LOCUS       62056_816        5529 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        590..652
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             681..2330
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          2492..2557
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2687..2707
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    3132..3266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3308..3324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3332..3348)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3356..3386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3401..3422)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3710..4298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4472..5329)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5330..5434)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa agggggtaag gccaaagggg
      421 gtaaggccaa agggggcccc ctttggcctt accccctttg gccttacccc ctttggcctt
      481 acctgcatgc ctgcaggtcg actctagagg atccggcccc gcccagcgtc ttgtcattgg
      541 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
      601 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca
      661 acagcgtgcc gcagatctcc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
      721 tctatcctct agaggatgga accgctggag agcaactgca taaggctatg aagagatacg
      781 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg
      841 cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
      901 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
      961 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
     1021 tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc
     1081 aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt
     1141 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
     1201 ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac
     1261 tgataatgaa ttcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa
     1321 ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
     1381 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
     1441 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttt
     1501 tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat
     1561 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
     1621 ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc
     1681 cagggatacg acaaggatat gggctcactg agactacatc agctattctg attacacccg
     1741 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
     1801 tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag
     1861 gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
     1921 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
     1981 tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc cccgctgaat
     2041 tggaatcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca ggtcttcccg
     2101 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
     2161 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
     2221 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
     2281 aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg taaaatgtaa
     2341 ctgtattcag cgatgacgaa attcttagct attgtaatga attcgagctc caccgcggtg
     2401 gcggccgctc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     2461 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     2521 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     2581 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     2641 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     2701 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     2761 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     2821 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     2881 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     2941 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     3001 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     3061 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3121 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3181 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3241 tcaatgtatc ttatcatgtc tggatctcga gggggggccc ggtaccgagc tcgaattcgt
     3301 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3361 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3421 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3901 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4141 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4261 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4321 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4381 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4441 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4501 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4561 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4621 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4681 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4741 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4801 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4861 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4921 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4981 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5041 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5101 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5161 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5221 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5281 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5341 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5461 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     5521 cctttcgtc
//
