LOCUS       40924_1059       11569 bp DNA     circular SYN 13-MAY-2021
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 11569)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11569)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15-
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(944..1801)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1802..1906)
                     /label=AmpR promoter
     primer_bind     complement(1981..2000)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        2172..2551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2552..2755
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     2752..2776
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2880..3596
                     /codon_start=1
                     /label=EmGFP
                     /note="Emerald GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    3612..3636
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5197..5211)
                     /codon_start=1
                     /label=SNAC tag
                     /note="tag for protein cleavage using Ni2+ ion (Dang et
                     al., 2019)"
                     /translation="GSHHW"
     protein_bind    complement(9331..9355)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9363..9404
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(9444..9463)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9488..9536
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9738..10166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9825..9844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10035..10056
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10180..10509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        10557..10604
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             10623..11018
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    11179..11312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11349..11365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11349..11365)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11362..11384)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11373..11389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11397..11427)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11442..11463)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     2761 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     2821 ccgggaccga tccagcctcc ggactctaga ggatccctac cggtgatatc ctcgagacca
     2881 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2941 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3001 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3061 tcgtgaccac cttcacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
     3121 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3181 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3241 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3301 agctggagta caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg
     3361 gcatcaaggt gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg
     3421 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3481 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3541 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggct
     3601 cgagcccatc aacaagtttg tacaaaaaag caggcttcga aggagataga accaattctc
     3661 taaggaaata cttaaccatg gtcgacatgg gacccggctg cgatttgctg ctgcggacag
     3721 cagctaccat cactgctgcc gccatcatgt cagacacgga cagcgacgaa gattccgctg
     3781 gaggcggccc attttcttta gcgggtttcc ttttcggcaa catcaatgga gccgggcagc
     3841 tggaggggga aagcgtcttg gatgatgaat gtaagaagca cttggcaggc ttgggggctt
     3901 tggggctggg cagcctgatc actgaactca cggcaaatga agaattgacc gggactgacg
     3961 gtgccttggt aaatgatgaa gggtgggtta ggagtacaga agatgctgtg gactattcag
     4021 acatcaatga ggtggcagaa gatgaaagcc gaagatacca gcagacgatg gggagcttgc
     4081 agcccctttg ccactcagat tatgatgaag atgactatga tgctgattgt gaagacattg
     4141 attgcaagtt gatgcctcct ccacctccac ccccgggacc aatgaagaag gataaggacc
     4201 aggattctat tactggtgag aaagtggact tcagtagttc ctctgactca gaatctgaga
     4261 tgggacctca ggaagcaaca caggcagaat ctgaagatgg aaagctgacc cttccattgg
     4321 ctgggattat gcagcatgat gccaccaagc tgttgccaag tgtcacagaa ctttttccag
     4381 aatttcgacc tggaaaggtg ttacgttttc tacgtctttt tggaccaggg aagaatgtcc
     4441 catctgtttg gcggagtgct cggagaaaga ggaagaagaa gcaccgtgag ctgatacagg
     4501 aagagcagat ccaggaggtg gagtgctcag tagaatcaga agtcagccag aagtctttgt
     4561 ggaactacga ctacgctcca ccaccacctc cagagcagtg tctctctgat gatgaaatca
     4621 cgatgatggc tcctgtggag tccaaatttt cccaatcaac tggagatata gataaagtga
     4681 cagataccaa accaagagtg gctgagtggc gttatgggcc tgcccgactg tggtatgata
     4741 tgctgggtgt ccctgaagat ggcagtgggt ttgactatgg cttcaaactg agaaagacag
     4801 aacatgaacc tgtgataaaa tctagaatga tagaggaatt taggaaactt gaggaaaaca
     4861 atggcactga tcttctggct gatgaaaact tcctgatggt gacacagctg cattgggagg
     4921 atgatatcat ctgggatggg gaggatgtca aacacaaagg gacaaaacct cagcgtgcaa
     4981 gcctggcagg ctggcttcct tctagcatga ctaggaatgc gatggcttac aatgttcagc
     5041 aaggttttgc agccactctt gatgatgaca aaccttggta ctccattttt cccattgaca
     5101 atgaggatct ggtatatgga cgctgggagg acaatatcat ttgggatgct caggccatgc
     5161 cccggctgtt ggaacctcct gttttgacac ttgatcccaa tgatgagaac ctcattttgg
     5221 aaattcctga tgagaaggaa gaggccacct ctaactcccc ctccaaggag agtaagaagg
     5281 aatcatctct gaagaagagt cgaattctct tagggaaaac aggagtcatc aaggaggaac
     5341 cacagcagaa catgtctcag ccagaagtga aagatccatg gaatctctcc aatgatgagt
     5401 attattatcc caagcaacag ggtcttcgag gcacctttgg agggaatatt atccagcatt
     5461 caattcctgc tgtggaatta cggcagccct tctttcccac ccacatgggg cccatcaaac
     5521 tccggcagtt ccatcgccca cctctgaaaa agtactcatt tggtgcactt tctcagccag
     5581 gtccccactc agtccaacct ttgctaaagc acatcaaaaa aaaggccaag atgagagaac
     5641 aagagaggca agcttcaggt ggtggagaga tgttttttat gcgcacacct caggacctca
     5701 caggcaaaga tggtgatctt attcttgcag aatatagtga ggaaaatgga cccttaatga
     5761 tgcaggttgg catggcaacc aagataaaga actattataa acggaaacct ggaaaagatc
     5821 ctggagcacc agattgtaaa tatggggaaa ctgtttactg ccatacatct cctttcctgg
     5881 gttctctcca tcctggccaa ttgctgcaag catttgagaa caaccttttt cgtgctccaa
     5941 tttatcttca taagatgcca gaaactgatt tcttgatcat tcggacaaga cagggttact
     6001 atattcggga attagtggat atttttgtgg ttggccagca gtgtcccttg tttgaagttc
     6061 ctgggcctaa ctccaaaagg gccaatacgc atattcgaga ctttctacag gtttttattt
     6121 accgcctttt ctggaaaagt aaagatcggc cacggaggat acgaatggaa gatataaaaa
     6181 aagcctttcc ttcccattca gaaagcagca tccggaagag gctaaagctc tgcgctgact
     6241 tcaaacgcac agggatggac tcaaactggt gggtgcttaa gtctgatttt cgtttaccaa
     6301 cggaagaaga gatcagagct atggtgtcac cagagcagtg ctgtgcttat tatagcatga
     6361 tagctgcaga gcaacgactg aaggatgctg gctatggtga gaaatccttt tttgctccag
     6421 aagaagaaaa tgaggaagat ttccagatga agattgatga tgaagttcgc actgcccctt
     6481 ggaacaccac aagggccttc attgctgcca tgaagggcaa gtgtctgcta gaggtgactg
     6541 gggtggcaga tcccacgggg tgtggtgaag gattctccta tgtgaagatt ccaaacaaac
     6601 caacacagca gaaggatgat aaagaaccgc agccagtgaa gaagacagtg acaggaacag
     6661 atgcagacct tcgtcgcctt tccctgaaaa atgccaagca acttctacgt aaatttggtg
     6721 tgcctgagga agagattaaa aagttgtccc gctgggaagt gattgatgtg gtgcgcacaa
     6781 tgtcaacaga acaggctcgt tctggagagg ggcccatgag taaatttgcc cgtggatcaa
     6841 ggttttctgt ggctgagcat caagagcgtt acaaagagga atgtcagcgc atctttgacc
     6901 tacagaacaa ggttctgtca tcaactgaag tcttatcaac tgacacagac agcagctcag
     6961 ctgaagatag tgactttgaa gaaatgggaa agaacattga gaacatgttg cagaacaaga
     7021 aaaccagctc tcagctttca cgtgaacggg aggaacagga gcggaaggaa ctacagcgaa
     7081 tgctactggc agcaggctca gcagcatccg gaaacaatca cagagatgat gacacagctt
     7141 ccgtgactag ccttaactct tctgccactg gacgctgtct caagatttat cgcacgtttc
     7201 gagatgaaga ggggaaagag tatgttcgct gtgagacagt ccgaaaacca gctgtcattg
     7261 atgcctatgt gcgcatacgg actacaaaag atgaggaatt cattcgaaaa tttgcccttt
     7321 ttgatgaaca acatcgggaa gagatgcgaa aagaacggcg gaggattcaa gagcaactga
     7381 ggcggcttaa gaggaaccag gaaaaggaga agcttaaggg tcctcctgag aagaagccca
     7441 agaaaatgaa ggagcgtcct gacctaaaac tgaaatgtgg ggcatgtggt gccattggac
     7501 acatgaggac taacaaattc tgccccctct attatcaaac aaatgcgcca ccttccaacc
     7561 ctgttgccat gacagaagaa caggaggagg agttggaaaa gacagtcatt cataatgata
     7621 atgaagaact tatcaaggtt gaagggacca aaattgtctt ggggaaacag ctaattgaga
     7681 gtgcggatga ggttcgcaga aaatctctgg ttctcaagtt tcctaaacag cagcttcctc
     7741 caaagaagaa acggcgagtt ggaaccactg ttcactgtga ctatttgaat agacctcata
     7801 agtccatcca ccggcgccgc acagacccta tggtgacgct gtcgtccatc ttggagtcta
     7861 tcatcaatga catgagagat cttccaaata catacccttt ccacactcca gtcaatgcaa
     7921 aggttgtaaa ggactactac aaaatcatca ctcggccaat ggacctacaa acactccgcg
     7981 aaaacgtgcg taaacgcctc tacccatctc gggaagagtt cagagagcat ctggagctaa
     8041 ttgtgaaaaa tagtgcaacc tacaatgggc caaaacactc attgactcag atctctcaat
     8101 ccatgctgga tctctgtgat gaaaaactca aagagaaaga agacaaatta gctcgcttag
     8161 agaaagctat caaccccttg ctggatgatg atgaccaagt ggcgttttct ttcattctgg
     8221 acaacattgt cacccagaaa atgatggcag ttccagattc ttggccattt catcacccag
     8281 ttaataagaa atttgttcca gattattaca aagtgattgt caatccaatg gatttagaga
     8341 ccatacgtaa gaacatctcc aagcacaagt atcagagtcg ggagagcttt ctggatgatg
     8401 taaaccttat tctggccaac agtgttaagt ataatggacc tgagagtcag tatactaaga
     8461 ctgcccagga gattgtgaac gtctgttacc agacattgac tgagtatgat gaacatttga
     8521 ctcaacttga gaaggatatt tgtactgcta aagaagcagc tttggaggaa gcagaattag
     8581 aaagcctgga cccaatgacc ccagggccct acacgcctca gcctcctgat ttgtatgata
     8641 ccaacacatc cctcagtatg tctcgagatg cctctgtatt tcaagatgag agcaatatgt
     8701 ctgtcttgga tattcccagt gccactccag aaaagcaggt aacacaggaa ggtgaagatg
     8761 gagatggtga tcttgcagat gaagaggaag gaactgtaca acagcctcaa gccagtgtcc
     8821 tgtatgagga tttgcttatg tctgaaggag aagatgatga ggaagatgct gggagtgatg
     8881 aagaaggaga caatcctttc tctgctatcc agctgagtga aagtggaagt gactctgatg
     8941 tgggatctgg tggaataaga cccaaacaac cccgcatgct tcaggagaac acaaggatgg
     9001 acatggaaaa tgaagaaagc atgatgtcct atgagggaga cggtggggag gcttcccatg
     9061 gtttggagga tagcaacatc agttatggga gctatgagga gcctgatccc aagtcgaaca
     9121 cccaagacac aagcttcagc agcatcggtg ggtatgaggt atcagaggag gaagaagatg
     9181 aggaggagga agagcagcgc tctgggccga gcgtactaag ccaggtccac ctgtcagagg
     9241 acgaggagga cagtgaggat ttccactcca ttgctgggga cagtgacttg gactctgatg
     9301 aattatcgcg gccgcactcg agatatctag acccagcttt cttgtacaaa gtggttgata
     9361 acgggaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat
     9421 gagtttaaac gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     9481 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
     9541 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     9601 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     9661 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcagatctgc gcagctgggg
     9721 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     9781 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     9841 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     9901 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     9961 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    10021 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
    10081 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
    10141 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
    10201 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    10261 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    10321 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
    10381 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    10441 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    10501 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
    10561 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
    10621 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
    10681 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
    10741 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
    10801 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
    10861 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
    10921 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
    10981 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacttcgt ggccgaggag
    11041 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
    11101 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
    11161 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    11221 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    11281 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
    11341 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    11401 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    11461 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    11521 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgct
//
