     6601 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     6661 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     6721 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     6781 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     6841 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     6901 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     6961 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     7021 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     7081 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     7141 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgtcaaccac
     7201 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     7261 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     7321 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7381 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7441 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7501 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacggat
     7561 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     7621 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     7681 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     7741 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     7801 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtaacctat
     7861 cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc
     7921 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
     7981 gttggaatt
//
