LOCUS       62056_501        3771 bp DNA     circular SYN 03-DEC-2019
DEFINITION  Cloning vector CelR_HTK, complete sequence.
ACCESSION   MN207914
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Rondon RE, Groseclose TM, Short AE, Wilson CJ.
  TITLE     Transcriptional programming using engineered systems of 
            transcription factors and genetic architectures
  JOURNAL   Nat Commun 10 (1), 4784 (2019)
  PUBMED    31636266
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Rondon RE, Groseclose T, Short A, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2019) Chemical and Biomolecular Engineering, 
            Georgia Institute of Technology, 950 Atlantic dr. NW, 5110E, 
            Atlanta, GA 30332, United States
REFERENCE   3  (bases 1 to 3771)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "4784"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-2019) Chemical and Biomolecular Engineering, Georgia 
            Institute of Technology, 950 Atlantic dr. NW, 5110E, Atlanta, GA 
            30332, United States"
FEATURES             Location/Qualifiers
     source          1..3771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        596..673
                     /label=lacI promoter
     CDS             674..1687
                     /codon_start=1
                     /transl_table=11
                     /product="CelR HTK"
                     /label=CelR HTK
                     /protein_id="QFU95493.1"
                     /translation="MKPVTLYDVAEYAGVSHTTVSKVVNQASHVSAKTREKVEAAIKEL
                     GYVPNRAARTLVTRRTDTVALVVSENNQKLFAEPFYAGIVLGVGVALSERGFQFVLATG
                     RSGIEHERLGGYLAGQHVDGVLLLSLHRDDPLPQMLDEAGVPYVYGGRPLGVPEEQVSY
                     VDIDNIGGGRQATQRLIETGHRRIATIAGPQDMVAGVERLQGYREALLAAGMEYDETLV
                     SYGDFTYDSGVAAMRELLDRAPDVDAVFAASDLMGLAALRVLRASGRRVPEDVAVVGYD
                     DSTVAEHAEPPMTSVNQPTELMGREMARLLVDRITGETTEPVRLVLETHLMVRESG"
     protein_bind    1727..1748
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      1939..2483
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        3009..3111
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3112..3768
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
       61 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
      121 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
      181 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga
      241 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggttag
      301 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
      361 atccggaaca taatggtgca gggcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
      421 gccgggggac tgttgggcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
      481 gtcataagtg cggcgacgac accattcctt gcggcggcgg tgctcaacgg cctcaaccta
      541 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac
      601 catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
      661 cagggtggtg aatatgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
      721 tcataccacc gtttccaaag tggtgaacca ggccagccac gtttctgcga aaacgcggga
      781 aaaagtggaa gcggcgatca aagagctggg ctacgtgccg aaccgcgcag cccgcaccct
      841 ggtcacccga cgtaccgaca ccgtagccct ggtggtgtcg gaaaacaacc agaagctctt
      901 cgccgaaccc ttctatgccg ggatcgtgct cggcgtgggg gttgctctgt ccgaacgggg
      961 attccagttc gtcctggcca cgggccgctc cgggatagag catgagcggc tgggcggcta
     1021 cctggccgga cagcacgtcg acggggtcct cctgctgtcg ctccaccgcg acgacccgct
     1081 gccgcagatg ctggacgagg ccggggtgcc gtacgtctac ggcggccgtc cgctcggcgt
     1141 ccccgaagaa caggtgtcct atgtcgatat cgacaacatc ggcgggggac gccaggccac
     1201 ccagcggctg atcgagaccg ggcaccggcg gatcgctacg atcgcgggcc cgcaggacat
     1261 ggtcgctggt gtggaacgcc tccaggggta tcgcgaagca ctgctcgccg cggggatgga
     1321 gtacgacgag acgctggtga gctacggtga cttcacctac gacagcgggg tggccgcgat
     1381 gcgggagctg ctggatcggg cccccgacgt ggacgccgtg ttcgcggcct ccgacttgat
     1441 ggggctggcc gcgctgcggg tgctgcgtgc ttcgggacgc cgcgtgcccg aggatgtggc
     1501 ggtggtcggc tacgacgact cgaccgtagc cgagcacgcc gaaccgccga tgaccagcgt
     1561 caaccagccc accgagctga tgggccggga gatggcccgg ctgctcgtgg accggatcac
     1621 cggggagacc accgaaccgg tgcggctggt gctggagacc catttgatgg tgcgggaatc
     1681 cgggtgatgt acacgagaat tggcttggac tcctgcgcaa cgcaattaat gtaagttagc
     1741 tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct
     1801 ccttccggtg ggcgcggggc atgactaaca tgagaattac aacttatatc gtatggggct
     1861 gacttcaggt gctacatttg aagagataaa ttgcactgaa atctagaaat attttatctg
     1921 attaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa
     1981 cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa
     2041 ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta
     2101 accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg
     2161 gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc
     2221 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
     2281 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta
     2341 aaccgaaagg caggaacagg agagcgcacg agggagccgc caggggaaac gcctggtatc
     2401 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt
     2461 caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag
     2521 tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg
     2581 cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca
     2641 catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg
     2701 acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgatgtccgg
     2761 cggtgctttt gccgttacgc accaccccgt cagtagctga acaggaggga cagctgatag
     2821 aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag taagttggca
     2881 gcatcacccg acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa
     2941 taaataaatc ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa
     3001 tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
     3061 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
     3121 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     3181 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     3241 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     3301 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     3361 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     3421 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     3481 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     3541 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     3601 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     3661 ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga
     3721 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta a
//
