LOCUS       Exported               10649 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector EN.Apple, complete sequence.
ACCESSION   HM771700
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, 
            Yuan W, Piston DW, Magnuson MA.
  TITLE     Quantification of factors influencing fluorescent protein expression
            using RMCE to generate an allelic series in the ROSA26 locus in mice
  JOURNAL   Dis Model Mech 4 (4), 537-547 (2011)
  PUBMED    21324933
REFERENCE   2  (bases 1 to 10649)
  AUTHORS   Chen SX, Osipovich AB, Magnuson MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUL-2010) Molecular Physiology and Biophysics, 
            Vanderbilt University, 9475 MRB IV, 2214 Garland Avenue, Nashville, 
            TN 37232, USA
REFERENCE   3  (bases 1 to 10649)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Cloning vector EN.Apple"
                     /mol_type="other DNA"
                     /db_xref="taxon:1052599"
     promoter        complement(13..31)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(52..68)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    76..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(100..130)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    145..166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(454..1042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1213..2073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2074..2178)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2253..2708)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     2850..2866
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2873..2891
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2906..2939
                     /label=Lox66 site
                     /note="Lox66 site"
     protein_bind    complement(2906..2939)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      2957..7037
                     /regulatory_class="promoter"
                     /note="Rosa 26 promoter"
     5'UTR           7043..7101
                     /label=translational enhancer
                     /note="translational enhancer"
     5'UTR           7050..7092
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             7103..7813
                     /codon_start=1
                     /product="mApple"
                     /label=mApple
                     /note="red fluorescent protein"
                     /protein_id="AEM37572.1"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     regulatory      7827..8712
                     /regulatory_class="polyA_signal_sequence"
                     /note="rabbit beta globin intron poly A signal"
     intron          7841..8413
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      8610..8665
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    8728..8761
                     /label=FRT site
                     /note="FRT site"
     protein_bind    8728..8761
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      8809..9401
                     /regulatory_class="promoter"
                     /note="PGK promoter"
     promoter        8825..9324
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        9336..9383
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9402..10205
                     /codon_start=1
                     /product="Neo"
                     /label=Neo
                     /protein_id="AEM37573.1"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      10219..10520
                     /regulatory_class="polyA_signal_sequence"
                     /note="rabbit beta globin poly A signal"
     polyA_signal    10243..10467
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    10525..10558
                     /label=FRT site
                     /note="FRT site"
     protein_bind    10525..10558
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    complement(10611..10644)
                     /label=Lox2272 site
                     /note="Lox2272 site"
     protein_bind    complement(10611..10644)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
ORIGIN
        1 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
       61 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      121 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
      901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2161 acaaataggg gttccgcgca catttccccg aaaagtgcca caaaaataaa caaatagggg
     2221 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     2281 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     2341 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     2401 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     2461 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     2521 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     2581 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     2641 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     2701 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     2761 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     2821 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     2881 ctcactatag ggcgaattgg agctcataac ttcgtataat gtatgctata cgaacggtag
     2941 cggccgcagg gcccatcgat actgactact attgccttat gtttcaaagt tccaccttca
     3001 tcaaaagggt cagaataatt tctaaagtat acaggcttct agatactaag cagtatcaag
     3061 atgtaggcat ttttaagtgt gtcagttcaa gtaatccttc taagaaaaaa gcctcttgat
     3121 cttccagaga ttactgtaag accacaatca gatccaaaaa aacaggaaat aaaagtggta
     3181 gtcattattt tctaagggaa cttgtatatt ataattaagc tcaaaacact tgcaccttaa
     3241 ctgtaaatac cttgaactgt aatatcagta tgagtgatat catacttatg ggatacagca
     3301 cctaatctac gtaacaaaaa tattattaca accatctaaa acaatcatta acagagtttt
     3361 gttttatgga cttgctttta gcactggaaa aacaatatga actacatttg tgaattcaaa
     3421 atactctaca atgtttcttc attttttgct acaaattcta gaaccattac atttatggga
     3481 tctaaataaa tttgtacaaa attaaacaga ctcccgaagg aaaaggcatt ggttgcagta
     3541 ataatcctag ggaggaaaaa ccctacgatt ttatgtgctg cacatgcctg ggtgatggtt
     3601 cagtttcaaa tattccttgc cagacaaact acttaaaaag gcttaatcaa agatcaccct
     3661 ggttctattt ggttaggggt agggtgggct tagaatggac tcttgctgcc ttcttagtat
     3721 cttgaactaa acttactcaa tttctgaata ttccttctgt gtctctgatg gattactgcc
     3781 aaaacacatc tttgttcggt ggaaagtcta taatccaaca catacacgat taatccaatt
     3841 tataggtcag atatcagtaa gtatatctaa tctggagagg tctacagatt aatttagaat
     3901 taacaacaaa ccaagcatta atccaaaagc agctcttttt agtttaggca tccaaatata
     3961 tagtaagatg tttcaaagaa catcaatcta ttcacaaagg taaatacggc ctaatacgga
     4021 gagacaaata caaatctgaa atgactcaag caattaaaag atggaggaat tcatgtcact
     4081 tgcactggga aaacaaaaca aagatcctcc tcaccctatt acccacatta ctatttttag
     4141 gttttttagg ggttaaaaaa gatcaaaaca ctaatgaact ttaagtcctg tgaagggtaa
     4201 aacctcagat agtaacaaaa agcttccaac ccctcctcaa acaaaaaacc ccaagtcttt
     4261 aactttgatc cagttttcag atgctgatat ccataaatgg atacagttat gaattgctaa
     4321 ttctggtctc ttcactagca aaaagcaaag cagctcagca gtacaatttc ccaggaaagc
     4381 aagcaaggtt tctttccagc ctgagcagcc atcactaagt gcagttccct gcagccaaca
     4441 gcattaatgg acgctgcact gctgtccttc cctggagaca gcagccagca ctactcaagc
     4501 ttctcacgta gcaaccagag ctccagagcc agcagctgct gccgccttgt atactcactc
     4561 ctgtgatcca acacaggagc aaccttttct ttaccccacc cccacttctt aacacacttt
     4621 tttttggggg ggggggggaa caagtgctcc atgctggaag gattggaact atgcttttag
     4681 aaaggaacaa tcctaaggtc acttttaaat tgaggtcttt gatttgaaaa tcaacaaata
     4741 ccaaattcca aatattcgtt ttaattaaac cagcaatgtg gatataagca ttaagtttta
     4801 gttttaaaaa ggtcaatttt ccaaacattc agcaatcata tttaaattta cagctaggaa
     4861 caagagcctt gggtcatgtc ctaccaaaga acataactca atattctaca catgacaatc
     4921 tgaataacct taaagcctct aatcccataa caggccacaa attttggaca gagaactaat
     4981 gatcctcctg agaaaactgg aagaaatcca gggaaaagaa attcctgtgt cctccaaact
     5041 cagaaatctc taattatgtc agtattctct gctttagtcc taggtcagat tgcacacatc
     5101 taaaataacc tcttaaagtt tcctcctagc gacctaaacc attattaata tcaaattaac
     5161 catcaaaaca ctttcctctc aatatgctgc acacaaacct cctcctggaa cctcctccat
     5221 ctggatcctc cccaatcaaa agtataggta tttaacatat aagcaaggaa gtaatgtaaa
     5281 catgaccttg gtcacaaata tgtcatctaa aaacaattta gtcaaggtat ggaggaaatt
     5341 cgagaacctg aatcttttta agtattttga gcacaggaac aattggcaaa aggaatccag
     5401 gtatagacaa aacccagagc ccagagctct gggcgaaaaa tgagttgctg gtgaagacgt
     5461 tacacaagta acatgagaaa gcagaaaatg caggtcatcc acgcacccct gacccaggcc
     5521 agcagggcgg gctgcagcat cagtacacag gagaaagatc cttattccta agaatgagaa
     5581 aggcaaaggc gcccgataga ataaattagc atagaagggg ctttcccagg agttaaaact
     5641 ttccttctga gcgattacct actaaaacca gggcttttgc ccactaccat ttacctagga
     5701 tcttggcttg cacggattca taggggcata tccctccccc tcttctttag agtcgttctt
     5761 aaaagatcgc tctccacgcc ctaggcaggg aaaacgacaa aatctggctc aattccaggc
     5821 tagaacccta caaattcaac agggatatcg caaggatact ggggcatacg ccacagggag
     5881 tccaagaatg tgaggtgggg gtggcgaagg taatgtcttt ggtgtgggaa aagcagcagc
     5941 catctgagat aggaactgga aaaccagagg agaggcgttc aggaagatta tggaggggag
     6001 gactgggccc ccacgagcga ccagagttgt cacaaggccg caagaacagg ggaggtgggg
     6061 ggctcaggga cagaaaaaaa agtatgtgta ttttgagagc agggttggga ggcctctcct
     6121 gaaaagggta taaacgtgga gtaggcaata cccaggcaaa aaggggagac cagagtaggg
     6181 ggaggggaag agtcctgacc cagggaagac attaaaaagg tagtggggtc gactagatga
     6241 aggagagcct ttctctctgg gcaagagcgg tgcaatggtg tgtaaaggta gctgagaaga
     6301 cgaaaagggc aagcatcttc ctgctaccag gctggggagg cccaggccca cgaccccgag
     6361 gagagggaac gcagggagac tgaggtgacc cttctttccc ccggggcccg gtcgtgtggt
     6421 tcggtgtctc ttttctgttg gacccttacc ttgacccagg cgctgccggg gcctgggccc
     6481 gggctgcggc gcacggcact cccgggaggc agcgagactc gagttaggcc caacgcggcg
     6541 ccacggcgtt tcctggccgg gaatggcccg tacccgtgag gtgggggtgg ggggcagaaa
     6601 aggcggagcg agcccgaggc ggggaggggg agggccaggg gcggaggggg ccggcactac
     6661 tgtgttggcg gactggcggg actagggctg cgtgagtctc tgagcgcagc gggcggcggc
     6721 cgcccctccc ccggcggcgg cagcggcggc agcggcggca gctcactcag cccgctgccc
     6781 gagcggaaac gccactgacc gcacggggat tcccagtgcc ggcgccaggg gcacgcggga
     6841 cacgccccct cccgccgcgc cattggcctc tccgcccacc gccccacact tattggccgg
     6901 tgcgccgcca atcagcggag gctgccgggg ccgcctaaag aagaggctgt gctttggggc
     6961 tccggctcct cagagagcct cggctaggta ggggatcggg actctggcgg gagggcggct
     7021 tggtgcgttt gcggggacgc gtaagcttgc ttgttctttt tgcagaagct cagaataaac
     7081 gctcaacttt ggcagatcta ccatggtgag caagggcgag gagaataaca tggccatcat
     7141 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     7201 gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta agctgaaggt
     7261 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     7321 ctccaaggtc tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc
     7381 cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta ttcacgttaa
     7441 ccaggactcc tccctgcagg acggcgtgtt catctacaag gtgaagctgc gcggcaccaa
     7501 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccgagga
     7561 gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaagaggc tgaagctgaa
     7621 ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca
     7681 gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta
     7741 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     7801 gctgtacaag taaggatcct ctagagctga gaacttcagg gtgagtttgg ggacccttga
     7861 ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag
     7921 ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg
     7981 tttctttcac tttctactct gttgacaacc attgtctcct cttattttct tttcattttc
     8041 tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt
     8101 ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat
     8161 attatattgt acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag
     8221 aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta
     8281 caccctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat
     8341 gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt
     8401 ctctttccta cagctcctgg gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa
     8461 agaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     8521 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     8581 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     8641 gtgttggaat tttttgtgtc tctcactcgg aaggacataa accaattgtt cgaacgtacg
     8701 tcgcgactcg aggcggccgc gaattccgaa gttcctattc tctagaaagt ataggaactt
     8761 caggtctgaa gaggagttta cgtccagcca agctagcttg gctgcaggtc gtcgaaattc
     8821 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     8881 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     8941 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     9001 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     9061 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     9121 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     9181 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     9241 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     9301 tcttcctcat ctccgggcct ttcgacctgc agcctgttga caattaatca tcggcatagt
     9361 atatcggcat agtataatac gacaaggtga ggaactaaac catgggatcg gccattgaac
     9421 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     9481 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     9541 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     9601 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     9661 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     9721 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     9781 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     9841 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     9901 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgatgatc
     9961 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
    10021 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
    10081 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
    10141 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
    10201 tctgagggga tcaattctct agagctcgct gatcagcctc gactgtgcct tctagttgcc
    10261 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
    10321 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
    10381 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
    10441 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
    10501 ctagagcttg cggaaccctt cgaagaagtt cctattctct agaaagtata ggaacttcat
    10561 gcatgtttaa accgccggcg gttaacccgc ggaagcttac gcgtctcgag ataacttcgt
    10621 ataaagtatc ctatacgaag ttatggtac
//
