LOCUS       40924_435       10437 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector CasYFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Hahn F, Mantegazza O, Greiner A, Hegemann P, Eisenhut M, Weber AP.
  TITLE     An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis 
            thaliana
  JOURNAL   Front Plant Sci 8, 39 (2017)
  PUBMED    28174584
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Hahn F, Mantegazza O, Eisenhut M, Greiner A, Hegemann P, Weber APM.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2016) Plant Biochemistry, Heinrich Heine 
            University Dusseldorf, Universitatsstr. 1, Dusseldorf 40225, Germany
REFERENCE   3  (bases 1 to 10437)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10437)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci 8, 39 (2017)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2016) Plant Biochemistry, Heinrich Heine University 
            Dusseldorf, Universitatsstr. 1, Dusseldorf 40225, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..362
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(385..401)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(785..860)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      complement(881..1380)
                     /label=U6/C8
                     /note="U6/C8"
                     /regulatory_class="promoter"
     regulatory      1441..2254
                     /label=from Chlamydomonas reinhardtii
                     /note="from Chlamydomonas reinhardtii"
                     /regulatory_class="promoter"
     CDS             2273..2293
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2291..6394
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             6401..7114
                     /label=EYFP
                     /note="enhanced YFP"
     CDS             7130..7186
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7187..7987
                     /codon_start=1
                     /product="aminoglycoside 3'-phosphotransferase VIII"
                     /function="Selection marker in Chlamydomonas reinhardtii"
                     /label=aminoglycoside 3'-phosphotransferase VIII
                     /note="selection marker aminoglycoside
                     3'-phosphotransferase gene (aph) from Streptomyces rimosus"
                     /protein_id="AQN67803.1"
                     /translation="DDALRALRGRYPGCEWVVVEDGASGAGVYRLRGGGRELFVKVAAL
                     GAGVGLLGEAERLVWLAEVGIPVPRVVEGGGDERVAWLVTEAVPGRPASARWPREQRLD
                     VAVALAGLARSLHALDWERCPFDRSLAVTVPQAARAVAEGSVDLEDLDEERKGWSGERL
                     LAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLRE
                     LAHEEDPWFGPECSAAFLREYGRGWDGAVSEEKLAFYRLLDEFF"
     regulatory      7991..8230
                     /regulatory_class="terminator"
     promoter        complement(8249..8267)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8288..8304)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8312..8328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8336..8366)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8381..8402)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8690..9278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9452..10309)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10310..10414)
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 taatcgaatt cctgcagccc gggggatcca ctagttctag aaagatggaa ggggcaggac
      421 acccacgccg cagcaccgcc tgcaccagca tcccttgcgc aggtcgtgat atagcagcaa
      481 gtaagtgtaa atccaggttc cggcgaagac acgcacccag gtagaccctg gtcatgtggt
      541 cactgagctt ccctgagcct ctcgcgctca ctgagcccct ccccctcact cgcgctcagt
      601 tgcccgccca ccgtgaacac gcgcgagcca cgcgacaatg gactcgtcgt cacgcacatg
      661 ttgcaattgc cgtgttatgc cgcaccgcag tacccagggg acgcgggaag atggtgcggg
      721 gagagggcca gggcggtcgg ccagggtcga actgggggtc tgcgcgccga gacgaaaaaa
      781 atttgcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt
      841 gctatttcta gctctaaaac ccacgaagtc ccgggagaac gacagtgctc gtcggtgtaa
      901 tatacatatc actagggcgc aaaagtcgcg tccaaacaga caccggcgcc agcccgacct
      961 ttcccgcgcg ggagcgcgtt gatcccgacc cggtaagtcc catcgtggaa cagtagcccc
     1021 aaggcccctt ccatagcgca gatgttcctg ggcgctctcc agcgtgcatg cgggttctag
     1081 gtagccacgc ccacgctgcg aggctgggaa aaaggcccat tgtgacagca agccagcttt
     1141 cgcgccattg gttgccggcg acagcccagt ggtgtggcag cttgggtatg catagcttgt
     1201 tggggtacgg gtgggcaatg actcccaacc ttgcgcgcgt aggatttatg agggcatcct
     1261 gctccctgcg caaaagcatg aatttgcacc catgcaatgc gcgaggggag cagctaccca
     1321 ccgcgcgctg ccctgctgcc aggtatcaac tggattatca atcccaatac ccaggcattt
     1381 atcttgctga aaaactcgag ccatccggaa gatctggcgg ccgccaccgc ggtggagctc
     1441 cacacacctg cccgtctgcc tgacaggaag tgaacgcatg tcgagggagg cctcaccmat
     1501 cgtcacacga gccctcgtca gaaacacgtc tccgccacgc tctccctctc acggccgacc
     1561 ccgcagccct tttgcccttt cctaggccac cgacaggacc caggcgctct cagcatgcct
     1621 caacaacccg tactcgtgcc agcggtgccc ttgtgctggt gatcgcttgg aagcgcatgc
     1681 gaagacgaag gggcggagca ggcggcctgg ctgttcgaag ggctcgccgc cagttcgggt
     1741 gcctttctcc acgcgcgcct ccacacctac cgatgcgtga aggcaggcaa atgctcatgt
     1801 ttgcccgaac tcggagtcct taaaaagccg cttcttgtcg tcgttccgag acatgttagc
     1861 agatcgcagt gccacctttc ctgacgcgct cggccccata ttcggacgca attgtcattt
     1921 gtagcacaat tggagcaaat ctggcgaggc agtaggcttt taagttgcaa ggcgagagag
     1981 caaagtggga cgcggcgtga ttattggtat ttacgcgacg gcccggcgcg ttagcggccc
     2041 ttcccccagg ccagggacga ttatgtatca atattgttgc gttcgggcac tcgtgcgagg
     2101 gctcctgcgg gctggggagg gggatctggg aattggaggt acgaccgaga tggcttgctc
     2161 ggggggaggt ttcctcgccg agcaagccag ggttaggtgt tgcgctcttg actcgttgtg
     2221 cattctagga ccccactgct actcacaaca agcccatatg gccagcagcc cccccaagaa
     2281 gaagcgcaag gtcgacaaga agtactccat cggcctggac atcggcacga acagcgtggg
     2341 ctgggccgtg atcaccgacg agtacaaggt gccgtccaag aagttcaagg tgctgggcaa
     2401 caccgaccgc cacagcatca agaagaacct gatcggcgcc ctgctgttcg actcgggcga
     2461 gaccgccgag gcgacgcgcc tgaagcgcac cgcgcgccgc cgctacacgc gccgcaagaa
     2521 ccgcatctgc tacctgcagg agatcttcag caacgagatg gccaaggtgg acgactcgtt
     2581 cttccaccgc ctggaggagt ccttcctggt ggaggaggac aagaagcacg agcgccaccc
     2641 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccga cgatctacca
     2701 cctgcgcaag aagctggtgg acagcaccga caaggcggac ctgcgcctga tctacctggc
     2761 cctggcgcac atgatcaagt tccgcggcca cttcctgatc gagggcgacc tgaaccccga
     2821 caactcggac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
     2881 ggagaacccg atcaacgcct cgggcgtgga cgccaaggcg atcctgtccg cgcgcctgtc
     2941 caagagccgc cgcctggaga acctgatcgc ccagctgccc ggcgagaaga agaacggcct
     3001 gttcggcaac ctgatcgcgc tgagcctggg cctgacgccg aacttcaagt cgaacttcga
     3061 cctggccgag gacgcgaagc tgcagctgag caaggacacc tacgacgacg acctggacaa
     3121 cctgctggcc cagatcggcg accagtacgc ggacctgttc ctggccgcga agaacctgtc
     3181 ggacgccatc ctgctgtccg acatcctgcg cgtgaacacc gagatcacga aggcccccct
     3241 gtcggcgtcc atgatcaagc gctacgacga gcaccaccag gacctgaccc tgctgaaggc
     3301 gctggtgcgc cagcagctgc cggagaagta caaggagatc ttcttcgacc agtccaagaa
     3361 cggctacgcc ggctacatcg acggcggcgc cagccaggag gagttctaca agttcatcaa
     3421 gcccatcctg gagaagatgg acggcacgga ggagctgctg gtgaagctga accgcgagga
     3481 cctgctgcgc aagcagcgca ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     3541 cgagctgcac gccatcctgc gccgccagga ggacttctac ccgttcctga aggacaaccg
     3601 cgagaagatc gagaagatcc tgacgttccg catcccctac tacgtgggcc cgctggctcg
     3661 cggcaactcc cgcttcgcgt ggatgacccg caagagcgag gagaccatca cgccctggaa
     3721 cttcgaggag gtggtggaca agggcgccag cgcgcagtcg ttcatcgagc gcatgaccaa
     3781 cttcgacaag aacctgccca acgagaaggt gctgccgaag cactccctgc tgtacgagta
     3841 cttcaccgtg tacaacgagc tgacgaaggt gaagtacgtg accgagggca tgcgcaagcc
     3901 cgccttcctg agcggcgagc agaagaaggc gatcgtggac ctgctgttca agaccaaccg
     3961 caaggtgacg gtgaagcagc tgaaggagga ctacttcaag aagatcgagt gcttcgacag
     4021 cgtggagatc tcgggcgtgg aggaccgctt caacgccagc ctgggcacct accacgacct
     4081 gctgaagatc atcaaggaca aggacttcct ggacaacgag gagaacgagg acatcctgga
     4141 ggacatcgtg ctgaccctga cgctgttcga ggaccgcgag atgatcgagg agcgcctgaa
     4201 gacgtacgcc cacctgttcg acgacaaggt gatgaagcag ctgaagcgcc gccgctacac
     4261 cggctggggc cgcctgagcc gcaagctgat caacggcatc cgcgacaagc agtcgggcaa
     4321 gaccatcctg gacttcctga agtccgacgg cttcgcgaac cgcaacttca tgcagctgat
     4381 ccacgacgac tcgctgacct tcaaggagga catccagaag gcccaggtgt cgggccaggg
     4441 cgactccctg cacgagcaca tcgccaacct ggcgggctcc cccgcgatca agaagggcat
     4501 cctgcagacc gtgaaggtgg tggacgagct ggtgaaggtg atgggccgcc acaagccgga
     4561 gaacatcgtg atcgagatgg cccgcgagaa ccagaccacg cagaagggcc agaagaacag
     4621 ccgcgagcgc atgaagcgca tcgaggaggg catcaaggag ctgggctcgc agatcctgaa
     4681 ggagcacccc gtggagaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     4741 gaacggccgc gacatgtacg tggaccagga gctggacatc aaccgcctgt ccgactacga
     4801 cgtggaccac atcgtgcccc agagcttcct gaaggacgac tcgatcgaca acaaggtgct
     4861 gacccgcagc gacaagaacc gcggcaagag cgacaacgtg ccgtcggagg aggtggtgaa
     4921 gaagatgaag aactactggc gccagctgct gaacgccaag ctgatcacgc agcgcaagtt
     4981 cgacaacctg accaaggccg agcgcggcgg cctgtcggag ctggacaagg cgggcttcat
     5041 caagcgccag ctggtggaga cccgccagat cacgaagcac gtggcgcaga tcctggactc
     5101 ccgcatgaac acgaagtacg acgagaacga caagctgatc cgcgaggtga aggtgatcac
     5161 cctgaagtcc aagctggtca gcgacttccg caaggacttc cagttctaca aggtgcgcga
     5221 gatcaacaac taccaccacg cccacgacgc gtacctgaac gccgtggtgg gcaccgcgct
     5281 gatcaagaag taccccaagc tggagagcga gttcgtgtac ggcgactaca aggtgtacga
     5341 cgtgcgcaag atgatcgcca agtcggagca ggagatcggc aaggccaccg cgaagtactt
     5401 cttctactcc aacatcatga acttcttcaa gaccgagatc acgctggcca acggcgagat
     5461 ccgcaagcgc cccctgatcg agaccaacgg cgagacgggc gagatcgtgt gggacaaggg
     5521 ccgcgacttc gcgaccgtgc gcaaggtgct gagcatgccc caggtgaaca tcgtgaagaa
     5581 gaccgaggtg cagacgggcg gcttctccaa ggagagcatc ctgccgaagc gcaactcgga
     5641 caagctgatc gcccgcaaga aggactggga ccccaagaag tacggcggct tcgactcccc
     5701 gaccgtggcc tacagcgtgc tggtggtggc gaaggtggag aagggcaagt ccaagaagct
     5761 gaagagcgtg aaggagctgc tgggcatcac catcatggag cgcagctcgt tcgagaagaa
     5821 ccccatcgac ttcctggagg cgaagggcta caaggaggtg aagaaggacc tgatcatcaa
     5881 gctgccgaag tactcgctgt tcgagctgga gaacggccgc aagcgcatgc tggcctccgc
     5941 gggcgagctg cagaagggca acgagctggc cctgcccagc aagtacgtga acttcctgta
     6001 cctggcgtcc cactacgaga agctgaaggg ctcgccggag gacaacgagc agaagcagct
     6061 gttcgtggag cagcacaagc actacctgga cgagatcatc gagcagatct cggagttctc
     6121 caagcgcgtg atcctggccg acgcgaacct ggacaaggtg ctgagcgcct acaacaagca
     6181 ccgcgacaag cccatccgcg agcaggcgga gaacatcatc cacctgttca ccctgacgaa
     6241 cctgggcgcc ccggccgcgt tcaagtactt cgacaccacg atcgaccgca agcgctacac
     6301 cagcacgaag gaggtgctgg acgcgaccct gatccaccag agcatcaccg gcctgtacga
     6361 gacgcgcatc gacctgtcgc agctgggcgg cgacaccggt gtgagcaagg gcgaggagct
     6421 gttcacgggc gtggtgccca tcctggtgga gctggacggc gacgtgaacg gccacaagtt
     6481 cagcgtgtcc ggcgagggcg agggcgacgc cacctacggc aagctgaccc tgaagttcat
     6541 ctgcacgacc ggcaagctgc ccgtgccctg gcccaccctg gtgaccacct tcggctacgg
     6601 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagagcgc
     6661 catgccggag ggctacgtgc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     6721 gacccgcgcg gaggtgaagt tcgagggcga cacgctggtg aaccgcatcg agctgaaggg
     6781 catcgacttc aaggaggacg gcaacatcct gggccacaag ctggagtaca actacaacag
     6841 ccacaacgtg tacatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     6901 ccgccacaac atcgaggacg gctcggtgca gctggcggac cactaccagc agaacacccc
     6961 catcggcgac ggcccggtgc tgctgcccga caaccactac ctgtcctacc agagcgccct
     7021 gtccaaggac cccaacgaga agcgcgacca catggtgctg ctggagttcg tgaccgccgc
     7081 cggcatcacc ctgggcatgg acgagctgta caaggaattc ggcagcggcg ccaccaactt
     7141 cagcctgctg aagcaggccg gcgacgtgga ggagaacccc ggccccgacg atgcgttgcg
     7201 tgcactgcgg ggtcggtatc ccggttgtga gtgggttgtt gtggaggatg gggcctcggg
     7261 ggctggtgtt tatcggcttc ggggtggtgg gcgggagttg tttgtcaagg tggcagctct
     7321 gggggccggg gtgggcttgt tgggtgaggc tgagcggctg gtgtggttgg cggaggtggg
     7381 gattcccgta cctcgtgttg tggagggtgg tggggacgag agggtcgcct ggttggtcac
     7441 cgaagcggtt ccggggcgtc cggccagtgc gcggtggccg cgggagcagc ggctggacgt
     7501 ggcggtggcg ctcgcggggc tcgctcgttc gctgcacgcg ctggactggg agcggtgtcc
     7561 gttcgatcgc agtctcgcgg tgacggtgcc gcaggcggcc cgtgctgtcg ctgaagggag
     7621 cgtcgacttg gaggatctgg acgaggagcg gaaggggtgg tcgggggagc ggcttctcgc
     7681 cgagctggag cggactcggc ctgcggacga ggatctggcg gtttgccacg gtgacctgtg
     7741 cccggacaac gtgctgctcg accctcgtac ctgcgaggtg accgggctga tcgacgtggg
     7801 gcgggtcggc cgtgcggacc ggcactccga tctcgcgctg gtgctgcgcg agctggccca
     7861 cgaggaggac ccgtggttcg ggccggagtg ttccgcggcg ttcctgcggg agtacgggcg
     7921 cgggtgggat ggggcggtat cggaggaaaa gctggcgttt taccggctgt tggacgagtt
     7981 cttctgataa ggatccccgc tccgtgtaaa tggaggcgct cgttgatctg agccttgccc
     8041 cctgacgaac ggcggtggat ggaagatact gctctcaagt gctgaagcgg tagcttagct
     8101 ccccgtttcg tgctgatcag tctttttcaa cacgtaaaaa gcggaggagt tttgcaattt
     8161 tgttggttgt aacgatcctc cgttgatttt ggcctctttc tccatgggcg ggctgggcgt
     8221 atttgaagcg ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     8281 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8341 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8401 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8461 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8521 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8581 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8641 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8701 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8761 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8821 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8881 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8941 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9001 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9061 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9121 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9181 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9241 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9301 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9361 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9421 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9481 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9541 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9601 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9661 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9721 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9781 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9841 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9901 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9961 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10021 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10081 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10141 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10201 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10261 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10321 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10381 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
