LOCUS 40924_755 8725 bp DNA circular SYN 13-MAY-2021 DEFINITION Retroviral construct for expressing human EGFR in human cells. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8725) AUTHORS Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M, Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M TITLE Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants. JOURNAL PLoS Med. 2005 Oct 4. 2(11):e313. PUBMED 16187797 REFERENCE 2 (bases 1 to 8725) TITLE Direct Submission REFERENCE 3 (bases 1 to 8725) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Med. 2005 Oct 4. 2(11):e313." COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..8725 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 23..492 /label=LTR /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 557..756 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 957..1373 /codon_start=1 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA" CDS 1401..5030 /codon_start=1 /label=EGFR /note="precursor of human epidermal growth factor receptor" /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" promoter 5075..5404 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 5414..6010 /codon_start=1 /label=PuroR /note="puromycin N-acetyltransferase" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" LTR 6112..6703 /label=LTR /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(6833..6850) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7004..7592) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7755..8612) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(8613..8717) /label=AmpR promoter ORIGIN 1 cccgaaaagt gccacctgca gcctgaatat gggccaaaca ggatatctgt ggtaagcagt 61 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata 121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg 181 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg 241 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc 301 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct 361 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc 421 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc 481 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga 541 cccaccaccg ggaggcaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt 601 ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc 661 ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca 721 gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga 781 ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt 841 ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca 901 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc 961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg 1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat 1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc 1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca 1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc 1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt 1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggccgga 1381 tcccagtgtg gtggtacatc atgcgaccct ccgggacggc cggggcagcg ctcctggcgc 1441 tgctggctgc gctctgcccg gcgagtcggg ctctggagga aaagaaagtt tgccaaggca 1501 cgagtaacaa gctcacgcag ttgggcactt ttgaagatca ttttctcagc ctccagagga 1561 tgttcaataa ctgtgaggtg gtccttggga atttggaaat tacctatgtg cagaggaatt 1621 atgatctttc cttcttaaag accatccagg aggtggctgg ttatgtcctc attgccctca 1681 acacagtgga gcgaattcct ttggaaaacc tgcagatcat cagaggaaat atgtactacg 1741 aaaattccta tgccttagca gtcttatcta actatgatgc aaataaaacc ggactgaagg 1801 agctgcccat gagaaattta caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc 1861 ctgccctgtg caacgtggag agcatccagt ggcgggacat agtcagcagt gactttctca 1921 gcaacatgtc gatggacttc cagaaccacc tgggcagctg ccaaaagtgt gatccaagct 1981 gtcccaatgg gagctgctgg ggtgcaggag aggagaactg ccagaaactg accaaaatca 2041 tctgtgccca gcagtgctcc gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca 2101 accagtgtgc tgcaggctgc acaggccccc gggagagcga ctgcctggtc tgccgcaaat 2161 tccgagacga agccacgtgc aaggacacct gccccccact catgctctac aaccccacca 2221 cgtaccagat ggatgtgaac cccgagggca aatacagctt tggtgccacc tgcgtgaaga 2281 agtgtccccg taattatgtg gtgacagatc acggctcgtg cgtccgagcc tgtggggccg 2341 acagctatga gatggaggaa gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc 2401 gcaaagtgtg taacggaata ggtattggtg aatttaaaga ctcactctcc ataaatgcta 2461 cgaatattaa acacttcaaa aactgcacct ccatcagtgg cgatctccac atcctgccgg 2521 tggcatttag gggtgactcc ttcacacata ctcctcctct ggatccacag gaactggata 2581 ttctgaaaac cgtaaaggaa atcacagggt ttttgctgat tcaggcttgg cctgaaaaca 2641 ggacggacct ccatgccttt gagaacctag aaatcatacg cggcaggacc aagcaacatg 2701 gtcagttttc tcttgcagtc gtcagcctga acataacatc cttgggatta cgctccctca 2761 aggagataag tgatggagat gtgataattt caggaaacaa aaatttgtgc tatgcaaata 2821 caataaactg gaaaaaactg tttgggacct ccggtcagaa aaccaaaatt ataagcaaca 2881 gaggtgaaaa cagctgcaag gccacaggcc aggtctgcca tgccttgtgc tcccccgagg 2941 gctgctgggg cccggagccc agggactgcg tctcttgccg gaatgtcagc cgaggcaggg 3001 aatgcgtgga caagtgcaac cttctggagg gtgagccaag ggagtttgtg gagaactctg 3061 agtgcataca gtgccaccca gagtgcctgc ctcaggccat gaacatcacc tgcacaggac 3121 ggggaccaga caactgtatc cagtgtgccc actacattga cggcccccac tgcgtcaaga 3181 cctgcccggc aggagtcatg ggagaaaaca acaccctggt ctggaagtac gcagacgccg 3241 gccatgtgtg ccacctgtgc catccaaact gcacctacgg atgcactggg ccaggtcttg 3301 aaggctgtcc aacgaatggg cctaagatcc cgtccatcgc cactgggatg gtgggggccc 3361 tcctcttgct gctggtggtg gccctgggga tcggcctctt catgcgaagg cgccacatcg 3421 ttcggaagcg cacgctgcgg aggctgctgc aggagaggga gcttgtggag cctcttacac 3481 ccagtggaga agctcccaac caagctctct tgaggatctt gaaggaaact gaattcaaaa 3541 agatcaaagt gctgggctcc ggtgcgttcg gcacggtgta taagggactc tggatcccag 3601 aaggtgagaa agttaaaatt cccgtcgcta tcaaggaatt aagagaagca acatctccga 3661 aagccaacaa ggaaatcctc gatgaagcct acgtgatggc cagcgtggac aacccccacg 3721 tgtgccgcct gctgggcatc tgcctcacct ccaccgtgca actcatcacg cagctcatgc 3781 ccttcggctg cctcctggac tatgtccggg aacacaaaga caatattggc tcccagtacc 3841 tgctcaactg gtgtgtgcag atcgcaaagg gcatgaacta cttggaggac cgtcgcttgg 3901 tgcaccgcga cctggcagcc aggaacgtac tggtgaaaac accgcagcat gtcaagatca 3961 cagattttgg gctggccaaa ctgctgggtg cggaagagaa agaataccat gcagaaggag 4021 gcaaagtgcc tatcaagtgg atggcattgg aatcaatttt acacagaatc tatacccacc 4081 agagtgatgt ctggagctac ggggtgaccg tttgggagtt gatgaccttt ggatccaagc 4141 catatgacgg aatccctgcc agcgagatct cctccatcct ggagaaagga gaacgcctcc 4201 ctcagccacc catatgtacc atcgatgtct acatgatcat ggtcaagtgc tggatgatag 4261 acgcagatag tcgcccaaag ttccgtgagt tgatcatcga attctccaaa atggcccgag 4321 acccccagcg ctaccttgtc attcaggggg atgaaagaat gcatttgcca agtcctacag 4381 actccaactt ctaccgtgcc ctgatggatg aagaagacat ggacgacgtg gtggatgccg 4441 acgagtacct catcccacag cagggcttct tcagcagccc ctccacgtca cggactcccc 4501 tcctgagctc tctgagtgca accagcaaca attccaccgt ggcttgcatt gatagaaatg 4561 ggctgcaaag ctgtcccatc aaggaagaca gcttcttgca gcgatacagc tcagacccca 4621 caggcgcctt gactgaggac agcatagacg acaccttcct cccagtgcct gaatacataa 4681 accagtccgt tcccaaaagg cccgctggct ctgtgcagaa tcctgtctat cacaatcagc 4741 ctctgaaccc cgcgcccagc agagacccac actaccagga cccccacagc actgcagtgg 4801 gcaaccccga gtatctcaac actgtccagc ccacctgtgt caacagcaca ttcgacagcc 4861 ctgcccactg ggcccagaaa ggcagccacc aaattagcct ggacaaccct gactaccagc 4921 aggacttctt tcccaaggaa gccaagccaa atggcatctt taagggctcc acagctgaaa 4981 atgcagaata cctaagggtc gcgccacaaa gcagtgaatt tattggagca tgagatgtag 5041 gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc 5101 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 5161 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 5221 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 5281 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 5341 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 5401 caaaaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 5461 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 5521 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 5581 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 5641 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 5701 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 5761 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 5821 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 5881 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 5941 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 6001 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc 6061 ccatgcatcg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga 6121 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa 6181 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata 6241 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga 6301 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc 6361 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca 6421 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac 6481 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag 6541 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt 6601 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg 6661 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa 6721 aattaatttg gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa 6781 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 6841 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 6901 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 6961 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 7021 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 7081 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 7141 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 7201 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 7261 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 7321 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 7381 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 7441 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 7501 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 7561 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 7621 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 7681 ggatcttcac ctagatcctt ttgcggccgc aaatcaatct aaagtatata tgagtaaact 7741 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 7801 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 7861 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 7921 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 7981 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 8041 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 8101 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 8161 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 8221 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 8281 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 8341 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 8401 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 8461 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 8521 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 8581 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 8641 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 8701 caaatagggg ttccgcgcac atttc //