LOCUS Exported 9030 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Enterobacteria phage M13 vector DY3F63, complete sequence. ACCESSION AY754023 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9030) AUTHORS Hoet RM, Coehn EH, Kent RB, Rookey K, Schoonbroodt S, Hogan S, Rem L, Frans N, Daukandt M, Pieters H, van Hegelsom R, Coolen-van Neer N, Nastri HG, Rondon IJ, Leeds J, Hufton SE, Huang L, Kashin I, Devlin M, Kuang G, Steukers M, Viswanathan M, Nixon AE, Sexton DJ, Hoogenboom HR, Ladner RC. TITLE Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity JOURNAL Nat. Biotechnol. 23 (3), 344-348 (2005) PUBMED 15723048 REFERENCE 2 (bases 1 to 9030) AUTHORS Ladner RC, Hoogenboom HR, Hoet RM, Cohen EH, Kashin I, Rondon IJ, Rem L, Frans N, Schoonbroodt S, Kent RB, Rookey K, Hogan S. TITLE Direct Submission JOURNAL Submitted (13-SEP-2004) Research, Dyax Corp, 300 Technology Square, Cambridge, MA 02139, USA REFERENCE 3 (bases 1 to 9030) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..9030 /organism="Enterobacteria phage M13 vector DY3F63" /mol_type="other DNA" /note="derived from M13mp18 phage cloning vector in GenBank Accession Number M77815; has high-affinity synthetic and donor-derived diversity" /db_xref="taxon:296376" CDS 843..1106 /codon_start=1 /gene="V" /product="pV" /label=M13 gene V /note="replication" /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK" CDS 1108..1209 /codon_start=1 /gene="VII" /product="pVII" /label=M13 gene VII /note="minor coat protein" /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR" CDS 1206..1304 /codon_start=1 /gene="IX" /product="pIX" /label=M13 gene IX /note="minor coat protein" /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS" CDS 1301..1522 /codon_start=1 /gene="VIII" /product="pVIII" /label=M13 gene VIII /note="major coat protein" /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEYI GYAWAMVVVIVGATIGIKLFKKFTSKAS" terminator 1526..1574 /label=fd terminator /note="central terminator from bacteriophage fd (Otsuka and Kunisawa, 1982)" CDS 1579..2853 /codon_start=1 /gene="III" /product="pIII" /label=M13 gene III /note="minor coat protein" /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKSHTENSFTNVWKDDKTL DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDLFVCEYQ GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF AFLLYVATFMYVFSTFANILRNKES" CDS 2856..3194 /codon_start=1 /gene="VI" /product="pVI" /label=M13 gene VI /note="minor coat protein" /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI VSYLDWDK" CDS 3196..4242 /codon_start=1 /gene="I" /product="pI" /label=M13 gene I /note="phage assembly" /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN SNEIVKCN" CDS 4220..5500 /codon_start=1 /gene="IV" /product="pIV" /label=M13 gene IV /note="phage assembly" /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG LLFSSRSDSNEESTLYVLVKATIVRAL" rep_origin 5488..5868 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6040..6144 /gene="bla" /label=AmpR promoter CDS 6145..7005 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=bla /note="ApR" /protein_id="AAV54522.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGALVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene 6145..7005 /gene="bla" /label=bla protein_bind 7264..7285 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7300..7330 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7338..7354 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7362..7378 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" misc_feature 7425..7481 /note="encodes light chain signal sequence; antibody stuffer" misc_feature 7491..7536 /label=encodes light chain antibody stuffer /note="encodes light chain antibody stuffer" misc_feature 7563..7628 /note="encodes heavy chain signal sequence; antibody stuffer" misc_feature 7632..7669 /label=encodes heavy chain antibody stuffer /note="encodes heavy chain antibody stuffer" misc_feature 7692..7709 /note="encodes hexahistidine tag; hexa-His tag" CDS 7692..7709 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 7719..7748 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" misc_feature 7776..8147 /note="encodes domain 3 of protein III; antibody stuffer" misc_feature 8148..8216 /note="encodes transmembrane segment; antibody stuffer" CDS 8629..831 /codon_start=1 /gene="II" /product="pII" /label=M13 gene II /note="replication" /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGINLSAR TVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL RNVSNGQTKSTRSQNWESTVIWNETSRHRTLVAYLKHVELQHYIQQLSSKPSAKMTSYQ KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP DWYNEPVLKIA" ORIGIN 1 aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat 61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact 121 cgttcgcaga attgggaatc aactgttata tggaatgaaa cttccagaca ccgtacttta 181 gttgcatatt taaaacatgt tgagctacag cattatattc agcaattaag ctctaagcca 241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg 301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag 361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt 421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca 481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct 541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt 601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt 661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg 721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt 781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca 841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt 901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg 961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc 1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc 1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat 1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt 1201 caaagatgag tgttttagtg tattcttttg cctctttcgt tttaggttgg tgccttcgta 1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct 1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga 1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta 1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa 1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt 1561 tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc 1621 tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aatcccatac agaaaattca 1681 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt 1741 ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca 1801 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt 1861 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct 1921 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa 1981 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt 2041 cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact 2101 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg 2161 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag 2221 gatttatttg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat 2281 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt 2341 ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt 2401 gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat 2461 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt 2521 gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact 2581 ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct 2641 ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct 2701 tttgtctttg gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta 2761 ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg 2821 tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt 2881 tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc 2941 ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg 3001 ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact 3061 ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc 3121 tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg 3181 attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg 3241 ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat 3301 cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt 3361 cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat 3421 tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat 3481 acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt 3541 aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg 3601 cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct 3661 tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat 3721 gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat 3781 actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat 3841 tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta 3901 aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt 3961 tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg 4021 gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct 4081 cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat 4141 agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc 4201 attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt 4261 tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt 4321 tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg 4381 tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc 4441 tgttttacgt gcaaataatt ttgatatggt aggttctaac ccttccataa ttcagaagta 4501 taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga 4561 tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac 4621 ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa 4681 gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt 4741 tagtgctcct aaagatattt tagataacct tcctcaattc ctttcaactg ttgatttgcc 4801 aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga 4861 tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg 4921 cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt 4981 agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg 5041 tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat 5101 tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg 5161 tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt 5221 tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat 5281 tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact 5341 cggtggcctc actgattata aaaacacttc tcaggattct ggcgtaccgt tcctgtctaa 5401 aatcccttta atcggcctcc tgtttagctc ccgctctgat tctaacgagg aaagcacgtt 5461 atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg 5521 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 5581 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 5641 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 5701 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 5761 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 5821 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa 5881 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg 5941 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct 6001 ggatccaagc ttgcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 6061 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 6121 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 6181 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 6241 gatgctgaag atcagttggg cgcactagtg ggttacatcg aactggatct caacagcggt 6301 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 6361 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 6421 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 6481 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 6541 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 6601 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 6661 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 6721 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 6781 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 6841 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 6901 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 6961 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 7021 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 7081 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactgt 7141 acgtaagacc cccaagcttg tcgactgaat ggcgaatggc gctttgcctg gtttccggca 7201 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgacgctcg agcgcaacgc 7261 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 7321 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 7381 tgattacgcc aagctttgga gccttttttt tggagatttt caacgtgaaa aaattattat 7441 tcgcaattcc tttagttgtt cctttctatt ctcacagtgc acagtgatag actagttaga 7501 cgcgtgctta aaggcctcca atcctcttgg cgcgccaatt ctatttcaag gagacagtca 7561 taatgaaata cctattgcct acggcagccg ctggattgtt attactcgcg gcccagccgg 7621 ccctctgata agatatcact tgtttaaact ctgcttggcc ctcttggcct tctagtagac 7681 ttgcggccgc acatcatcat caccatcacg gggccgcaga acaaaaactc atctcagaag 7741 aggatctgaa tggggccgca gaggctagct ctgctagtgg cgacttcgac tacgagaaaa 7801 tggctaatgc caacaaaggc gccatgactg agaacgctga cgagaatgct ttgcaaagcg 7861 atgccaaggg taagttagac agcgtcgcga ccgactatgg cgccgccatc gacggcttta 7921 tcggcgatgt cagtggtttg gccaacggca acggagccac cggagacttc gcaggttcga 7981 attctcagat ggcccaggtt ggagatgggg acaacagtcc gcttatgaac aactttagac 8041 agtaccttcc gtctcttccg cagagtgtcg agtgccgtcc attcgttttc ggtgccggca 8101 agccttacga gttcagcatc gactgcgata agatcaatct tttccgcggc gttttcgctt 8161 tcttgctata cgtcgctact ttcatgtacg ttttcagcac tttcgccaat attttacgca 8221 acaaagaaag ctagtgatct cctaggaagc ccgcctaatg agcgggcttt ttttttctgg 8281 tatgcatcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga 8341 tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac 8401 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga 8461 aggccagacg cgaattattt ttgatggcgt tcctattggt taaaaaatga gctgatttaa 8521 caaaaattta atgcgaattt taacaaaata ttaacgttta caatttaaat atttgcttat 8581 acaatcttcc tgtttttggg gcttttctga ttatcaaccg gggtacatat gattgacatg 8641 ctagttttac gattaccgtt catcgattct cttgtttgct ccagactctc aggcaatgac 8701 ctgatagcct ttgtagatct ctcaaaaata gctaccctct ccggcattaa tttatcagct 8761 agaacggttg aatatcatat tgatggtgat ttgactgtct ccggcctttc tcaccctttt 8821 gaatctttac ctacacatta ctcaggcatt gcatttaaaa tatatgaggg ttctaaaaat 8881 ttttatcctt gcgttgaaat aaaggcttct cccgcaaaag tattacaggg tcataatgtt 8941 tttggtacaa ccgatttagc tttatgctct gaggctttat tgcttaattt tgctaattct 9001 ttgccttgcc tgtatgattt attggatgtt //