LOCUS Exported 10649 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector EN.Cherry, complete sequence. ACCESSION HM771696 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10649) AUTHORS Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA. TITLE Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice JOURNAL Dis Model Mech 4 (4), 537-547 (2011) PUBMED 21324933 REFERENCE 2 (bases 1 to 10649) AUTHORS Chen SX, Osipovich AB, Magnuson MA. TITLE Direct Submission JOURNAL Submitted (15-JUL-2010) Molecular Physiology and Biophysics, Vanderbilt University, 9475 MRB IV, 2214 Garland Avenue, Nashville, TN 37232, USA REFERENCE 3 (bases 1 to 10649) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..10649 /organism="Cloning vector EN.Cherry" /mol_type="other DNA" /db_xref="taxon:1052595" promoter complement(13..31) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(52..68) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 76..92 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(100..130) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 145..166 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(454..1042) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1213..2073) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2074..2178) /gene="bla" /label=AmpR promoter rep_origin complement(2253..2708) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 2850..2866 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 2873..2891 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 2906..2939 /label=Lox66 site /note="Lox66 site" protein_bind complement(2906..2939) /label=lox71 /bound_moiety="Cre recombinase" /note="Left element (LE) mutant of loxP (Araki et al., 2010). Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." regulatory 2957..7037 /regulatory_class="promoter" /note="Rosa 26 promoter" 5'UTR 7043..7101 /label=translational enhancer /note="translational enhancer" 5'UTR 7050..7092 /label=Xenopus globin 5'-UTR /note="translational enhancer from the 5'-UTR of the major beta-globin gene of Xenopus laevis" CDS 7103..7813 /codon_start=1 /product="mCherry" /label=mCherry /note="red fluorescent protein" /protein_id="AEM37564.1" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" regulatory 7827..8712 /regulatory_class="polyA_signal_sequence" /note="rabbit beta globin intron poly A signal" intron 7841..8413 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" polyA_site 8610..8665 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal" misc_feature 8728..8761 /label=FRT site /note="FRT site" protein_bind 8728..8761 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" regulatory 8809..9401 /regulatory_class="promoter" /note="PGK promoter" promoter 8825..9324 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" promoter 9336..9383 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9402..10205 /codon_start=1 /product="Neo" /label=Neo /protein_id="AEM37565.1" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 10219..10520 /regulatory_class="polyA_signal_sequence" /note="rabbit beta globin poly A signal" polyA_signal 10243..10467 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" misc_feature 10525..10558 /label=FRT site /note="FRT site" protein_bind 10525..10558 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" misc_feature complement(10611..10644) /label=Lox2272 site /note="Lox2272 site" protein_bind complement(10611..10644) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites." ORIGIN 1 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct 61 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 121 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 1021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 1081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 1141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 1261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 1321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 1381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 1441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 1501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 1561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 1621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 1681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 1741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 1801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 1861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 1921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 1981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 2041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 2101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 2161 acaaataggg gttccgcgca catttccccg aaaagtgcca caaaaataaa caaatagggg 2221 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa 2281 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 2341 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 2401 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 2461 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 2521 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 2581 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 2641 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 2701 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 2761 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 2821 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga 2881 ctcactatag ggcgaattgg agctcataac ttcgtataat gtatgctata cgaacggtag 2941 cggccgcagg gcccatcgat actgactact attgccttat gtttcaaagt tccaccttca 3001 tcaaaagggt cagaataatt tctaaagtat acaggcttct agatactaag cagtatcaag 3061 atgtaggcat ttttaagtgt gtcagttcaa gtaatccttc taagaaaaaa gcctcttgat 3121 cttccagaga ttactgtaag accacaatca gatccaaaaa aacaggaaat aaaagtggta 3181 gtcattattt tctaagggaa cttgtatatt ataattaagc tcaaaacact tgcaccttaa 3241 ctgtaaatac cttgaactgt aatatcagta tgagtgatat catacttatg ggatacagca 3301 cctaatctac gtaacaaaaa tattattaca accatctaaa acaatcatta acagagtttt 3361 gttttatgga cttgctttta gcactggaaa aacaatatga actacatttg tgaattcaaa 3421 atactctaca atgtttcttc attttttgct acaaattcta gaaccattac atttatggga 3481 tctaaataaa tttgtacaaa attaaacaga ctcccgaagg aaaaggcatt ggttgcagta 3541 ataatcctag ggaggaaaaa ccctacgatt ttatgtgctg cacatgcctg ggtgatggtt 3601 cagtttcaaa tattccttgc cagacaaact acttaaaaag gcttaatcaa agatcaccct 3661 ggttctattt ggttaggggt agggtgggct tagaatggac tcttgctgcc ttcttagtat 3721 cttgaactaa acttactcaa tttctgaata ttccttctgt gtctctgatg gattactgcc 3781 aaaacacatc tttgttcggt ggaaagtcta taatccaaca catacacgat taatccaatt 3841 tataggtcag atatcagtaa gtatatctaa tctggagagg tctacagatt aatttagaat 3901 taacaacaaa ccaagcatta atccaaaagc agctcttttt agtttaggca tccaaatata 3961 tagtaagatg tttcaaagaa catcaatcta ttcacaaagg taaatacggc ctaatacgga 4021 gagacaaata caaatctgaa atgactcaag caattaaaag atggaggaat tcatgtcact 4081 tgcactggga aaacaaaaca aagatcctcc tcaccctatt acccacatta ctatttttag 4141 gttttttagg ggttaaaaaa gatcaaaaca ctaatgaact ttaagtcctg tgaagggtaa 4201 aacctcagat agtaacaaaa agcttccaac ccctcctcaa acaaaaaacc ccaagtcttt 4261 aactttgatc cagttttcag atgctgatat ccataaatgg atacagttat gaattgctaa 4321 ttctggtctc ttcactagca aaaagcaaag cagctcagca gtacaatttc ccaggaaagc 4381 aagcaaggtt tctttccagc ctgagcagcc atcactaagt gcagttccct gcagccaaca 4441 gcattaatgg acgctgcact gctgtccttc cctggagaca gcagccagca ctactcaagc 4501 ttctcacgta gcaaccagag ctccagagcc agcagctgct gccgccttgt atactcactc 4561 ctgtgatcca acacaggagc aaccttttct ttaccccacc cccacttctt aacacacttt 4621 tttttggggg ggggggggaa caagtgctcc atgctggaag gattggaact atgcttttag 4681 aaaggaacaa tcctaaggtc acttttaaat tgaggtcttt gatttgaaaa tcaacaaata 4741 ccaaattcca aatattcgtt ttaattaaac cagcaatgtg gatataagca ttaagtttta 4801 gttttaaaaa ggtcaatttt ccaaacattc agcaatcata tttaaattta cagctaggaa 4861 caagagcctt gggtcatgtc ctaccaaaga acataactca atattctaca catgacaatc 4921 tgaataacct taaagcctct aatcccataa caggccacaa attttggaca gagaactaat 4981 gatcctcctg agaaaactgg aagaaatcca gggaaaagaa attcctgtgt cctccaaact 5041 cagaaatctc taattatgtc agtattctct gctttagtcc taggtcagat tgcacacatc 5101 taaaataacc tcttaaagtt tcctcctagc gacctaaacc attattaata tcaaattaac 5161 catcaaaaca ctttcctctc aatatgctgc acacaaacct cctcctggaa cctcctccat 5221 ctggatcctc cccaatcaaa agtataggta tttaacatat aagcaaggaa gtaatgtaaa 5281 catgaccttg gtcacaaata tgtcatctaa aaacaattta gtcaaggtat ggaggaaatt 5341 cgagaacctg aatcttttta agtattttga gcacaggaac aattggcaaa aggaatccag 5401 gtatagacaa aacccagagc ccagagctct gggcgaaaaa tgagttgctg gtgaagacgt 5461 tacacaagta acatgagaaa gcagaaaatg caggtcatcc acgcacccct gacccaggcc 5521 agcagggcgg gctgcagcat cagtacacag gagaaagatc cttattccta agaatgagaa 5581 aggcaaaggc gcccgataga ataaattagc atagaagggg ctttcccagg agttaaaact 5641 ttccttctga gcgattacct actaaaacca gggcttttgc ccactaccat ttacctagga 5701 tcttggcttg cacggattca taggggcata tccctccccc tcttctttag agtcgttctt 5761 aaaagatcgc tctccacgcc ctaggcaggg aaaacgacaa aatctggctc aattccaggc 5821 tagaacccta caaattcaac agggatatcg caaggatact ggggcatacg ccacagggag 5881 tccaagaatg tgaggtgggg gtggcgaagg taatgtcttt ggtgtgggaa aagcagcagc 5941 catctgagat aggaactgga aaaccagagg agaggcgttc aggaagatta tggaggggag 6001 gactgggccc ccacgagcga ccagagttgt cacaaggccg caagaacagg ggaggtgggg 6061 ggctcaggga cagaaaaaaa agtatgtgta ttttgagagc agggttggga ggcctctcct 6121 gaaaagggta taaacgtgga gtaggcaata cccaggcaaa aaggggagac cagagtaggg 6181 ggaggggaag agtcctgacc cagggaagac attaaaaagg tagtggggtc gactagatga 6241 aggagagcct ttctctctgg gcaagagcgg tgcaatggtg tgtaaaggta gctgagaaga 6301 cgaaaagggc aagcatcttc ctgctaccag gctggggagg cccaggccca cgaccccgag 6361 gagagggaac gcagggagac tgaggtgacc cttctttccc ccggggcccg gtcgtgtggt 6421 tcggtgtctc ttttctgttg gacccttacc ttgacccagg cgctgccggg gcctgggccc 6481 gggctgcggc gcacggcact cccgggaggc agcgagactc gagttaggcc caacgcggcg 6541 ccacggcgtt tcctggccgg gaatggcccg tacccgtgag gtgggggtgg ggggcagaaa 6601 aggcggagcg agcccgaggc ggggaggggg agggccaggg gcggaggggg ccggcactac 6661 tgtgttggcg gactggcggg actagggctg cgtgagtctc tgagcgcagc gggcggcggc 6721 cgcccctccc ccggcggcgg cagcggcggc agcggcggca gctcactcag cccgctgccc 6781 gagcggaaac gccactgacc gcacggggat tcccagtgcc ggcgccaggg gcacgcggga 6841 cacgccccct cccgccgcgc cattggcctc tccgcccacc gccccacact tattggccgg 6901 tgcgccgcca atcagcggag gctgccgggg ccgcctaaag aagaggctgt gctttggggc 6961 tccggctcct cagagagcct cggctaggta ggggatcggg actctggcgg gagggcggct 7021 tggtgcgttt gcggggacgc gtaagcttgc ttgttctttt tgcagaagct cagaataaac 7081 gctcaacttt ggcagatcta ccatggtgag caagggcgag gaggataaca tggccatcat 7141 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga 7201 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 7261 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg 7321 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc 7381 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac 7441 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa 7501 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga 7561 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa 7621 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca 7681 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta 7741 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga 7801 gctgtacaag taaggatcct ctagagctga gaacttcagg gtgagtttgg ggacccttga 7861 ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag 7921 ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg 7981 tttctttcac tttctactct gttgacaacc attgtctcct cttattttct tttcattttc 8041 tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt 8101 ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat 8161 attatattgt acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag 8221 aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta 8281 caccctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat 8341 gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt 8401 ctctttccta cagctcctgg gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa 8461 agaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc 8521 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat 8581 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt 8641 gtgttggaat tttttgtgtc tctcactcgg aaggacataa accaattgtt cgaacgtacg 8701 tcgcgactcg aggcggccgc gaattccgaa gttcctattc tctagaaagt ataggaactt 8761 caggtctgaa gaggagttta cgtccagcca agctagcttg gctgcaggtc gtcgaaattc 8821 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg 8881 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 8941 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta 9001 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta 9061 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc 9121 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga 9181 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg 9241 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc 9301 tcttcctcat ctccgggcct ttcgacctgc agcctgttga caattaatca tcggcatagt 9361 atatcggcat agtataatac gacaaggtga ggaactaaac catgggatcg gccattgaac 9421 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 9481 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 9541 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 9601 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 9661 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 9721 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 9781 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 9841 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 9901 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgatgatc 9961 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 10021 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 10081 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 10141 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 10201 tctgagggga tcaattctct agagctcgct gatcagcctc gactgtgcct tctagttgcc 10261 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 10321 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 10381 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 10441 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga 10501 ctagagcttg cggaaccctt cgaagaagtt cctattctct agaaagtata ggaacttcat 10561 gcatgtttaa accgccggcg gttaacccgc ggaagcttac gcgtctcgag ataacttcgt 10621 ataaagtatc ctatacgaag ttatggtac //