LOCUS       V016808                 8575 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016808
VERSION     V016808
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             957..3974
                     /gene="OPG071"
                     /label="DNA polymerase"
                     /note="DNA polymerase from Vaccinia virus (strain Western
                     Reserve). Accession#: P06856"
     CDS             4072..4113
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             4123..4140
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    4169..4393
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4439..4867
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4881..5211
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5278..6069
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6248..6381
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6418..6434)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6442..6458)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6466..6496)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6511..6532)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6820..7408)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7582..8439)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8440..8544)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccga attccagctg accaccatgg
      961 atgttcggtg cattaattgg tttgaaagtc acggtgaaaa cagattttta tatctgaaat
     1021 ccagatgtcg aaatggtgag accgtattta tacgatttcc tcattacttt tattacgtag
     1081 tgacggacga aatatatcag tcattgtctc ctcctccatt taatgcgagg ccgttgggaa
     1141 agatgagaac tatagacatt gacgagacaa taagttataa tctagatatt aaagatagaa
     1201 aatgctccgt cgcagatatg tggttgatag aagagccaaa gaaacgcagc atacaaaatg
     1261 ccaccatgga tgaatttctc aatattagtt ggttttatat ttctaacggg atatctccag
     1321 acggatgtta ctcgttggac gagcaatatt tgacaaagat taacaatgga tgttatcatt
     1381 gtgacgatcc acgtaactgt ttcgctaaaa aaatacctag attcgatatc ccaagatcgt
     1441 acttatttct agatatagag tgtcacttcg ataagaagtt tccttctgta tttattaacc
     1501 caatctcgca tacaagttac tgttatatcg atttaagtgg taaacgatta ttgtttacgc
     1561 tcattaatga agagatgtta acggaacagg aaatacaaga agccgtcgat agaggatgtt
     1621 tgaggataca gtcactaatg gaaatggatt acgaacgaga actagtttta tgttctgaaa
     1681 tagttttgtt acgaatagct aaacaattgt tggaactaac gttcgactat gtcgttacct
     1741 ttaacggaca taactttgat ctgagatata ttactaatcg tctagagtta ttaacaggag
     1801 agaagattat ctttagatct ccggacaaaa aggaagctgt atatctctgt atttatgaga
     1861 gaaatcagtc tagtcataag ggagtaggcg gcatggccaa tactacgttt cacgttaata
     1921 acaataatgg aactatattt ttcgatctat attcattcat tcaaaaatct gaaaaattgg
     1981 attcgtacaa attggattct atatccaaga acgcgttcag ttgcatgggt aaagtattaa
     2041 atagaggagt tagagaaatg acgttcatcg gtgacgatac tacggacgcg aaaggcaaag
     2101 ccgctgcatt tgcaaaggtt ttaaccacag gtaattatgt gactgttgat gaggatatta
     2161 tatgtaaagt aattcgtaaa gatatttggg aaaatggatt taaagtcgta ctattatgtc
     2221 ctactttacc taatgataca tataaattat ctttcggaaa ggatgacgtt gatttagctc
     2281 agatgtataa ggattataat ctaaacatag ctttagatat ggctagatac tgtattcatg
     2341 atgcttgttt gtgtcagtat ttgtgggagt attatggagt agaaacaaaa acagacgcgg
     2401 gtgcgtcaac atatgtgctt cctcaatcca tggtattcga atatagagcg agtacagtca
     2461 tcaagggtcc actgttaaag ctattgttgg aaactaaaac tatcttagtt agatcagaaa
     2521 caaaacaaaa gtttccttat gaaggcggta aggtatttgc tccaaaacaa aaaatgttta
     2581 gtaataatgt attaatcttt gattataaca gtctgtatcc taatgtgtgt atctttggaa
     2641 atctatctcc ggaaacatta gtcggtgtcg ttgttagtac caatagattg gaagaagaaa
     2701 taaataatca gctcttgctt cagaaatatc cacctcctag atatattacg gttcattgtg
     2761 aacctagact accgaacctc atctctgaaa tagcaatttt cgatagatcg atagaaggaa
     2821 ccattcctag actattaaga acatttttgg cagagagagc cagatataaa aagatgttaa
     2881 aacaggctac cagttcaact gaaaaggcca tctatgattc catgcaatat acgtacaaga
     2941 tagtagccaa ctcagtatat ggtctgatgg gatttagaaa tagtgctcta tactcatacg
     3001 cttcggctaa gagttgcaca tccataggac gtagaatgat cttgtatcta gaatcggtac
     3061 taaatggagc agagttatct aacggtatgt tacggtttgc caatccatta agtaatccat
     3121 tttatatgga cgatagagat attaatccga ttgtgaaaac atcgttgcct atagattaca
     3181 gatttcgttt tcgtagcgtg tatggagata ccgactccgt gtttacagag atagacagtc
     3241 aagatgtaga taagtccata gaaatagcaa aggagttaga acgactgatt aataatagag
     3301 tattgtttaa taattttaaa atagagtttg aggcggtata taagaatctg attatgcaat
     3361 cgaagaagaa atatacaacg atgaaatact cggcatcgtc gaattcaaaa tctgtacctg
     3421 agagaattaa taaaggtact agtgaaacta gaagagatgt ttccaagttt cataagaata
     3481 tgattaagac atacaagacc agactgtctg agatgttgtc tgaaggacgg atgaattcta
     3541 atcaggtatg tatagatatt ctccgttctt tagaaacaga tttacgatcc gaatttgata
     3601 gtagatcgtc tcctctagaa ttatttatgt tgagtcgaat gcatcactca aattataaat
     3661 ccgcagataa ccctaatatg tatttggtta ctgaatataa taaaaataat ccagaaacta
     3721 tagaacttgg agaacgatat tattttgcat atatttgtcc ggctaatgta ccatggacca
     3781 aaaaacttgt aaatattaaa acatatgaaa caattatcga tagaagtttt aaactcggca
     3841 gtgatcaaag aatattttac gaagtttact ttaaacgatt gacgtccgaa atagtcaatc
     3901 tattggataa taaagtttta tgcatctcat tctttgaaag aatgtttggt tcaaaaccta
     3961 cattttacga agcataagca attcccgggg atccgaataa agggtcaaga caattctgca
     4021 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct
     4081 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat
     4141 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4201 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4261 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4321 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4381 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4561 ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt
     4621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4801 gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4861 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4921 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4981 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5041 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5101 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     5161 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     5221 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     5281 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5341 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5401 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5461 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5521 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5581 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5641 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5701 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5761 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5821 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5881 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5941 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6001 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6061 gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct
     6121 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     6181 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     6241 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6301 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6361 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     6421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6601 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6661 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6721 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6781 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     7021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7261 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7441 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7501 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7561 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7621 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7681 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7741 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7801 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7861 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7921 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7981 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8041 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8101 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8161 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8221 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8281 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8341 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8401 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8461 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8521 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
