LOCUS       40924_1024       12005 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GAPTrapT2AIresMeo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12005)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG.
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives
  JOURNAL   Stem Cell Reports (2016) In press
  PUBMED    27594589
REFERENCE   2  (bases 1 to 12005)
  AUTHORS   Stanley EG, Elefanty AG, Kao T, Labonne T.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2016) Cell Biology, Murdoch Childrens Research 
            Institute, Royal Children's Hospital, Flemington Road, Melbourne, 
            Victoria 3052, Australia
REFERENCE   3  (bases 1 to 12005)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12005)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-AUG-2016) Cell Biology, Murdoch Childrens Research Institute, 
            Royal Children's Hospital, Flemington Road, Melbourne, Victoria 
            3052, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    2110..2126
                     /label=lac operator
                     /note="lac operator"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="M13 rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     gene            2216..5559
                     /gene="GAPDH"
                     /label=GAPDH
                     /note="glyceraldehyde-3-phosphate dehydrogenase"
     misc_feature    2219..5556
                     /gene="GAPDH"
                     /label=left arm
                     /note="left arm"
     CDS             5584..5637
                     /codon_start=1
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5660..6246
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6268..7056
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELCGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    7089..11324
                     /label=right arm
                     /note="right arm"
     promoter        complement(11362..11380)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11390..11406)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(11547..12002)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatgnn
     2221 ngggaaggtg aaggtcggag tcaacgggtg agttcgcggg tggctggggg gccctgggct
     2281 gcgaccgccc ccgaaccgcg tctacgagcc ttgcgggctc cgggtctttg cagtcgtatg
     2341 ggggcagggt agctgttccc cgcaaggaga gctcaaggtc agcgctcgga cctggcggag
     2401 ccccgcaccc aggctgtggc gccctgtgca gctccgccct tgcggcgcca tctgcccgga
     2461 gcctccttcc cctagtcccc agaaacagga ggtccctact cccgcccgag atcccgaccc
     2521 ggacccctag gtgggggacg ctttctttcc tttcgcgctc tgcggggtca cgtgtcgcag
     2581 aggagcccct cccccacggc ctccggcacc gcaggccccg ggatgctagt gcgcagcggg
     2641 tgcatccctg tccggatgct gcgcctgcgg tagagcggcc gccatgttgc aaccgggaag
     2701 gaaatgaatg ggcagccgtt aggaaagcct gccggtgact aaccctgcgc tcctgcctcg
     2761 atgggtggag tcgcgtgtgg cggggaagtc aggtggagcg aggctagctg gcccgatttc
     2821 tcctccgggt gatgcttttc ctagattatt ctctggtaaa tcaaagaagt gggtttatgg
     2881 aggtcctctt gtgtcccctc cccgcagagg tgtggtggct gtggcatggt gccaagccgg
     2941 gagaagctga gtcatgggta gttggaaaag gacatttcca ccgcaaaatg gcccctctgg
     3001 tggtggcccc ttcctgcagc gccggctcac ctcacggccc cgcccttccc ctgccagcct
     3061 agcgttgacc cgaccccaaa ggccaggctg taaatgtcac cgggaggatt gggtgtctgg
     3121 gcgcctcggg gaacctgccc ttctccccat tccgtcttcc ggaaaccaga tctcccaccg
     3181 caccctggtc tgaggttaaa tatagctgct gacctttctg tagctggggg cctgggctgg
     3241 ggctctctcc catcccttct ccccacacac atgcacttac ctgtgctccc actcctgatt
     3301 tctggaaaag agctaggaag gacaggcaac ttggcaaatc aaagccctgg gactaggggg
     3361 ttaaaataca gcttcccctc ttcccacccg ccccagtctc tgtccctttt gtaggaggga
     3421 cttagagaag gggtgggctt gccctgtcca gttaatttct gacctttact cctgcccttt
     3481 gagtttgatg atgctgagtg tacaagcgtt ttctccctaa agggtgcagc tgagctaggc
     3541 agcagcaagc attcctgggg tggcatagtg gggtggtgaa taccatgtac aaagcttgtg
     3601 cccagactgt gggtggcagt gccccacatg gccgcttctc ctggaagggc ttcgtatgac
     3661 tgggggtgtt gggcagccct ggagccttca gttgcagcca tgccttaagc caggccagcc
     3721 tggcagggaa gctcaaggga gataaaattc aacctcttgg gccctcctgg gggtaaggag
     3781 atgctgcatt cgccctctta atggggaggt ggcctagggc tgctcacata ttctggagga
     3841 gcctcccctc ctcatgcctt cttgcctctt gtctcttaga tttggtcgta ttgggcgcct
     3901 ggtcaccagg gctgctttta actctggtaa agtggatatt gttgccatca atgacccctt
     3961 cattgacctc aactacatgg tgagtgctac atggtgagcc ccaaagctgg tgtgggagga
     4021 gccacctggc tgatgggcag ccccttcata ccctcacgta ttcccccagg tttacatgtt
     4081 ccaatatgat tccacccatg gcaaattcca tggcaccgtc aaggctgaga acgggaagct
     4141 tgtcatcaat ggaaatccca tcaccatctt ccaggagtga gtggaagaca gaatggaaga
     4201 aatgtgcttt ggggaggcaa ctaggatggt gtggctccct tgggtatatg gtaaccttgt
     4261 gtccctcaat atggtcctgt ccccatctcc cccccacccc cataggcgag atccctccaa
     4321 aatcaagtgg ggcgatgctg gcgctgagta cgtcgtggag tccactggcg tcttcaccac
     4381 catggagaag gctggggtga gtgcaggagg gcccgcggga ggggaagctg actcagccct
     4441 gcaaaggcag gacccgggtt cataactgtc tgcttctctg ctgtaggctc atttgcaggg
     4501 gggagccaaa agggtcatca tctctgcccc ctctgctgat gcccccatgt tcgtcatggg
     4561 tgtgaaccat gagaagtatg acaacagcct caagatcatc aggtgaggaa ggcagggccc
     4621 gtggagaagc ggccagcctg gcaccctatg gacacgctcc cctgacttgc gccccgctcc
     4681 ctctttcttt gcagcaatgc ctcctgcacc accaactgct tagcacccct ggccaaggtc
     4741 atccatgaca actttggtat cgtggaagga ctcatggtat gagagctggg gaatgggact
     4801 gaggctccca cctttctcat ccaagactgg ctcctccctg ccggggctgc gtgcaaccct
     4861 ggggttgggg gttctgggga ctggctttcc cataatttcc tttcaaggtg gggagggagg
     4921 tagaggggtg atgtggggag tacgctgcag ggcctcactc cttttgcaga ccacagtcca
     4981 tgccatcact gccacccaga agactgtgga tggcccctcc gggaaactgt ggcgtgatgg
     5041 ccgcggggct ctccagaaca tcatccctgc ctctactggc gctgccaagg ctgtgggcaa
     5101 ggtcatccct gagctgaacg ggaagctcac tggcatggcc ttccgtgtcc ccactgccaa
     5161 cgtgtcagtg gtggacctga cctgccgtct agaaaaacct gccaaatatg atgacatcaa
     5221 gaaggtggtg aagcaggcgt cggagggccc cctcaagggc atcctgggct acactgagca
     5281 ccaggtggtc tcctctgact tcaacagcga cacccactcc tccacctttg acgctggggc
     5341 tggcattgcc ctcaacgacc actttgtcaa gctcatttcc tggtatgtgg ctggggccag
     5401 agactggctc ttaaaaagtg cagggtctgg cgccctctgg tggctggctc agaaaaaggg
     5461 ccctgacaac tcttttcatc ttctaggtat gacaacgaat ttggctacag caacagggtg
     5521 gtggacctca tggcccacat ggcctccaag gagggaatgg cgcgtggcgg cgggtccgga
     5581 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc tggcccggcg
     5641 cgccaagtcg acggatccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     5701 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     5761 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     5821 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     5881 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     5941 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     6001 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     6061 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     6121 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     6181 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     6241 acaaccatgg cgcgtatggc tcggggcatt gaacaggatg ggctgcacgc cgggtcacct
     6301 gctgcttggg tcgaaagact gtttgggtat gattgggctc agcagacaat cggctgctct
     6361 gacgccgccg tgttcaggct gagtgctcag ggacgccccg tgctgtttgt caagactgac
     6421 ctgtcagggg cactgaacga gctgcaggat gaggcagccc gcctgagctg gctggcaacc
     6481 acaggagtgc cttgtgctgc agtcctggac gtggtcactg aggccggacg agattggctg
     6541 ctgctgggag aagtgccagg acaggacctg ctgagctccc acctggcacc agctgagaag
     6601 gtctccatca tggcagatgc catgaggaga ctgcatactc tggaccccgc cacctgccct
     6661 ttcgatcacc aggctaaaca tcgcattgag cgggctcgca cacgaatgga agcaggcctg
     6721 gtggaccagg acgatctgga tgaggaacac cagggactgg ctcctgcaga gctgtttgca
     6781 aggctgaaag ccagaatgcc agacggcgaa gatctggtgg tcacccatgg agacgcttgc
     6841 ctgcccaaca tcatggtgga gaatggaaga ttcagtgggt ttattgattg tggccgactg
     6901 ggagtcgccg acaggtacca ggatatcgcc ctggctacaa gagacattgc agaggaactg
     6961 tgcggggaat gggccgatcg gttcctggtg ctgtatggca ttgctgctcc cgacagtcag
     7021 aggattgctt tttacaggct gctggacgag ttcttttgaa tctagacgcg tatcgataag
     7081 cttagatcga gcacaagagg aagagagaga ccctcactgc tggggagtcc ctgccacact
     7141 cagtccccca ccacactgaa tctcccctcc tcacagttgc catgtagacc ccttgaagag
     7201 gggaggggcc tagggagccg caccttgtca tgtaccatca ataaagtacc ctgtgctcaa
     7261 ccagttactt gtcctgtctt attctagggt ctggggcaga ggggagggaa gctgggcttg
     7321 tgtcaaggtg agacattctt gctggggagg gacctggtat gttctcctca gactgagggt
     7381 agggcctcca aacagccttg cttgcttcga gaaccatttg cttcccgctc agacgtcttg
     7441 agtgctacag gaagctggca ccactacttc agagaacaag gccttttcct ctcctcgctc
     7501 cagtcctagg ctatctgctg ttggccaaac atggaagaag ctattctgtg ggcagcccca
     7561 gggaggctga caggtggagg aagtcagggc tcgcactggg ctctgacgct gactggttag
     7621 tggagctcag cctggagctg agctgcagcg ggcaattcca gcttggcctc cgcagctgtg
     7681 aggtcttgag cacgtgctct attgctttct gtgccctcgt gtcttatctg aggacatcgt
     7741 ggccagcccc taaggtcttc aagcaggatt catctaggta aaccaagtac ctaaaaccat
     7801 gcccaaggcg gtaaggacta tataatgttt aaaaatcggt aaaaatgccc acctcgcata
     7861 gttttgagga agatgaactg agatgtgtca gggtgactta tttccatcat cgtccttagg
     7921 ggaacttggg taggggcaag gcgtgtagct gggacctagg tccagacccc tggctctgcc
     7981 actgaacggc tcagttgctt tgggcagtta ctcccgggcc tcactttgca cgtgtgctta
     8041 cctagtggag acaaaagtac atacctcggt agagcgcgca cgcctgtaac cccagcactt
     8101 tgggaggcca aggtgggtgt atcacctgag gtcaggagtt tgagaccagc ctggccaaca
     8161 tggtgaaact ccgtctctac taaaattaca aaaatcagcc aggcttcatg gcacatgcct
     8221 atagtcccag ctacaggcat gctgaagcag gagaatcgct tgcaccccgg aggcagaggc
     8281 tgcagtgagc tgagaccaca ccactgcact ccagcctagg caacagagta tgagactcca
     8341 tctcaaaaaa aaaaaaagta cctacctcag agttcaaact agtgaatatt aggaagtgct
     8401 tgagacagtg acaccaaagt gcacaataaa tactcgccag tttcattatt attaaagaat
     8461 ccatttgaat gtcagctcaa cacagcctcc tataccgagg cattgtgaac cgcatctccc
     8521 cagcttctcc aggcttttcc aagaatcagg gacactgtag cctgttggtc tcagtgtatg
     8581 acagacacgg aggaagcaca tctttagctg atacttaaac agagaccctg agcgcacata
     8641 cacccgcgca cacatgcatg gagcttcacc ttctctgtca ttctgcagtg accaggagag
     8701 caagagctcc cacctccctt caaaacactg tgcccatccc gggcactaag gcctctttaa
     8761 agcacggcac ctccacgagg gagggccaca gccacataca ctccacctgg caggtggaca
     8821 gcgtgagcac gtggaccata gcagggacaa ggtgccccgg ccagccccaa cgccctctgc
     8881 cgctgacagg gacagaagcc ctctccagct gcgtgtgctg cagaggccat gcgtagcctc
     8941 cagctgcatt ctattccact ccagtgcctg ggccagttag caccagtgtg gaagacagtg
     9001 agctggctcc ggacaacagg gatggaggaa aggtcccaca ttcacattcc tgatacgtgg
     9061 acaaggtgag gggccgcaat cgctctggca gcattttaaa gatggggaag tagcagacac
     9121 ccacgcgtga aggcaggaga gccccaactg tggtggaaat ggccccagaa tggtagggcc
     9181 aagcctagct ccagacaccc cagagccctg gagaagccaa gactgaggga gaaagcctga
     9241 gggaggagcg ccccagtccc cagggaccgg cctggtgcag agctgcagct gatgttcccc
     9301 tctgtgcagc cccaccctct gcctcgctga gctccctgct gcgagggcct cgggtgcaag
     9361 ggggaggcag gtctctatct catggagctg tcagatgaga catcgcgatc ggagtcctca
     9421 gcctcgcttg gcggcggcgg cgggtcgcta agcgggaccg cagtgaaagc aggagacttt
     9481 ctagaaaaaa acaccagttg tcaaccttgg ggcaggcagg aatcctgaag acggacggca
     9541 ctcctcctcc tgctgcctca ccctctggca gcccgtgaga agtaccggaa gcgagggcgg
     9601 ggccgcggga tggcgaggga gcggcaggga ctgaactctc tccaaaccca ccctgacagg
     9661 gaaatgggcc ccgcctgtgt cttgggaact cagaggctga ggtcaggcat gatggctcac
     9721 gcctgtaatt ccagcacttt gggaggcaga ggcgggtgga tcacgacgtc aggagttcaa
     9781 ggcaagcctg gccaacatgg tgaaacccca tctctactaa aaatgcaaaa attagccagg
     9841 tgtggtggcg ggcgccttgg aggctgaggc aagataatca cttgaacctg ggaggcggag
     9901 gttgtagtga gccaaaaaaa aaaaaaaaag aaatagctga agtcacagta ggagagaagc
     9961 tgctgagcct ccagcaccct gactctaggg ccttggcttt atgtctatct gcagtatttt
    10021 tgtgattttt aaaaattcac tttcttgttg cggtgtaact tacacagggt caaatgcaca
    10081 aatcatggcc ctgactttag ataaaaatct gcccccacaa cccttctgtt ccttgccagt
    10141 ttttaaactg cctctaacca ggggaaccac cagagctggt ggccttggga ggtttcagcc
    10201 ctcccgtcat gaatggacat agctcatcca actgccaagg gagagagctg tgggtctggg
    10261 ccagccccac caggtaactc ccaaagggca gccccacagc aagatgtgac ccagtcattg
    10321 cctgagggtc tctggggctg tgttccaacc tttctccccg ctgtgtcccc ctggaaggcc
    10381 ccatgcccag gggaggcgcc taccggtctc cagactgggt gatgagccgg cggcaggtct
    10441 ccatctgcac gtccaggccg cgcttcatgc tgcacatctc catgtactcg tgcaggtgcc
    10501 ggttcatgtc gttcttggcc gtggccaact ccagctgtgg tggggcaggc agggccatcg
    10561 gggttaacag gtggcgttca cagcgcctct gttgcccccg ccaggaggcc aacacgccaa
    10621 gagcagtggc tgggccgggg gcccaggcag ccattactga aggctcagat tttaaaataa
    10681 accagaccaa ggcagaaggc agcgaataca ccattgtttc ctctgctttt cctggaaatc
    10741 tttgcctatg gaggaatgca aaggtactgc tgcaggctgt gttcttttaa gactgataag
    10801 agtggtgagg aaagtcagtg tgggcgggat ggagcagatc cacaggctcc cagggccaca
    10861 caggttccta tggggcccgg ctcacccctc atcccctgta ctgcgctggt cctgggctgt
    10921 cccctcactg cactgtgagc tcccgtaagg gttagcgaca ggttttcctc atcctggcag
    10981 acccagacct cgaagggaat taactctctc tgtgacccgt ggcatacaag cctggctaac
    11041 tgctgaaagg ggcccaatcc tcttaccatt ctctccttat cactttattt tctcacctaa
    11101 aagccatttt cagacactaa aaggaagact cccatattgg aggtgccatt gattctgaga
    11161 cctgtcctga tttcagaaat gtcacacgtg gaaaacacgg ggctcagaat caaagaaatc
    11221 cggtctccct ccccgggccc attctgcagc ccacactctg aggcaggcgg agaggcaggg
    11281 cccaggcagc acaaactcag tgccagggac agtcgattaa ttaagcgatc gcaggccgcc
    11341 cgcggtggag ctccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
    11401 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    11461 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    11521 agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt aatattttgt taaaattcgc
    11581 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
    11641 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
    11701 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
    11761 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
    11821 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
    11881 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
    11941 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
    12001 gtcag
//
