LOCUS       62056_646       11420 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        328..735
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             785..4885
                     /codon_start=1
                     /label=dCas9
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEEKEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4913..5062
                     /codon_start=1
                     /label=VP64
                     /note="tetrameric repeat of the minimal activation domain
                     of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5087..5107
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             5537..5893
                     /codon_start=1
                     /label=RelA (p65) AD
                     /note="transcriptional activation domain of human RelA,
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5912..6481
                     /codon_start=1
                     /label=Rta AD
                     /note="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     misc_RNA        7043..7118
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gene            7285..8640
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     rep_origin      complement(8645..9627)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(9688..10500)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      10725..11313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccctgaattc gcatctagac tgaactggcc gataattgca gacgaacgcc ttgccaacag
       61 ggagttcttc agagacatgg aggctcaaaa cgaaattatt gacagcctag acatcaatag
      121 tcatacaaca gaaagcgacc acccaacttt ggctgataat agcgtataaa caatgcatac
      181 tttgtacgtt caaaatacaa tgcagtagat atatttatgc atattacata taatacatat
      241 cacataggaa gcaacaggcg cgttggactt ttaattttcg aggaccgcga atccttacat
      301 cacacccaat cccccacaag tgatccccca cacaccatag cttcaaaatg tttctactcc
      361 ttttttactc ttccagattt tctcggactc cgcgcatcgc cgtaccactt caaaacaccc
      421 aagcacagca tactaaattt cccctctttc ttcctctagg gtgtcgttaa ttacccgtac
      481 taaaggtttg gaaaagaaaa aagacaccgc ctcgtttctt tttcttcgtc gaaaaaggca
      541 ataaaaattt ttatcacgtt tctttttctt gaaaattttt ttttttgatt tttttctctt
      601 tcgatgacct cccattgata tttaagttaa taaacggtca tcaatttctc aagtttcagt
      661 ttcatttttc ttgttctatt acaacttttt ttacttcttg ctcattagaa agaaagcata
      721 gcaatctaat ctaagtttta attacaaaag atctatgcca aagaagaaga gaaaggtagg
      781 cagcgacaag aagtattcta tcggactggc catcgggact aatagcgtcg ggtgggccgt
      841 gatcactgac gagtacaagg tgccctctaa gaagttcaag gtgctcggga acaccgaccg
      901 gcattccatc aagaaaaatc tgatcggagc tctcctcttt gattcagggg aaaccgctga
      961 agcaacccgc ctcaagcgga ctgctagacg gcggtacacc aggaggaaga accggatttg
     1021 ttaccttcaa gagatattct ccaacgaaat ggcaaaggtc gacgacagct tcttccatag
     1081 gctggaagaa tcattcctcg tggaagagga taagaagcat gaacggcatc ccatcttcgg
     1141 taatatcgtc gacgaggtgg cctatcacga gaaataccca accatctacc atcttcgcaa
     1201 aaagctggtg gactcaaccg acaaggcaga cctccggctt atctacctgg ccctggccca
     1261 catgatcaag ttcagaggcc acttcctgat cgagggcgac ctcaatcctg acaatagcga
     1321 tgtggataaa ctgttcatcc agctggtgca gacttacaac cagctctttg aagagaaccc
     1381 catcaatgca agcggagtcg atgccaaggc cattctgtca gcccggctgt caaagagccg
     1441 cagacttgag aatcttatcg ctcagctgcc gggtgaaaag aaaaatggac tgttcgggaa
     1501 cctgattgct ctttcacttg ggctgactcc caatttcaag tctaatttcg acctggcaga
     1561 ggatgccaag ctgcaactgt ccaaggacac ctatgatgac gatctcgaca acctcctggc
     1621 ccagatcggt gaccaatacg ccgacctttt ccttgctgct aagaatcttt ctgacgccat
     1681 cctgctgtct gacattctcc gcgtgaacac tgaaatcacc aaggcccctc tttcagcttc
     1741 aatgattaag cggtatgatg agcaccacca ggacctgacc ctgcttaagg cactcgtccg
     1801 gcagcagctt ccggagaagt acaaggaaat cttctttgac cagtcaaaga atggatacgc
     1861 cggctacatc gacggaggtg cctcccaaga ggaattttat aagtttatca aacctatcct
     1921 tgagaagatg gacggcaccg aagagctcct cgtgaaactg aatcgggagg atctgctgcg
     1981 gaagcagcgc actttcgaca atgggagcat tccccaccag atccatcttg gggagcttca
     2041 cgccatcctt cggcgccaag aggacttcta cccctttctt aaggacaaca gggagaagat
     2101 tgagaaaatt ctcactttcc gcatccccta ctacgtggga cccctcgcca gaggaaatag
     2161 ccggtttgct tggatgacca gaaagtcaga agaaactatc actccctgga acttcgaaga
     2221 ggtggtggac aagggagcca gcgctcagtc attcatcgaa cggatgacta acttcgataa
     2281 gaacctcccc aatgagaagg tcctgccgaa acattccctg ctctacgagt actttaccgt
     2341 gtacaacgag ctgaccaagg tgaaatatgt caccgaaggg atgaggaagc ccgcattcct
     2401 gtcaggcgaa caaaagaagg caattgtgga ccttctgttc aagaccaata gaaaggtgac
     2461 cgtgaagcag ctgaaggagg actatttcaa gaaaattgaa tgcttcgact ctgtggagat
     2521 tagcggggtc gaagatcggt tcaacgcaag cctgggtacc taccatgatc tgcttaagat
     2581 catcaaggac aaggattttc tggacaatga ggagaaagag gacatccttg aggacattgt
     2641 cctgactctc actctgttcg aggaccggga aatgatcgag gagaggctta agacctacgc
     2701 ccatctgttc gacgataaag tgatgaagca acttaaacgg agaagatata ccggatgggg
     2761 acgccttagc cgcaaactca tcaacggaat ccgggacaaa cagagcggaa agaccattct
     2821 tgatttcctt aagagcgacg gattcgctaa tcgcaacttc atgcaactta tccatgatga
     2881 ttccctgacc tttaaggagg acatccagaa ggcccaagtg tctggacaag gtgactcact
     2941 gcacgagcat atcgcaaatc tggctggttc acccgctatt aagaagggta ttctccagac
     3001 cgtgaaagtc gtggacgagc tggtcaaggt gatgggtcgc cataaaccag agaacattgt
     3061 catcgagatg gccagggaaa accagactac ccagaaggga cagaagaaca gcagggagcg
     3121 gatgaaaaga attgaggaag ggattaagga gctcgggtca cagatcctta aagagcaccc
     3181 ggtggaaaac acccagcttc agaatgagaa gctctatctg tactaccttc aaaatggacg
     3241 cgatatgtat gtggaccaag agcttgatat caacaggctc tcagactacg acgtggacgc
     3301 catcgtccct cagagcttcc tcaaagacga ctcaattgac aataaggtgc tgactcgctc
     3361 agacaagaac cggggaaagt cagataacgt gccctcagag gaagtcgtga aaaagatgaa
     3421 gaactattgg cgccagcttc tgaacgcaaa gctgatcact cagcggaagt tcgacaatct
     3481 cactaaggct gagaggggcg gactgagcga actggacaaa gcaggattca ttaaacggca
     3541 acttgtggag actcggcaga ttactaaaca tgtagcccaa atccttgact cacgcatgaa
     3601 taccaagtac gacgaaaacg acaaacttat ccgcgaggtg aaggtgatta ccctgaagtc
     3661 caagctggtc agcgatttca gaaaggactt tcaattctac aaagtgcggg agatcaataa
     3721 ctatcatcat gctcatgacg catatctgaa tgccgtggtg ggaaccgccc tgatcaagaa
     3781 gtacccaaag ctggaaagcg agttcgtgta cggagactac aaggtctacg acgtgcgcaa
     3841 gatgattgcc aaatctgagc aggagatcgg aaaggccacc gcaaagtact tcttctacag
     3901 caacatcatg aatttcttca agaccgaaat cacccttgca aacggtgaga tccggaagag
     3961 gccgctcatc gagactaatg gggagactgg cgaaatcgtg tgggacaagg gcagagattt
     4021 cgctaccgtg cgcaaagtgc tttctatgcc tcaagtgaac atcgtgaaga aaaccgaggt
     4081 gcaaaccgga ggcttttcta aggaatcaat cctccccaag cgcaactccg acaagctcat
     4141 tgcaaggaag aaggattggg accctaagaa gtacggcgga ttcgattcac caactgtggc
     4201 ttattctgtc ctggtcgtgg ctaaggtgga aaaaggaaag tctaagaagc tcaagagcgt
     4261 gaaggaactg ctgggtatca ccattatgga gcgcagctcc ttcgagaaga acccaattga
     4321 ctttctcgaa gccaaaggtt acaaggaagt caagaaggac cttatcatca agctcccaaa
     4381 gtatagcctg ttcgaactgg agaatgggcg gaagcggatg ctcgcctccg ctggcgaact
     4441 tcagaagggt aatgagctgg ctctcccctc caagtacgtg aatttcctct accttgcaag
     4501 ccattacgag aagctgaagg ggagccccga ggacaacgag caaaagcaac tgtttgtgga
     4561 gcagcataag cattatctgg acgagatcat tgagcagatt tccgagtttt ctaaacgcgt
     4621 cattctcgct gatgccaacc tcgataaagt ccttagcgca tacaataagc acagagacaa
     4681 accaattcgg gagcaggctg agaatatcat ccacctgttc accctcacca atcttggtgc
     4741 ccctgccgca ttcaagtact tcgacaccac catcgaccgg aaacgctata cctccaccaa
     4801 agaagtgctg gacgccaccc tcatccacca gagcatcacc ggactttacg aaactcggat
     4861 tgacctctca cagctcggag gggatcccaa gaaaaagcgc aaggtaggat ccgacgcatt
     4921 ggacgatttt gatctggata tgctgggaag tgacgccctc gatgattttg accttgacat
     4981 gcttggttcg gatgcccttg atgactttga cctcgacatg ctcggcagtg acgcccttga
     5041 tgatttcgac ctggacatgc tgattaactc tagaagttcc ggatctccga aaaagaaacg
     5101 caaagttggt agccagtacc tgcccgacac cgacgaccgg caccggatcg aggaaaagcg
     5161 gaagcggacc tacgagacat tcaagagcat catgaagaag tcccccttca gcggccccac
     5221 cgaccctaga cctccaccta gaagaatcgc cgtgcccagc agatccagcg ccagcgtgcc
     5281 aaaacctgcc ccccagcctt accccttcac cagcagcctg agcaccatca actacgacga
     5341 gttccctacc atggtgttcc ccagcggcca gatctctcag gcctctgctc tggctccagc
     5401 ccctcctcag gtgctgcctc aggctcctgc tcctgcacca gctccagcca tggtgtctgc
     5461 actggctcag gcaccagcac ccgtgcctgt gctggctcct ggacctccac aggctgtggc
     5521 tccaccagcc cctaaaccta cacaggccgg cgagggcaca ctgtctgaag ctctgctgca
     5581 gctgcagttc gacgacgagg atctgggagc cctgctggga aacagcaccg atcctgccgt
     5641 gttcaccgac ctggccagcg tggacaacag cgagttccag cagctgctga accagggcat
     5701 ccctgtggcc cctcacacca ccgagcccat gctgatggaa taccccgagg ccatcacccg
     5761 gctcgtgaca ggcgctcaga ggcctcctga tccagctcct gcccctctgg gagcaccagg
     5821 cctgcctaat ggactgctgt ctggcgacga ggacttcagc tctatcgccg acatggactt
     5881 ctccgcactg ctgggtagcg gatcgggatc tcgggattcc agggaaggga tgtttttgcc
     5941 gaagcctgag gccggctccg ctattagtga cgtgtttgag ggccgcgagg tgtgccagcc
     6001 aaaacgaatc cggccatttc atcctccagg aagtccatgg gccaaccgcc cactccccgc
     6061 cagcctcgca ccaacaccaa ccggtccagt acatgagcca gtcgggtcac tgaccccggc
     6121 accagtccct cagccactgg atccagcgcc cgcagtgact cccgaggcca gtcacctgtt
     6181 ggaggatccc gatgaagaaa cgagccaggc tgtcaaagcc cttcgggaga tggccgatac
     6241 tgtgattccc cagaaggaag aggctgcaat ctgtggccaa atggaccttt cccatccgcc
     6301 cccaaggggc catctggatg agctgacaac cacacttgag tccatgaccg aggatctgaa
     6361 cctggactca cccctgaccc cggaattgaa cgagattctg gataccttcc tgaacgacga
     6421 gtgcctcttg catgccatgc atatcagcac aggactgtcc atcttcgaca catctctgtt
     6481 ttaactcgag tggcataaag caatcttgat gaggataatg attttttttt gaatatacat
     6541 aaatactacc gtttttctgc tagattttgt gatgacgtaa ataagtacat attacttttt
     6601 aagccaagac aagattaagc attaacttta cccttttctt tctaagtttc aatattagtt
     6661 atcactgttt aaaagttatg gcgagaacgt cggcggttaa aatatattac cctgaacggc
     6721 tgccaaacca gatgtcaaca cagctacaac gatgtgcttc agtattacat tttttgcctt
     6781 caacgccttg attgttctat ttttgctaat aataaatcta tttcatcgga ctaaaagtcc
     6841 attagttgta agcggattta gctcagttgg gagagcgcca gactgaagaa aaacttcggt
     6901 caagtcatct ggaggtcctg tgttcgatcc acagaattcg cagatggccg gcatggtccc
     6961 agcctcctcg ctggcgccgg ctgggcaaca ccttcgggtg gcgaatggga ctcgctatcg
     7021 tcttctcacg aagacttact cagttttaga gctagaaata gcaagttaaa ataaggctag
     7081 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttatttt ttgtcactat
     7141 tgttatgtaa aatgccacct ctgacagtat ggaacgcaaa cttctgtcta gtggatagct
     7201 gagcagttac agagatgtta cgaaccacta gtgcactgca gtacagttta gcttgcctcg
     7261 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
     7321 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
     7381 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
     7441 ggctcctcgc tccagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
     7501 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
     7561 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
     7621 aaacaaaaat gggtaaggaa aagactcacg tttcgaggcc gcgattaaat tccaacatgg
     7681 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     7741 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     7801 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     7861 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     7921 cgatccccgg caaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     7981 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     8041 cttttaacag cgatcgcgta tttcgtctgg ctcaggcgca atcacgaatg aataacggtt
     8101 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     8161 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     8221 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     8281 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     8341 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     8401 aattgcagtt tcatttgatg ctcgatgagt ttttctaaag taactgacaa taaaaagatt
     8461 cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa
     8521 tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag
     8581 ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg
     8641 gagtaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
     8701 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
     8761 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
     8821 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
     8881 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
     8941 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
     9001 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
     9061 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
     9121 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
     9181 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
     9241 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
     9301 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
     9361 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
     9421 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
     9481 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
     9541 agtccggtgc gtttttggtt ttttgaaagt gcgtcatcag agcgcttttg gttttcaaaa
     9601 gcgctctgaa gttcctatac tttctagcta gagaatagga acttcccgag cggccgcgtg
     9661 ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa
     9721 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     9781 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     9841 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     9901 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc
     9961 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
    10021 caaaccgtta ttcattcgtg attgcgcctg agcgaggcga aatacgcgat cgctgttaaa
    10081 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
    10141 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
    10201 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
    10261 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
    10321 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
    10381 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
    10441 atccatgttg gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa tatggctcat
    10501 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
    10561 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa
    10621 tcgaactttt gctgagttga aggatcagtc atgaccaaaa tcccttaacg tgagttttcg
    10681 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10741 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10801 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10861 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10921 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10981 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    11041 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    11101 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    11161 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    11221 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    11281 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11341 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    11401 gtggataacc gtgcggccgc
//
