LOCUS       V016016                 3852 bp    DNA     circular SYN 10-SEP-2020
DEFINITION  Exported.
ACCESSION   V016016
VERSION     V016016
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Engineered systems of inducible anti-repressors for the next
            generation of biological programming
  JOURNAL   Nat Commun 11 (1), 4440 (2020)
   PUBMED   32895374
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3852)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-FEB-2020) Chemical "
FEATURES             Location/Qualifiers
     source          1..3852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        596..673
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             674..1675
                     /gene="cra"
                     /label="Catabolite repressor/activator"
                     /note="Catabolite repressor/activator from Escherichia coli
                     (strain K12). Accession#: P0ACP1"
     protein_bind    1808..1829
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      2020..2564
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        3090..3192
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3193..3849
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
       61 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
      121 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
      181 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga
      241 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggttag
      301 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
      361 atccggaaca taatggtgca gggcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
      421 gccgggggac tgttgggcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
      481 gtcataagtg cggcgacgac accattcctt gcggcggcgg tgctcaacgg cctcaaccta
      541 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac
      601 catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
      661 cagggtggtg aatgtgaaac tggatgaaat cgctcggctg gcgggagtgt cgcggaccac
      721 tgcaagctat gttattaacg gcaaagcgaa gcaataccgt gtgagcgaca aaaccgttga
      781 aaaagtcatg gctgtggtgc gtgagcacaa ttaccacccg aacgccgtgg cagctgggct
      841 tcgtgctgga cgcacacgtt ctattggtct tgtgatcccc gatctggaga acaccagcta
      901 tacccgcatc gctaactatc ttgaacgcca ggcgcggcaa cggggttatc aactgctgcg
      961 tgcctgctca gaagatcagc cagacaacga aatgcggtgc attgagcacc ttttacagcg
     1021 tcaggttgat gccattattg tttcgacgtc gttgcctcct gagcatcctt tttatcaacg
     1081 ctgggctaac gacccgttcc cgattgtcgc gctggaccgc gccctcgatc gtgaacactt
     1141 caccagcgtg gttggtgccg atcaggatga tgccgaaatg ctggcggaag agttacgtaa
     1201 gtttcccgcc gagacggtgc tttatcttgg tgcgctaccg gagctttctg tcagcttcct
     1261 gcgtgaacaa ggtttccgta ctgcctggaa agatgatccg cgcgaagtgc atttcctgta
     1321 tgccaacagc tatgagcggg aggcggctgc ccagttattc gaaaaatggc tggaaacgca
     1381 tccgatgccg caggcgctgt tcacaacgtc gtttgcgttg ttgcaaggag tgatggatgt
     1441 cacgctgcgt cgcgacggca aactgccttc tgacctggca attgccacct ttggcgataa
     1501 cgaactgctc gacttcttac agtgtccggt gctggcagtg gctcaacgtc accgcgatgt
     1561 cgcagagcgt gtgctggaga ttgtcctggc aagcctggac gaaccgcgta agccaaaacc
     1621 tggtttaacg cgcattaaac gtaatctcta tcgccgcggc gtgctcagcc gtagctaact
     1681 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1741 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     1801 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga
     1861 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactaac atgagaatta
     1921 caacttatat cgtatggggc tgacttcagg tgctacattt gaagagataa attgcactga
     1981 aatctagaaa tattttatct gattaataag atgatcttct tgagatcgtt ttggtctgcg
     2041 cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct
     2101 ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact
     2161 tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat
     2221 taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat
     2281 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     2341 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     2401 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg
     2461 ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     2521 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg
     2581 ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc
     2641 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
     2701 agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct
     2761 gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacac
     2821 tccgctagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcagtagctg
     2881 aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa caccatcata
     2941 cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
     3001 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
     3061 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     3121 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     3181 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     3241 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     3301 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     3361 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     3421 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     3481 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     3541 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     3601 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     3661 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     3721 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     3781 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     3841 ggcggggcgt aa
//
