LOCUS       40924_1034       12226 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GAPTrapT2AIresMygro, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG.
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives
  JOURNAL   Stem Cell Reports (2016) In press
  PUBMED    27594589
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   Stanley E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2016) Cell Biology, Murdoch Childrens Research 
            Institute, Royal Children's Hospital, Flemington Road, Melbourne, 
            Victoria 3052, Australia
REFERENCE   3  (bases 1 to 12226)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12226)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-AUG-2016) Cell Biology, Murdoch Childrens Research Institute, 
            Royal Children's Hospital, Flemington Road, Melbourne, Victoria 
            3052, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    2110..2126
                     /label=lac operator
                     /note="lac operator"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="M13 rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     gene            2216..5559
                     /gene="GAPDH"
                     /label=GAPDH
                     /note="glyceraldehyde-3-phosphate dehydrogenase"
     misc_feature    2219..5556
                     /gene="GAPDH"
                     /label=left arm
                     /note="left arm"
     CDS             5584..5637
                     /codon_start=1
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5660..6246
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6256..7278
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    7310..11545
                     /label=right arm
                     /note="right arm"
     promoter        complement(11583..11601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11611..11627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(11768..12223)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatgnn
     2221 ngggaaggtg aaggtcggag tcaacgggtg agttcgcggg tggctggggg gccctgggct
     2281 gcgaccgccc ccgaaccgcg tctacgagcc ttgcgggctc cgggtctttg cagtcgtatg
     2341 ggggcagggt agctgttccc cgcaaggaga gctcaaggtc agcgctcgga cctggcggag
     2401 ccccgcaccc aggctgtggc gccctgtgca gctccgccct tgcggcgcca tctgcccgga
     2461 gcctccttcc cctagtcccc agaaacagga ggtccctact cccgcccgag atcccgaccc
     2521 ggacccctag gtgggggacg ctttctttcc tttcgcgctc tgcggggtca cgtgtcgcag
     2581 aggagcccct cccccacggc ctccggcacc gcaggccccg ggatgctagt gcgcagcggg
     2641 tgcatccctg tccggatgct gcgcctgcgg tagagcggcc gccatgttgc aaccgggaag
     2701 gaaatgaatg ggcagccgtt aggaaagcct gccggtgact aaccctgcgc tcctgcctcg
     2761 atgggtggag tcgcgtgtgg cggggaagtc aggtggagcg aggctagctg gcccgatttc
     2821 tcctccgggt gatgcttttc ctagattatt ctctggtaaa tcaaagaagt gggtttatgg
     2881 aggtcctctt gtgtcccctc cccgcagagg tgtggtggct gtggcatggt gccaagccgg
     2941 gagaagctga gtcatgggta gttggaaaag gacatttcca ccgcaaaatg gcccctctgg
     3001 tggtggcccc ttcctgcagc gccggctcac ctcacggccc cgcccttccc ctgccagcct
     3061 agcgttgacc cgaccccaaa ggccaggctg taaatgtcac cgggaggatt gggtgtctgg
     3121 gcgcctcggg gaacctgccc ttctccccat tccgtcttcc ggaaaccaga tctcccaccg
     3181 caccctggtc tgaggttaaa tatagctgct gacctttctg tagctggggg cctgggctgg
     3241 ggctctctcc catcccttct ccccacacac atgcacttac ctgtgctccc actcctgatt
     3301 tctggaaaag agctaggaag gacaggcaac ttggcaaatc aaagccctgg gactaggggg
     3361 ttaaaataca gcttcccctc ttcccacccg ccccagtctc tgtccctttt gtaggaggga
     3421 cttagagaag gggtgggctt gccctgtcca gttaatttct gacctttact cctgcccttt
     3481 gagtttgatg atgctgagtg tacaagcgtt ttctccctaa agggtgcagc tgagctaggc
     3541 agcagcaagc attcctgggg tggcatagtg gggtggtgaa taccatgtac aaagcttgtg
     3601 cccagactgt gggtggcagt gccccacatg gccgcttctc ctggaagggc ttcgtatgac
     3661 tgggggtgtt gggcagccct ggagccttca gttgcagcca tgccttaagc caggccagcc
     3721 tggcagggaa gctcaaggga gataaaattc aacctcttgg gccctcctgg gggtaaggag
     3781 atgctgcatt cgccctctta atggggaggt ggcctagggc tgctcacata ttctggagga
     3841 gcctcccctc ctcatgcctt cttgcctctt gtctcttaga tttggtcgta ttgggcgcct
     3901 ggtcaccagg gctgctttta actctggtaa agtggatatt gttgccatca atgacccctt
     3961 cattgacctc aactacatgg tgagtgctac atggtgagcc ccaaagctgg tgtgggagga
     4021 gccacctggc tgatgggcag ccccttcata ccctcacgta ttcccccagg tttacatgtt
     4081 ccaatatgat tccacccatg gcaaattcca tggcaccgtc aaggctgaga acgggaagct
     4141 tgtcatcaat ggaaatccca tcaccatctt ccaggagtga gtggaagaca gaatggaaga
     4201 aatgtgcttt ggggaggcaa ctaggatggt gtggctccct tgggtatatg gtaaccttgt
     4261 gtccctcaat atggtcctgt ccccatctcc cccccacccc cataggcgag atccctccaa
     4321 aatcaagtgg ggcgatgctg gcgctgagta cgtcgtggag tccactggcg tcttcaccac
     4381 catggagaag gctggggtga gtgcaggagg gcccgcggga ggggaagctg actcagccct
     4441 gcaaaggcag gacccgggtt cataactgtc tgcttctctg ctgtaggctc atttgcaggg
     4501 gggagccaaa agggtcatca tctctgcccc ctctgctgat gcccccatgt tcgtcatggg
     4561 tgtgaaccat gagaagtatg acaacagcct caagatcatc aggtgaggaa ggcagggccc
     4621 gtggagaagc ggccagcctg gcaccctatg gacacgctcc cctgacttgc gccccgctcc
     4681 ctctttcttt gcagcaatgc ctcctgcacc accaactgct tagcacccct ggccaaggtc
     4741 atccatgaca actttggtat cgtggaagga ctcatggtat gagagctggg gaatgggact
     4801 gaggctccca cctttctcat ccaagactgg ctcctccctg ccggggctgc gtgcaaccct
     4861 ggggttgggg gttctgggga ctggctttcc cataatttcc tttcaaggtg gggagggagg
     4921 tagaggggtg atgtggggag tacgctgcag ggcctcactc cttttgcaga ccacagtcca
     4981 tgccatcact gccacccaga agactgtgga tggcccctcc gggaaactgt ggcgtgatgg
     5041 ccgcggggct ctccagaaca tcatccctgc ctctactggc gctgccaagg ctgtgggcaa
     5101 ggtcatccct gagctgaacg ggaagctcac tggcatggcc ttccgtgtcc ccactgccaa
     5161 cgtgtcagtg gtggacctga cctgccgtct agaaaaacct gccaaatatg atgacatcaa
     5221 gaaggtggtg aagcaggcgt cggagggccc cctcaagggc atcctgggct acactgagca
     5281 ccaggtggtc tcctctgact tcaacagcga cacccactcc tccacctttg acgctggggc
     5341 tggcattgcc ctcaacgacc actttgtcaa gctcatttcc tggtatgtgg ctggggccag
     5401 agactggctc ttaaaaagtg cagggtctgg cgccctctgg tggctggctc agaaaaaggg
     5461 ccctgacaac tcttttcatc ttctaggtat gacaacgaat ttggctacag caacagggtg
     5521 gtggacctca tggcccacat ggcctccaag gagggaatgg cgcgtggcgg cgggtccgga
     5581 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc tggcccggcg
     5641 cgccaagtcg acggatccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     5701 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     5761 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     5821 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     5881 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     5941 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     6001 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     6061 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     6121 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     6181 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     6241 acaaccatgg cgcgtatgaa aaagcccgag ctgactgcca cctctgtcga gaagttcctg
     6301 attgaaaagt ttgattccgt gagtgacctg atgcagctga gcgagggcga ggaatcccgg
     6361 gccttctctt ttgacgtggg cggaaggggc tacgtgctga gagtcaactc ttgcgccgac
     6421 ggattctata aggatcgcta cgtgtatcga cactttgcta gtgccgctct gcccatccct
     6481 gaggtgctgg acattggcga gttcagcgaa tccctgacat actgtatctc caggagagcc
     6541 cagggagtga ctctgcagga tctgcctgag accgaactgc ctgccgtgct gcagcctgtg
     6601 gctgaagcta tggacgctat cgcagccgct gatctgtcac agactagcgg gttcggccca
     6661 tttggccccc agggaattgg gcagtacacc acatggagag actttatctg cgcaattgcc
     6721 gatccccacg tgtatcattg gcagacagtc atggacgata ccgtgagcgc cagtgtcgct
     6781 caggcactgg acgagctgat gctgtgggca gaggattgtc ctgaagtgag gcacctggtc
     6841 catgctgact tcgggagcaa caatgtgctg accgataacg gcagaatcac agccgtcatt
     6901 gactggagtg aggctatgtt tggcgattca cagtacgaag tggctaatat cttcttttgg
     6961 cgaccctggc tggcatgcat ggagcagcag acccgctatt tcgagcggcg ccatcctgaa
     7021 ctggcagggt ctccaagact gcgggcctac atgctgcgga ttggcctgga ccagctgtat
     7081 cagagcctgg tggatggaaa ttttgacgat gcagcatggg cacaggggcg gtgcgacgct
     7141 atcgtgcgca gcggagcagg aactgtggga agaacccaga ttgcccgaag gtccgctgca
     7201 gtgtggacag atgggtgcgt ggaggtgctg gcagatagcg gaaataggag accaagtaca
     7261 agaccacggg caaaagagta atctagacgc gtatcgataa gcttagatcg agcacaagag
     7321 gaagagagag accctcactg ctggggagtc cctgccacac tcagtccccc accacactga
     7381 atctcccctc ctcacagttg ccatgtagac cccttgaaga ggggaggggc ctagggagcc
     7441 gcaccttgtc atgtaccatc aataaagtac cctgtgctca accagttact tgtcctgtct
     7501 tattctaggg tctggggcag aggggaggga agctgggctt gtgtcaaggt gagacattct
     7561 tgctggggag ggacctggta tgttctcctc agactgaggg tagggcctcc aaacagcctt
     7621 gcttgcttcg agaaccattt gcttcccgct cagacgtctt gagtgctaca ggaagctggc
     7681 accactactt cagagaacaa ggccttttcc tctcctcgct ccagtcctag gctatctgct
     7741 gttggccaaa catggaagaa gctattctgt gggcagcccc agggaggctg acaggtggag
     7801 gaagtcaggg ctcgcactgg gctctgacgc tgactggtta gtggagctca gcctggagct
     7861 gagctgcagc gggcaattcc agcttggcct ccgcagctgt gaggtcttga gcacgtgctc
     7921 tattgctttc tgtgccctcg tgtcttatct gaggacatcg tggccagccc ctaaggtctt
     7981 caagcaggat tcatctaggt aaaccaagta cctaaaacca tgcccaaggc ggtaaggact
     8041 atataatgtt taaaaatcgg taaaaatgcc cacctcgcat agttttgagg aagatgaact
     8101 gagatgtgtc agggtgactt atttccatca tcgtccttag gggaacttgg gtaggggcaa
     8161 ggcgtgtagc tgggacctag gtccagaccc ctggctctgc cactgaacgg ctcagttgct
     8221 ttgggcagtt actcccgggc ctcactttgc acgtgtgctt acctagtgga gacaaaagta
     8281 catacctcgg tagagcgcgc acgcctgtaa ccccagcact ttgggaggcc aaggtgggtg
     8341 tatcacctga ggtcaggagt ttgagaccag cctggccaac atggtgaaac tccgtctcta
     8401 ctaaaattac aaaaatcagc caggcttcat ggcacatgcc tatagtccca gctacaggca
     8461 tgctgaagca ggagaatcgc ttgcaccccg gaggcagagg ctgcagtgag ctgagaccac
     8521 accactgcac tccagcctag gcaacagagt atgagactcc atctcaaaaa aaaaaaaagt
     8581 acctacctca gagttcaaac tagtgaatat taggaagtgc ttgagacagt gacaccaaag
     8641 tgcacaataa atactcgcca gtttcattat tattaaagaa tccatttgaa tgtcagctca
     8701 acacagcctc ctataccgag gcattgtgaa ccgcatctcc ccagcttctc caggcttttc
     8761 caagaatcag ggacactgta gcctgttggt ctcagtgtat gacagacacg gaggaagcac
     8821 atctttagct gatacttaaa cagagaccct gagcgcacat acacccgcgc acacatgcat
     8881 ggagcttcac cttctctgtc attctgcagt gaccaggaga gcaagagctc ccacctccct
     8941 tcaaaacact gtgcccatcc cgggcactaa ggcctcttta aagcacggca cctccacgag
     9001 ggagggccac agccacatac actccacctg gcaggtggac agcgtgagca cgtggaccat
     9061 agcagggaca aggtgccccg gccagcccca acgccctctg ccgctgacag ggacagaagc
     9121 cctctccagc tgcgtgtgct gcagaggcca tgcgtagcct ccagctgcat tctattccac
     9181 tccagtgcct gggccagtta gcaccagtgt ggaagacagt gagctggctc cggacaacag
     9241 ggatggagga aaggtcccac attcacattc ctgatacgtg gacaaggtga ggggccgcaa
     9301 tcgctctggc agcattttaa agatggggaa gtagcagaca cccacgcgtg aaggcaggag
     9361 agccccaact gtggtggaaa tggccccaga atggtagggc caagcctagc tccagacacc
     9421 ccagagccct ggagaagcca agactgaggg agaaagcctg agggaggagc gccccagtcc
     9481 ccagggaccg gcctggtgca gagctgcagc tgatgttccc ctctgtgcag ccccaccctc
     9541 tgcctcgctg agctccctgc tgcgagggcc tcgggtgcaa gggggaggca ggtctctatc
     9601 tcatggagct gtcagatgag acatcgcgat cggagtcctc agcctcgctt ggcggcggcg
     9661 gcgggtcgct aagcgggacc gcagtgaaag caggagactt tctagaaaaa aacaccagtt
     9721 gtcaaccttg gggcaggcag gaatcctgaa gacggacggc actcctcctc ctgctgcctc
     9781 accctctggc agcccgtgag aagtaccgga agcgagggcg gggccgcggg atggcgaggg
     9841 agcggcaggg actgaactct ctccaaaccc accctgacag ggaaatgggc cccgcctgtg
     9901 tcttgggaac tcagaggctg aggtcaggca tgatggctca cgcctgtaat tccagcactt
     9961 tgggaggcag aggcgggtgg atcacgacgt caggagttca aggcaagcct ggccaacatg
    10021 gtgaaacccc atctctacta aaaatgcaaa aattagccag gtgtggtggc gggcgccttg
    10081 gaggctgagg caagataatc acttgaacct gggaggcgga ggttgtagtg agccaaaaaa
    10141 aaaaaaaaaa gaaatagctg aagtcacagt aggagagaag ctgctgagcc tccagcaccc
    10201 tgactctagg gccttggctt tatgtctatc tgcagtattt ttgtgatttt taaaaattca
    10261 ctttcttgtt gcggtgtaac ttacacaggg tcaaatgcac aaatcatggc cctgacttta
    10321 gataaaaatc tgcccccaca acccttctgt tccttgccag tttttaaact gcctctaacc
    10381 aggggaacca ccagagctgg tggccttggg aggtttcagc cctcccgtca tgaatggaca
    10441 tagctcatcc aactgccaag ggagagagct gtgggtctgg gccagcccca ccaggtaact
    10501 cccaaagggc agccccacag caagatgtga cccagtcatt gcctgagggt ctctggggct
    10561 gtgttccaac ctttctcccc gctgtgtccc cctggaaggc cccatgccca ggggaggcgc
    10621 ctaccggtct ccagactggg tgatgagccg gcggcaggtc tccatctgca cgtccaggcc
    10681 gcgcttcatg ctgcacatct ccatgtactc gtgcaggtgc cggttcatgt cgttcttggc
    10741 cgtggccaac tccagctgtg gtggggcagg cagggccatc ggggttaaca ggtggcgttc
    10801 acagcgcctc tgttgccccc gccaggaggc caacacgcca agagcagtgg ctgggccggg
    10861 ggcccaggca gccattactg aaggctcaga ttttaaaata aaccagacca aggcagaagg
    10921 cagcgaatac accattgttt cctctgcttt tcctggaaat ctttgcctat ggaggaatgc
    10981 aaaggtactg ctgcaggctg tgttctttta agactgataa gagtggtgag gaaagtcagt
    11041 gtgggcggga tggagcagat ccacaggctc ccagggccac acaggttcct atggggcccg
    11101 gctcacccct catcccctgt actgcgctgg tcctgggctg tcccctcact gcactgtgag
    11161 ctcccgtaag ggttagcgac aggttttcct catcctggca gacccagacc tcgaagggaa
    11221 ttaactctct ctgtgacccg tggcatacaa gcctggctaa ctgctgaaag gggcccaatc
    11281 ctcttaccat tctctcctta tcactttatt ttctcaccta aaagccattt tcagacacta
    11341 aaaggaagac tcccatattg gaggtgccat tgattctgag acctgtcctg atttcagaaa
    11401 tgtcacacgt ggaaaacacg gggctcagaa tcaaagaaat ccggtctccc tccccgggcc
    11461 cattctgcag cccacactct gaggcaggcg gagaggcagg gcccaggcag cacaaactca
    11521 gtgccaggga cagtcgatta attaagcgat cgcaggccgc ccgcggtgga gctccaattc
    11581 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
    11641 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    11701 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    11761 cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
    11821 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
    11881 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
    11941 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
    12001 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
    12061 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
    12121 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
    12181 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcag
//
