LOCUS       62056_466        5457 bp DNA     circular SYN 03-DEC-2019
DEFINITION  Cloning vector Cam_RbsR_TAN_LacI_KSL, complete sequence.
ACCESSION   MN207957
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Rondon RE, Groseclose TM, Short AE, Wilson CJ.
  TITLE     Transcriptional programming using engineered systems of 
            transcription factors and genetic architectures
  JOURNAL   Nat Commun 10 (1), 4784 (2019)
  PUBMED    31636266
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Rondon RE, Groseclose T, Short A, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2019) Chemical and Biomolecular Engineering, 
            Georgia Institute of Technology, 950 Atlantic dr. NW, 5110E, 
            Atlanta, GA 30332, United States
REFERENCE   3  (bases 1 to 5457)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "4784"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-2019) Chemical and Biomolecular Engineering, Georgia 
            Institute of Technology, 950 Atlantic dr. NW, 5110E, Atlanta, GA 
            30332, United States"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(139..327)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(466..1545)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1546..1623)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        2324..2401
                     /label=lacI promoter
     CDS             2402..3400
                     /codon_start=1
                     /transl_table=11
                     /product="RbsR TAN"
                     /label=RbsR TAN
                     /protein_id="QFU95601.1"
                     /translation="MKPVTLYDVAEYAGVSTATVSNVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKASHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNS
                     LLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDF
                     EFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELA
                     SFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     protein_bind    3413..3434
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      3625..4169
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        4695..4797
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4798..5454
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
       61 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
      121 gggtcgttaa atagctcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
      181 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
      241 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
      301 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
      361 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
      421 acatgccgat tttagacagg aacggtacgc cagaatcctg agtcactgcc cgctttccag
      481 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      541 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
      601 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
      661 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
      721 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc
      781 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
      841 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
      901 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
      961 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     1021 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
     1081 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     1141 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     1201 gagaagattg tgcaccgccg ttttacaggc ttcgacgccg cttcgttcta ccatcgacac
     1261 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     1321 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     1381 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     1441 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacc agggaaacgg tgcttttaga
     1501 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     1561 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcacc attcgatggt
     1621 gtcgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1681 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1741 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1801 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1861 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acgtgaccgt
     1921 gtgcttctca aatgcctgag gccagtttgc tcaggctctc cccgtggagg taataattga
     1981 cgatatgatc atttattctg cctcccagag cctgataaaa acggttagcg cttcgttaat
     2041 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
     2101 atggtgcagg gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     2161 ttgggcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
     2221 gcgacgacac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     2281 ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg
     2341 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa
     2401 tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctcta ccgcgaccgt
     2461 ttccaacgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
     2521 ggcgatggcg gagctcaatt acattcccaa ccgcgtggca caacaactgg cgggcaaagc
     2581 gagccatacc attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt
     2641 gcgtggcgtt gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga
     2701 aggcgatgaa cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg
     2761 cttgctgtta ctgtgcaccg aaacgcatca accttcgcgt gaaatcatgc aacgttatcc
     2821 gacagtgcct actgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca
     2881 ggataactcg ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac
     2941 ccgtatcgcc tgtattaccg gcccgctgga taaaactccg gcgcgcctgc ggttggaagg
     3001 ttatcgggcg gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg
     3061 tgattttgaa tttaacggcg ggtttgacgc tatgcgccaa ctgctatcac atccgctgcg
     3121 tcctcaggcc gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata
     3181 tcaggcagag ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact
     3241 ggcaagcttt atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga
     3301 gctggcgatt gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt
     3361 acaacttact ccgattctga tggaacgcgg ttcggcttag gcgcaacgca attaatgtaa
     3421 gttagctcac tcattaggca ccgggatctc gaccgatgcc cttgagagcc ttcaacccag
     3481 tcagctcctt ccggtgggcg cggggcatga ctaacatgag aattacaact tatatcgtat
     3541 ggggctgact tcaggtgcta catttgaaga gataaattgc actgaaatct agaaatattt
     3601 tatctgatta ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc
     3661 tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc
     3721 tttgaaccga ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta
     3781 gccttaaccg gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg
     3841 ccagtggtgc ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg
     3901 cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
     3961 acccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca
     4021 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccgccagg ggaaacgcct
     4081 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat
     4141 gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc tcacttccct
     4201 gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag ccatttccgc
     4261 tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aatatatcct
     4321 gtatcacata ttctgctgac gcaccggtgc agcctttttt ctcctgccac atgaagcact
     4381 tcactgacac cctcatcagt gccaacatag taagccagta tacactccgc tagcgctgat
     4441 gtccggcggt gcttttgccg ttacgcacca ccccgtcagt agctgaacag gagggacagc
     4501 tgatagaaac agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag
     4561 ttggcagcat cacccgacgc actttgcgcc gaataaatac ctgtgacgga agatcacttc
     4621 gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg
     4681 cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
     4741 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg
     4801 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
     4861 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
     4921 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
     4981 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
     5041 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     5101 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     5161 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     5221 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
     5281 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
     5341 aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg cttccatgtc
     5401 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaa
//
