LOCUS       40924_565       12828 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary expression vector D3cpv-C, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     FRET-based genetically encoded sensors allow high-resolution live 
            cell imaging of Ca(2) dynamics
  JOURNAL   Plant J. 69 (1), 181-192 (2012)
  PUBMED    21910770
REFERENCE   2  (bases 1 to 12828)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2011) Department of Developmental Biology, Centre 
            for Organismal Studies Heidelberg, Im Neuenheimer Feld 230, 
            Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 12828)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2012"; volume: "69"; issue: "1"; pages: "181-192"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2011) Department of Developmental Biology, Centre for 
            Organismal Studies Heidelberg, Im Neuenheimer Feld 230, Heidelberg 
            69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    303..324
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        339..369
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    377..393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     401..417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      443..1047
                     /label=AtUBQ10
                     /note="AtUBQ10"
                     /regulatory_class="promoter"
     misc_feature    1077..1130
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             1154..1837
                     /label=CFP
                     /note="cyan variant of GFP"
     CDS             2594..3112
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     terminator      3131..3772
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(3785..3801)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(4383..4635)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(4834..5625)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5655..6035)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    complement(6407..6431)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6959..7747
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      7994..8582
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8768..8908)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9252..9446)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9515..10579)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11016..11642)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      241 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      301 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      361 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgactga
      421 ttacgaattc gagctcggta cccgacgagt cagtaataaa cggcgtcaaa gtggttgcag
      481 ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa
      541 ataaggcaat tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt
      601 tattattagc tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt
      661 cttcttcttc ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc
      721 aatttctcaa aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt
      781 ctgtgttcct tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat
      841 atgttctttg gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg
      901 ttagatatca tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata
      961 atttgagttt tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta
     1021 gtttctagtt tgtgcgatcg aatttgtaga ttaatctgag tttttctgat taacagctcg
     1081 agtgcgggat cctctagagg gcccgggacg tccgcggaga tctacgcgtg tcgactcgag
     1141 tgcggccgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1201 ggtcgagctg gacggcgacg taaacggcca caggttcagc gtgtccggcg agggcgaggg
     1261 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1321 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc
     1381 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1441 gcgtaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1501 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1561 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
     1621 caagcagaag aacggcatca aggcccactt caagatccgc cacaacatcg aggacggcag
     1681 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1741 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     1801 cgatcacatg gtcctgctgg agttcgtgac cgccgcccgc atgcatgacc aactgacaga
     1861 agagcagatt gcagagttca aagaagcctt ctcattattg gacaaggatg gggacggcac
     1921 catcaccaca aaggaacttg gcaccgctct gaggtcgctt ggacaaaacc caacggaagc
     1981 agaattgcag gatatgatca atgaagtcga tgctgatggc aatggaacga tttactttcc
     2041 tgaatttctt actatgatgg ctagaaaaat gaaggacaca gacagcgaag aggaaatccg
     2101 agaagcattc cgtgtttttg acaaggatgg gaacggctac atcagcgctg ctgaattacg
     2161 tcacgtcatg acaaacctcg gggagaagtt aacagatgaa gaagttgatg aaatgataag
     2221 ggaagcagat atcgatggtg atggccaagt aaactatgaa gagtttgtac aaatgatgac
     2281 agcaaagggg gggaagaggc gctggcagaa aacaggccat gctgtccgag cattcggaag
     2341 actgaagaag atctccagct ccggggcact ggagctcatg gacggcggcg tgcagctcgc
     2401 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2461 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2521 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
     2581 tggcagcggt ggcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2641 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2701 cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt
     2761 gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc
     2821 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2881 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2941 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3001 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga
     3061 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg agtaaactag
     3121 ttggctgcag agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca
     3181 tcagtttcat tgcgcacaca ccagaatcct actgagttcg agtattatgg cattgggaaa
     3241 catgtttttc ttgtaccatt tgttgtgctt gtaatttact gtgtttttta ttcggttttc
     3301 gctatcgaac tgtgaaatgg aaatggatgg agaagagtta atgaatgata tggtcctttt
     3361 gttcattctc aaattaatat tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa
     3421 aatataagag atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta
     3481 atgaccgaag ttaatatgag gagtaaaaca cttgtagttg taccattatg cttattcact
     3541 aggcaacaaa tatattttca gacctagaaa agctgcaaat gttactgaat acaagtatgt
     3601 cctcttgtgt tttagacatt tatgaacttt cctttatgta attttccaga atccttgtca
     3661 gattctaatc attgctttat aattatagtt atactcatgg atttgtagtt gagtatgaaa
     3721 atatttttta atgcatttta tgacttgcca attgattgac aacatgcatc aatcgaagct
     3781 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     3841 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     3901 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgccaatcg
     3961 aggccgacca accgcaagcg ttgtcagtgt tgcaaagcgc tctgtgtggg cctactttaa
     4021 ttgcttccag tgttaaattg gcgaaaggca ataatatcgc aaaatattgt gttgtaaaat
     4081 gtaattatgt tttaatttca tggaaatgtt tgagcataat ttttattaat gtactaaatt
     4141 actgttttgt taaatgcaat tttgctttct cgggatttta atatcaaaat ctatttagaa
     4201 atacacaata ttttgttgca ggcttgctgg agaatcgatc tgctatcata aaaattacaa
     4261 aaaaatttta tttgcctcaa ttattttagg attggtatta aggacgctta aattatttgt
     4321 cgggtcacta cgcatcattg tgattgagaa gatcagcgat acgaaatatt cgtagtacta
     4381 tcgataattt atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat
     4441 acaaagacag ataaagccac gcacatttag gatattggcc gagattactg aatattgagt
     4501 aagatcacgg aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat
     4561 actcaaaccg ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca
     4621 acatgggagt ccaaggtttc agggaagctg gaattccggg gtgggcgaag aactccagca
     4681 tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca
     4741 acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt
     4801 ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     4861 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     4921 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     4981 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     5041 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc
     5101 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     5161 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     5221 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     5281 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     5341 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     5401 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctggagtt cattcagggc
     5461 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     5521 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     5581 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatccggg
     5641 gaattcgcta gagtcgattt ggtgtatcga gattggttat gaaattcaga tgctagtgta
     5701 atgtattggt aatttgggaa gatataatag gaagcaaggc tatttatcca tttctgaaaa
     5761 ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt cttgctgtaa agcgttgttt
     5821 ggtacacttt tgactagcga ggcttggcgt gtcagcgtat ctattcaaaa gtcgttaatg
     5881 gctgcggatc aagaaaaagt tggaatagaa acagaatacc cgcgaaattc aggcccggtt
     5941 gccatgtcct acacgccgaa ataaacgacc aaattagtag aaaaataaaa actgactcgg
     6001 atacttacgt cacgtcttgc gcactgattt gaaaaatctc aatataaaca aagacggcca
     6061 caagaaaaaa ccaaaacacc gatattcatt aatcttatct agtttctcaa aaaaattcat
     6121 atcttccaca cgtgaaaatg ccaatttctc agacctacct cggctctgcg aaggcccccg
     6181 ctggtatcaa aagtttttat ttcatccgac atggcgcgac cgacctcaac gagaaggaaa
     6241 ttgtcgtgaa cggtgagaag ctctggggcg tgcaaggttc cggaacgaac atcggtctca
     6301 atgcaaaagg ggaacgccag gctctgttgg cccctcgaaa ttcggcgtta attcagtaca
     6361 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     6421 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     6481 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     6541 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     6601 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     6661 ctgtgatcaa ttcgggcacg aacccagtgg acataagcct cgttcggttc gtaagctgta
     6721 atgcaagtag cgtaactgcc gtcacgcaac tggtccagaa ccttgaccga acgcagcggt
     6781 ggtaacggcg cagtggcggt tttcatggct tcttgttatg acatgttttt ttggggtaca
     6841 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     6901 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     6961 gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     7021 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     7081 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     7141 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     7201 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     7261 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     7321 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     7381 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     7441 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     7501 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     7561 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     7621 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     7681 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     7741 cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcc ggcgttaact
     7801 caagcgatta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     7861 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca
     7921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     8161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8761 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     8821 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     8881 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     8941 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     9001 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
     9061 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
     9121 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
     9181 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
     9241 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
     9301 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
     9361 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
     9421 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
     9481 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
     9541 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
     9601 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
     9661 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
     9721 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
     9781 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
     9841 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
     9901 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
     9961 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
    10021 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
    10081 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
    10141 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
    10201 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
    10261 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
    10321 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
    10381 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
    10441 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
    10501 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
    10561 ggccggatgg tttgcgaccg tcacgccgat tcctcgggct tgggggttcc agtgccattg
    10621 cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
    10681 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
    10741 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
    10801 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
    10861 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
    10921 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
    10981 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
    11041 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
    11101 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
    11161 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
    11221 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
    11281 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
    11341 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
    11401 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
    11461 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
    11521 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
    11581 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
    11641 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
    11701 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
    11761 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
    11821 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
    11881 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
    11941 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
    12001 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
    12061 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
    12121 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
    12181 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
    12241 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
    12301 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
    12361 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
    12421 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
    12481 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
    12541 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
    12601 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
    12661 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
    12721 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
    12781 tcagaagccg actgcactat agcagcggag gggttggatc aaagtact
//
