LOCUS       V006776                 9785 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V006776
VERSION     V006776
KEYWORDS    FUW-tetO-hMYC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Hockemeyer D, Soldner F, Cook EG, Gao Q, Mitalipova M, Jaenisch R
  TITLE     A drug-inducible system for direct reprogramming of human somatic
            cells to pluripotency.
  JOURNAL   Cell Stem Cell. 2008 Sep 11. 3(3):346-53.
   PUBMED   18786421
REFERENCE   2  (bases 1 to 9785)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9785)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem
            Cell. 2008 Sep 11. 3(3):346-53."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..589
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             604..784
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1449..1682
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1867..1911
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2060..2101
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2209..2326
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    2383..2653
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        2686..2724
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     2721..2745
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
     CDS             2902..4218
                     /label="c-Myc"
                     /note="human c-Myc proto-oncogene"
     misc_feature    4246..4834
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(4837..4853)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             5359..5539
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5571..5795
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5841..6269
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6283..6612
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        6660..6707
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             6726..7097
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    7230..7363
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7400..7416)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7424..7440)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7448..7478)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7493..7514)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(7631..7648)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7802..8390)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8564..9421)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9422..9526)
                     /label="AmpR promoter"
     primer_bind     complement(9601..9620)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
     2701 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
     2821 ttcctctgaa aggctctcct tgcagctgct tagacgctgg atttttttcg ggtagtggaa
     2881 aaccagcagc ctcccgcgac gatgcccctc aacgttagct tcaccaacag gaactatgac
     2941 ctcgactacg actcggtgca gccgtatttc tactgcgacg aggaggagaa cttctaccag
     3001 cagcagcagc agagcgagct gcagcccccg gcgcccagcg aggatatctg gaagaaattc
     3061 gagctgctgc ccaccccgcc cctgtcccct agccgccgct ccgggctctg ctcgccctcc
     3121 tacgttgcgg tcacaccctt ctcccttcgg ggagacaacg acggcggtgg cgggagcttc
     3181 tccacggccg accagctgga gatggtgacc gagctgctgg gaggagacat ggtgaaccag
     3241 agtttcatct gcgacccgga cgacgagacc ttcatcaaaa acatcatcat ccaggactgt
     3301 atgtggagcg gcttctcggc cgccgccaag ctcgtctcag agaagctggc ctcctaccag
     3361 gctgcgcgca aagacagcgg cagcccgaac cccgcccgcg gccacagcgt ctgctccacc
     3421 tccagcttgt acctgcagga tctgagcgcc gccgcctcag agtgcatcga cccctcggtg
     3481 gtcttcccct accctctcaa cgacagcagc tcgcccaagt cctgcgcctc gcaagactcc
     3541 agcgccttct ctccgtcctc ggattctctg ctctcctcga cggagtcctc cccgcagggc
     3601 agccccgagc ccctggtgct ccatgaggag acaccgccca ccaccagcag cgactctgag
     3661 gaggaacaag aagatgagga agaaatcgat gttgtttctg tggaaaagag gcaggctcct
     3721 ggcaaaaggt cagagtctgg atcaccttct gctggaggcc acagcaaacc tcctcacagc
     3781 ccactggtcc tcaagaggtg ccacgtctcc acacatcagc acaactacgc agcgcctccc
     3841 tccactcgga aggactatcc tgctgccaag agggtcaagt tggacagtgt cagagtcctg
     3901 agacagatca gcaacaaccg aaaatgcacc agccccaggt cctcggacac cgaggagaat
     3961 gtcaagaggc gaacacacaa cgtcttggag cgccagagga ggaacgagct aaaacggagc
     4021 ttttttgccc tgcgtgacca gatcccggag ttggaaaaca atgaaaaggc ccccaaggta
     4081 gttatcctta aaaaagccac agcatacatc ctgtccgtcc aagcagagga gcaaaagctc
     4141 atttctgaag aggacttgtt gcggaaacga cgagaacagt tgaaacacaa acttgaacag
     4201 ctacggaact cttgtgcgta agaattcgat atcaagctta tcgataatca acctctggat
     4261 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4321 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4381 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     4441 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4501 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4561 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4621 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     4681 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4741 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4801 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta
     4861 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     4921 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     4981 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     5041 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     5101 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     5161 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     5221 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     5281 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     5341 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5401 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5461 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5521 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     5581 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     5641 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     5701 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     5761 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     5821 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6181 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6241 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     6301 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6361 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6421 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6481 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6541 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6601 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     6661 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     6721 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     6781 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     6841 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     6901 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     6961 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     7021 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     7081 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     7141 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     7201 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     7261 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7321 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     7381 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7441 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7501 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7561 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7621 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7681 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7741 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7801 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7861 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7921 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7981 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8041 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8101 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8161 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8221 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8281 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8341 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8401 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8461 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8521 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8581 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8641 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8701 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8761 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8821 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8881 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8941 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9001 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9061 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9121 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9181 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9241 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9301 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9361 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9421 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9481 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9541 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     9601 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     9661 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     9721 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     9781 atata
//
