LOCUS Exported 10658 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector EN.CFP, complete sequence. ACCESSION HM771698 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10658) AUTHORS Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA. TITLE Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice JOURNAL Dis Model Mech 4 (4), 537-547 (2011) PUBMED 21324933 REFERENCE 2 (bases 1 to 10658) AUTHORS Chen SX, Osipovich AB, Magnuson MA. TITLE Direct Submission JOURNAL Submitted (16-JUL-2010) Molecular Physiology and Biophysics, Vanderbilt University, 9475 MRB IV, 2214 Garland Avenue, Nashville, TN 37232, USA REFERENCE 3 (bases 1 to 10658) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..10658 /organism="Cloning vector EN.CFP" /mol_type="other DNA" /db_xref="taxon:1052597" promoter complement(13..31) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(52..68) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 76..92 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(100..130) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 145..166 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(454..1042) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1213..2073) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2074..2178) /gene="bla" /label=AmpR promoter rep_origin complement(2253..2708) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 2850..2866 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 2873..2891 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 2906..2939 /label=Lox66 site /note="Lox66 site" protein_bind complement(2906..2939) /label=lox71 /bound_moiety="Cre recombinase" /note="Left element (LE) mutant of loxP (Araki et al., 2010). Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." regulatory 2957..7037 /regulatory_class="promoter" /note="Rosa 26 promoter" 5'UTR 7043..7101 /label=translational enhancer /note="translational enhancer" 5'UTR 7050..7092 /label=Xenopus globin 5'-UTR /note="translational enhancer from the 5'-UTR of the major beta-globin gene of Xenopus laevis" CDS 7103..7822 /codon_start=1 /product="CFP" /label=CFP /note="cyan fluorescent protein cerulean variant" /protein_id="AEM37568.1" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" regulatory 7836..8721 /regulatory_class="polyA_signal_sequence" /note="rabbit beta globin intron poly A signal" intron 7850..8422 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" polyA_site 8619..8674 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal" misc_feature 8737..8770 /label=FRT site /note="FRT site" protein_bind 8737..8770 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" regulatory 8818..9410 /regulatory_class="promoter" /note="PGK promoter" promoter 8834..9333 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" promoter 9345..9392 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9411..10214 /codon_start=1 /product="Neo" /label=Neo /protein_id="AEM37569.1" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 10228..10529 /regulatory_class="polyA_signal_sequence" /note="rabbit beta globin poly A signal" polyA_signal 10252..10476 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" misc_feature 10534..10567 /label=FRT site /note="FRT site" protein_bind 10534..10567 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" misc_feature complement(10620..10653) /label=Lox2272 site /note="Lox2272 site" protein_bind complement(10620..10653) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites." ORIGIN 1 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct 61 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 121 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 1021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 1081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 1141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 1261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 1321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 1381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 1441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 1501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 1561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 1621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 1681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 1741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 1801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 1861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 1921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 1981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 2041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 2101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 2161 acaaataggg gttccgcgca catttccccg aaaagtgcca caaaaataaa caaatagggg 2221 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa 2281 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 2341 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 2401 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 2461 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 2521 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 2581 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 2641 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 2701 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 2761 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 2821 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga 2881 ctcactatag ggcgaattgg agctcataac ttcgtataat gtatgctata cgaacggtag 2941 cggccgcagg gcccatcgat actgactact attgccttat gtttcaaagt tccaccttca 3001 tcaaaagggt cagaataatt tctaaagtat acaggcttct agatactaag cagtatcaag 3061 atgtaggcat ttttaagtgt gtcagttcaa gtaatccttc taagaaaaaa gcctcttgat 3121 cttccagaga ttactgtaag accacaatca gatccaaaaa aacaggaaat aaaagtggta 3181 gtcattattt tctaagggaa cttgtatatt ataattaagc tcaaaacact tgcaccttaa 3241 ctgtaaatac cttgaactgt aatatcagta tgagtgatat catacttatg ggatacagca 3301 cctaatctac gtaacaaaaa tattattaca accatctaaa acaatcatta acagagtttt 3361 gttttatgga cttgctttta gcactggaaa aacaatatga actacatttg tgaattcaaa 3421 atactctaca atgtttcttc attttttgct acaaattcta gaaccattac atttatggga 3481 tctaaataaa tttgtacaaa attaaacaga ctcccgaagg aaaaggcatt ggttgcagta 3541 ataatcctag ggaggaaaaa ccctacgatt ttatgtgctg cacatgcctg ggtgatggtt 3601 cagtttcaaa tattccttgc cagacaaact acttaaaaag gcttaatcaa agatcaccct 3661 ggttctattt ggttaggggt agggtgggct tagaatggac tcttgctgcc ttcttagtat 3721 cttgaactaa acttactcaa tttctgaata ttccttctgt gtctctgatg gattactgcc 3781 aaaacacatc tttgttcggt ggaaagtcta taatccaaca catacacgat taatccaatt 3841 tataggtcag atatcagtaa gtatatctaa tctggagagg tctacagatt aatttagaat 3901 taacaacaaa ccaagcatta atccaaaagc agctcttttt agtttaggca tccaaatata 3961 tagtaagatg tttcaaagaa catcaatcta ttcacaaagg taaatacggc ctaatacgga 4021 gagacaaata caaatctgaa atgactcaag caattaaaag atggaggaat tcatgtcact 4081 tgcactggga aaacaaaaca aagatcctcc tcaccctatt acccacatta ctatttttag 4141 gttttttagg ggttaaaaaa gatcaaaaca ctaatgaact ttaagtcctg tgaagggtaa 4201 aacctcagat agtaacaaaa agcttccaac ccctcctcaa acaaaaaacc ccaagtcttt 4261 aactttgatc cagttttcag atgctgatat ccataaatgg atacagttat gaattgctaa 4321 ttctggtctc ttcactagca aaaagcaaag cagctcagca gtacaatttc ccaggaaagc 4381 aagcaaggtt tctttccagc ctgagcagcc atcactaagt gcagttccct gcagccaaca 4441 gcattaatgg acgctgcact gctgtccttc cctggagaca gcagccagca ctactcaagc 4501 ttctcacgta gcaaccagag ctccagagcc agcagctgct gccgccttgt atactcactc 4561 ctgtgatcca acacaggagc aaccttttct ttaccccacc cccacttctt aacacacttt 4621 tttttggggg ggggggggaa caagtgctcc atgctggaag gattggaact atgcttttag 4681 aaaggaacaa tcctaaggtc acttttaaat tgaggtcttt gatttgaaaa tcaacaaata 4741 ccaaattcca aatattcgtt ttaattaaac cagcaatgtg gatataagca ttaagtttta 4801 gttttaaaaa ggtcaatttt ccaaacattc agcaatcata tttaaattta cagctaggaa 4861 caagagcctt gggtcatgtc ctaccaaaga acataactca atattctaca catgacaatc 4921 tgaataacct taaagcctct aatcccataa caggccacaa attttggaca gagaactaat 4981 gatcctcctg agaaaactgg aagaaatcca gggaaaagaa attcctgtgt cctccaaact 5041 cagaaatctc taattatgtc agtattctct gctttagtcc taggtcagat tgcacacatc 5101 taaaataacc tcttaaagtt tcctcctagc gacctaaacc attattaata tcaaattaac 5161 catcaaaaca ctttcctctc aatatgctgc acacaaacct cctcctggaa cctcctccat 5221 ctggatcctc cccaatcaaa agtataggta tttaacatat aagcaaggaa gtaatgtaaa 5281 catgaccttg gtcacaaata tgtcatctaa aaacaattta gtcaaggtat ggaggaaatt 5341 cgagaacctg aatcttttta agtattttga gcacaggaac aattggcaaa aggaatccag 5401 gtatagacaa aacccagagc ccagagctct gggcgaaaaa tgagttgctg gtgaagacgt 5461 tacacaagta acatgagaaa gcagaaaatg caggtcatcc acgcacccct gacccaggcc 5521 agcagggcgg gctgcagcat cagtacacag gagaaagatc cttattccta agaatgagaa 5581 aggcaaaggc gcccgataga ataaattagc atagaagggg ctttcccagg agttaaaact 5641 ttccttctga gcgattacct actaaaacca gggcttttgc ccactaccat ttacctagga 5701 tcttggcttg cacggattca taggggcata tccctccccc tcttctttag agtcgttctt 5761 aaaagatcgc tctccacgcc ctaggcaggg aaaacgacaa aatctggctc aattccaggc 5821 tagaacccta caaattcaac agggatatcg caaggatact ggggcatacg ccacagggag 5881 tccaagaatg tgaggtgggg gtggcgaagg taatgtcttt ggtgtgggaa aagcagcagc 5941 catctgagat aggaactgga aaaccagagg agaggcgttc aggaagatta tggaggggag 6001 gactgggccc ccacgagcga ccagagttgt cacaaggccg caagaacagg ggaggtgggg 6061 ggctcaggga cagaaaaaaa agtatgtgta ttttgagagc agggttggga ggcctctcct 6121 gaaaagggta taaacgtgga gtaggcaata cccaggcaaa aaggggagac cagagtaggg 6181 ggaggggaag agtcctgacc cagggaagac attaaaaagg tagtggggtc gactagatga 6241 aggagagcct ttctctctgg gcaagagcgg tgcaatggtg tgtaaaggta gctgagaaga 6301 cgaaaagggc aagcatcttc ctgctaccag gctggggagg cccaggccca cgaccccgag 6361 gagagggaac gcagggagac tgaggtgacc cttctttccc ccggggcccg gtcgtgtggt 6421 tcggtgtctc ttttctgttg gacccttacc ttgacccagg cgctgccggg gcctgggccc 6481 gggctgcggc gcacggcact cccgggaggc agcgagactc gagttaggcc caacgcggcg 6541 ccacggcgtt tcctggccgg gaatggcccg tacccgtgag gtgggggtgg ggggcagaaa 6601 aggcggagcg agcccgaggc ggggaggggg agggccaggg gcggaggggg ccggcactac 6661 tgtgttggcg gactggcggg actagggctg cgtgagtctc tgagcgcagc gggcggcggc 6721 cgcccctccc ccggcggcgg cagcggcggc agcggcggca gctcactcag cccgctgccc 6781 gagcggaaac gccactgacc gcacggggat tcccagtgcc ggcgccaggg gcacgcggga 6841 cacgccccct cccgccgcgc cattggcctc tccgcccacc gccccacact tattggccgg 6901 tgcgccgcca atcagcggag gctgccgggg ccgcctaaag aagaggctgt gctttggggc 6961 tccggctcct cagagagcct cggctaggta ggggatcggg actctggcgg gagggcggct 7021 tggtgcgttt gcggggacgc gtaagcttgc ttgttctttt tgcagaagct cagaataaac 7081 gctcaacttt ggcagatcta ccatggtgag caagggcgag gagctgttca ccggggtggt 7141 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 7201 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 7261 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcgc 7321 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 7381 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 7441 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 7501 ggacggcaac atcctggggc acaagctgga gtacaacgcc atcagcgaca acgtctatat 7561 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga 7621 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 7681 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa 7741 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 7801 catggacgag ctgtacaagt aaggatcctc tagagctgag aacttcaggg tgagtttggg 7861 gacccttgat tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa 7921 agttttcagg gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg 7981 ataattttgt ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt 8041 ttcattttct gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt 8101 cacttttgtt tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa 8161 tcagggtata ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg 8221 ataaggtaga atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag 8281 aaacaactac accctggtca tcatcctgcc tttctcttta tggttacaat gatatacact 8341 gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca 8401 tgccttcttc tctttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca 8461 ttttggcaaa gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt 8521 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg 8581 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat 8641 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacataaa ccaattgttc 8701 gaacgtacgt cgcgactcga ggcggccgcg aattccgaag ttcctattct ctagaaagta 8761 taggaacttc aggtctgaag aggagtttac gtccagccaa gctagcttgg ctgcaggtcg 8821 tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 8881 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca 8941 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact 9001 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa 9061 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag 9121 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag 9181 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc 9241 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg 9301 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcctgttgac aattaatcat 9361 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atgggatcgg 9421 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 9481 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 9541 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 9601 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 9661 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 9721 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 9781 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 9841 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 9901 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 9961 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 10021 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 10081 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 10141 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 10201 acgagttctt ctgaggggat caattctcta gagctcgctg atcagcctcg actgtgcctt 10261 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 10321 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 10381 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 10441 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 10501 ggggctcgac tagagcttgc ggaacccttc gaagaagttc ctattctcta gaaagtatag 10561 gaacttcatg catgtttaaa ccgccggcgg ttaacccgcg gaagcttacg cgtctcgaga 10621 taacttcgta taaagtatcc tatacgaagt tatggtac //