LOCUS       V017372                10974 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017372
VERSION     V017372
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2622..3833
                     /label="UbC promoter"
                     /note="human ubiquitin C promoter"
     CDS             4059..4310
                     /label="VC155"
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     misc_feature    5666..6254
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6779..6959
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6991..7215
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7261..7689
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7703..8032
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        8080..8127
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             8146..8517
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    8650..8783
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8868..8898)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8913..8934)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9222..9810)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9984..10841)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10842..10946)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3841 gggctgcagg tcgactctag accaccatga aaaaggttga atccatcatc aggcccgaaa
     3901 agctggagat cgttaagaag gctctctcgg acgctggata tgtgggtatg accgtctctg
     3961 aagtcaaagg ctctggcgtc cagggcggca tcttcgagag gtaccgagga agggtgtact
     4021 ctgcaggcta caacagcgac aacgtctaca tcaccgccga caagcagaag aacggcatca
     4081 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     4141 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4201 gcttccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4261 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggttccg
     4321 gaggcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     4381 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     4441 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc
     4501 ccaccctcgt gaccaccctg ggctacggtc tccaatgctt cgcccgctac cccgaccaca
     4561 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     4621 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     4681 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     4741 ggcacaagct tgagtacaac ggcaccatag tagatctgat ccctaaggta aaaattgaac
     4801 tagtggtgaa ggaggaggac gtcgataacg tgatagacat tatttgcgaa aatgcccgca
     4861 caggtaaccc cggcgacggt aagatatttg tgatcccagt ggagcgagtg gtcagggtgc
     4921 gaaccaaaga ggagggagca tctggtggtg gatccggtgg tggcggtgca tctggtatga
     4981 aaaaggttga agctattatt cgcccagaga aactggagat tgttaagaag gccctgtccg
     5041 acgccggcta tgttggcatg accgtttccg aggttaaagg ccgcggtgcc ggcggtggcg
     5101 acctgattcc taaggtgaag atcgagctgg ttgttaagga ggaagacgtt gataatgtta
     5161 tcgacattat ctgcgagaac gcccgcactg gcaatcctgg tgacggcaaa atcttcgtta
     5221 taccggtcga gcgaattgtg cgagtacgaa ccaaagaaga gggtgcctcc ggcggtggcg
     5281 gtggctccgg tggcgccagc ggcatgaaga aggttgaggc catcatccgc ccagagaagc
     5341 tggaaatcgt taagaaggcc ttaaacgacg acggctacgt cggcatgaca gttagcgagg
     5401 ttaaaggtcg cggtgccggt gggggtgatc tgattcctaa ggttaaaatc gagctcgttg
     5461 ttaaggagga ggacgttgac aacattatcg atatcatctg cgaaaacgct cgcaccggca
     5521 acccaggcga cggcaagatt ttcgtcattc ctgttgagag agtggtgcgt ccccgtacga
     5581 aggaggaggg caaggaagca ctgtgactcg acgaattctt cgaagctagc ggatccgtta
     5641 acaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     5701 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5761 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5821 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5881 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5941 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6001 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6061 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6121 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6181 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6241 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     6301 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     6361 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     6421 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6481 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     6541 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     6601 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     6661 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     6721 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     6781 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6841 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6901 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6961 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7021 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7081 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7141 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7201 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     7261 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7321 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7381 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7441 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7501 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7561 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7621 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7681 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7741 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7801 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7861 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7921 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     7981 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8041 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     8101 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     8161 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     8221 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     8281 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     8341 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     8401 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     8461 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     8521 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     8581 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     8641 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8701 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8761 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     8821 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8881 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8941 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9001 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9061 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9121 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9181 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9241 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9301 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9361 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9421 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9481 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9541 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9601 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9661 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9721 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9781 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9841 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9901 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9961 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10021 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10081 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10141 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10201 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10261 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10321 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10381 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10441 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10501 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10561 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10621 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10681 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10741 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10801 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10861 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10921 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
