LOCUS       V000948                 9477 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000948
VERSION     V000948
KEYWORDS    FUW-tetO-hOCT4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9477)
  AUTHORS   Hockemeyer D, Soldner F, Cook EG, Gao Q, Mitalipova M, Jaenisch R
  TITLE     A drug-inducible system for direct reprogramming of human somatic
            cells to pluripotency.
  JOURNAL   Cell Stem Cell. 2008 Sep 11. 3(3):346-53.
   PUBMED   18786421
REFERENCE   2  (bases 1 to 9477)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9477)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem
            Cell. 2008 Sep 11. 3(3):346-53."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        407..609
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             624..804
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1469..1702
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1887..1931
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2080..2121
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2229..2346
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    2405..2675
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        2708..2745
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     2742..2766
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
     CDS             2851..3930
                     /label="hOct4"
                     /note="Homo sapiens Oct-4 gene. Encodes a transcription
                     factor containing a POU homeodomain that plays a key role
                     in embryonic development and stem cell pluripotency.
                     Aberrant expression in adult tissues is associated with
                     tumorigenesis."
     misc_feature    3958..4546
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(4549..4565)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             5071..5251
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5283..5507
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5553..5981
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5995..6324
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        6372..6419
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             6438..6809
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    6942..7075
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7112..7128)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7136..7152)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7160..7190)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7205..7226)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(7343..7360)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7514..8102)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8276..9133)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9134..9238)
                     /label="AmpR promoter"
     primer_bind     complement(9313..9332)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttaatgat ctcgagttta
     2401 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     2461 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2521 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2581 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     2641 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc
     2701 gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt
     2761 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     2821 tccagcctcc gcggccccga attcgccacc atggcgggac acctggcttc agattttgcc
     2881 ttctcgcccc ctccaggtgg tggaggtgat gggccagggg ggccggagcc gggctgggtt
     2941 gatcctcgga cctggctaag cttccaaggc cctcctggag ggccaggaat cgggccgggg
     3001 gttgggccag gctctgaggt gtgggggatt cccccatgcc ccccgccgta tgagttctgt
     3061 ggggggatgg cgtactgtgg gccccaggtt ggagtggggc tagtgcccca aggcggcttg
     3121 gagacctctc agcctgaggg cgaagcagga gtcggggtgg agagcaactc cgatggggcc
     3181 tccccggagc cctgcaccgt cacccctggt gccgtgaagc tggagaagga gaagctggag
     3241 caaaacccgg aggagtccca ggacatcaaa gctctgcaga aagaactcga gcaatttgcc
     3301 aagctcctga agcagaagag gatcaccctg ggatatacac aggccgatgt ggggctcacc
     3361 ctgggggttc tatttgggaa ggtattcagc caaacgacca tctgccgctt tgaggctctg
     3421 cagcttagct tcaagaacat gtgtaagctg cggcccttgc tgcagaagtg ggtggaggaa
     3481 gctgacaaca atgaaaatct tcaggagata tgcaaagcag aaaccctcgt gcaggcccga
     3541 aagagaaagc gaaccagtat cgagaaccga gtgagaggca acctggagaa tttgttcctg
     3601 cagtgcccga aacccacact gcagcagatc agccacatcg cccagcagct tgggctcgag
     3661 aaggatgtgg tccgagtgtg gttctgtaac cggcgccaga agggcaagcg atcaagcagc
     3721 gactatgcac aacgagagga ttttgaggct gctgggtctc ctttctcagg gggaccagtg
     3781 tcctttcctc tggccccagg gccccatttt ggtaccccag gctatgggag ccctcacttc
     3841 actgcactgt actcctcggt ccctttccct gagggggaag cctttccccc tgtctctgtc
     3901 accactctgg gctctcccat gcattcaaac tgagaattcg atatcaagct tatcgataat
     3961 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4021 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4081 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4141 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4201 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4261 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4321 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     4381 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4441 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4501 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     4561 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     4621 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     4681 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4741 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     4801 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     4861 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     4921 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     4981 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     5041 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     5101 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5161 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5221 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     5281 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5341 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5401 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5461 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5521 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5581 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5641 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5701 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5761 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5821 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5881 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5941 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     6001 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6061 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6121 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6241 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6301 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6361 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     6421 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     6481 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     6541 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     6601 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     6661 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     6721 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     6781 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     6841 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6901 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6961 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     7081 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7261 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7321 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7381 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7921 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9241 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     9301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     9361 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     9421 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcg
//
