LOCUS       V016142                 7920 bp    DNA     circular SYN 20-JAN-2020
DEFINITION  Exported.
ACCESSION   V016142
VERSION     V016142
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2019) State Key Laboratory of Plant Genomics,
            Institute of Genetics and Developmental Biology, CAS, West Beichen
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (23-NOV-2019) State Key Laboratory of Plant Genomics, Institute of
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             918..941
                     /label="HRV 3C site"
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             966..3902
                     /gene="SMXL6"
                     /label="Protein SMAX1-LIKE 6"
                     /note="Protein SMAX1-LIKE 6 from Arabidopsis thaliana.
                     Accession#: Q9LML2"
     promoter        4223..4327
                     /label="AmpR promoter"
     CDS             4328..5185
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5359..5947
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        6191..6268
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6269..7348
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    7364..7385
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7400..7430
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             7474..7647
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc ccggaattcc
      961 cggggatgcc gacgccggtg actacggcga gagaatgctt gacggaagaa gctgctcgtg
     1021 ccctcgacga tgctgttgtt gtagctcgtc ggagaagcca cgcgcagacg acgtctcttc
     1081 atgcagtttc tgctctttta gctatgccgt cgtcgattct ccgtgaagtt tgcgtctcac
     1141 gcgccgctag gagtgttcct tactcgtcgc gacttcaatt ccgagctctt gagctctgcg
     1201 tcggtgtatc tctcgacagg cttccgtcgt cgaagtctcc ggcgacggaa gaagatccac
     1261 cggtttcgaa ttcgctcatg gcggcgatca aacggtctca ggcgaaccag agacggcatc
     1321 cggagtcgta tcatcttcag cagatccacg ctagtaacaa cggcggggga ggatgccaga
     1381 cgacggtttt gaaagtcgaa ttgaagtatt tcatactctc gatccttgac gatccgattg
     1441 tgaatcgggt attcggagaa gctgggtttc ggagctccga aattaagctc gatgtgcttc
     1501 accctccggt aacacaactt tcttcccgtt tctctagagg tcgttgtccg cctctcttcc
     1561 tctgtaatct tcccaactca gatccgaatc gtgagttccc gtttagtggg agcagtggtt
     1621 tcgatgaaaa ttcccggagg attggagaag tattaggcag gaaagataag aagaaccctc
     1681 tgcttattgg taactgtgct aatgaagctc ttaaaacgtt cacggattcg atcaacagtg
     1741 ggaagttagg gtttcttcag atggatatta gcggattaag cttgattagt atcgagaagg
     1801 agattagtga gattttagcc gatggatcga aaaacgaaga agagattcga atgaaagtgg
     1861 atgacctagg aagaactgta gagcaaagtg gctcgaaatc ggggatagtg cttaatctgg
     1921 gagagctcaa ggttttgacc agtgaagcca atgcggctct tgagattttg gtgtcgaagc
     1981 tttcggattt gctgaaacat gaaagtaaac aactttcgtt catcggatgt gtatcgagta
     2041 atgagactta cacgaagctt attgatcggt ttcctactat agagaaggat tgggaccttc
     2101 atgttcttcc aatcacagcc tctactaaac cttcgactca aggggtttat cccaaatcga
     2161 gcttgatggg atcctttgtt ccctttggag ggttcttctc atcaacatca aatttcagag
     2221 ttccattgag tagcacagtg aatcagacgc tctctagatg ccacctctgc aacgagaagt
     2281 atttgcaaga agtagccgct gttctcaagg ccggttcgag tctttctctg gctgacaaat
     2341 gttccgagaa gttagcccca tggctacggg ctattgagac caaagaagac aagggaataa
     2401 caggcagcag taaggcttta gatgacgcta atacatcagc ctcgcaaacc gctgctctac
     2461 agaagaaatg ggacaacata tgccaaagta tccatcacac tccggcgttt cctaaacttg
     2521 gttttcagtc ggtgagtccg cagttcccag ttcagactga gaagagtgtg agaactccta
     2581 caagctattt ggagacgcct aaactgctga atccgccaat ctcaaagcca aaacctatgg
     2641 aggatcttac ggcatcggtg actaaccgca cagtgagttt gcctttgagc tgtgttacta
     2701 cagattttgg gttgggagta atctatgcat ccaaaaacca ggaatcaaaa acaacgaggg
     2761 agaaaccgat gctggtgact ctaaactctt ctttagaaca tacatatcag aaagatttca
     2821 agtctctcag agaaatactc tctcgtaaag ttgcctggca gaccgaagct gtaaatgcca
     2881 taagccaaat tatctgcgga tgcaaaaccg actccacgcg aagaaaccaa gcaagcggaa
     2941 tttggctggc tcttcttgga cccgataaag tggggaagaa gaaagtggcg atgactcttt
     3001 ctgaagtctt ctttggtggt aaagtcaatt acatatgtgt agattttggg gcagagcatt
     3061 gttcccttga tgacaaattc agaggcaaaa cagtggtgga ttacgtaacc ggtgagttat
     3121 ctaggaaacc acactctgtt gttttactcg aaaacgtgga aaaagctgag ttcccggatc
     3181 agatgagatt gtctgaagct gtgagtacgg ggaaaatccg tgatttgcat ggaagagtga
     3241 ttagtatgaa aaatgtgatt gttgttgtga cgtctgggat tgccaaggat aatgccactg
     3301 accatgttat taaacctgtg aagtttcctg aggagcaagt tctcagcgcg agaagctgga
     3361 aactgcagat aaagctagga gatgctacta aatttggggt aaataagaga aaatatgagc
     3421 tagaaacagc gcaacgtgca gtgaaggtgc aacgttcata tctggatctg aatcttccag
     3481 tgaatgaaac agaatttagc cctgatcatg aggcagagga cagggacgct tggttcgatg
     3541 aattcattga aaaagtagat ggaaaagtga cgttcaaacc ggttgatttc gatgagttag
     3601 ccaagaacat tcaagagaag attggttcac attttgagcg gtgctttgga tccgaaacac
     3661 atctagaact tgataaagaa gtgatccttc agattctggc ggcttcatgg tcatcattat
     3721 catcgggcga agaagaaggg agaacaatag ttgatcagtg gatgcaaaca gttcttgctc
     3781 gaagctttgc tgaagcaaaa cagaagtacg gttcgaatcc catgttgggc gtgaagctgg
     3841 ttgcttcttc tagcggctta gcttccggag tagaattgcc ggcgaaggtg gatgtgatat
     3901 ggtgatcgag cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga
     3961 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     4021 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     4081 agccatgacc cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct
     4141 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4201 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4261 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4321 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4381 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4441 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4501 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4561 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4621 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4681 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4741 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4801 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4861 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4921 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4981 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5041 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5101 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5161 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5221 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5281 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5341 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5401 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5461 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     5521 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5581 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5641 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5701 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5761 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5821 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5881 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5941 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6001 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6061 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6121 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     6181 cataaattcc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc
     6241 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
     6301 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
     6361 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
     6421 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
     6481 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
     6541 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
     6601 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
     6661 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
     6721 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
     6781 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
     6841 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
     6901 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
     6961 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
     7021 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
     7081 gggatacgac gataccgaag acagctcatg ttatatcccg ccgtcaacca ccatcaaaca
     7141 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     7201 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     7261 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7321 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7381 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7441 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc
     7501 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7561 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7621 caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
     7681 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca
     7741 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg
     7801 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
     7861 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttggaatt
//
