LOCUS       62056_461        5388 bp DNA     circular SYN 03-DEC-2019
DEFINITION  Cloning vector Cam_RbsR_TAN_CelR_KSL, complete sequence.
ACCESSION   MN207955
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Rondon RE, Groseclose TM, Short AE, Wilson CJ.
  TITLE     Transcriptional programming using engineered systems of 
            transcription factors and genetic architectures
  JOURNAL   Nat Commun 10 (1), 4784 (2019)
  PUBMED    31636266
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Rondon RE, Groseclose T, Short A, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2019) Chemical and Biomolecular Engineering, 
            Georgia Institute of Technology, 950 Atlantic dr. NW, 5110E, 
            Atlanta, GA 30332, United States
REFERENCE   3  (bases 1 to 5388)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "4784"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-2019) Chemical and Biomolecular Engineering, Georgia 
            Institute of Technology, 950 Atlantic dr. NW, 5110E, Atlanta, GA 
            30332, United States"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(139..327)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(463..1476)
                     /codon_start=1
                     /transl_table=11
                     /product="CelR KSL"
                     /label=CelR KSL
                     /protein_id="QFU95592.1"
                     /translation="MKPVTLYDVAEYAGVSKSTVSLVVNQASHVSAKTREKVEAAIKEL
                     GYVPNRAARTLVTRRTDTVALVVSENNQKLFAEPFYAGIVLGVGVALSERGFQFVLATG
                     RSGIEHERLGGYLAGQHVDGVLLLSLHRDDPLPQMLDEAGVPYVYGGRPLGVPEEQVSY
                     VDIDNIGGGRQATQRLIETGHRRIATIAGPQDMVAGVERLQGYREALLAAGMEYDETLV
                     SYGDFTYDSGVAAMRELLDRAPDVDAVFAASDLMGLAALRVLRASGRRVPEDVAVVGYD
                     DSTVAEHAEPPMTSVNQPTELMGREMARLLVDRITGETTEPVRLVLETHLMVRESG"
     promoter        complement(1477..1554)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        2255..2332
                     /label=lacI promoter
     CDS             2333..3331
                     /codon_start=1
                     /transl_table=11
                     /product="RbsR TAN"
                     /label=RbsR TAN
                     /protein_id="QFU95593.1"
                     /translation="MKPVTLYDVAEYAGVSTATVSNVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKASHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNS
                     LLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDF
                     EFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELA
                     SFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     protein_bind    3344..3365
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      3556..4100
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        4626..4728
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4729..5385
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
       61 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
      121 gggtcgttaa atagctcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
      181 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
      241 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
      301 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
      361 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
      421 acatgccgat tttagacagg aacggtacgc cagaatcctg agtcacccgg attcccgcac
      481 catcaaatgg gtctccagca ccagccgcac cggttcggtg gtctccccgg tgatccggtc
      541 cacgagcagc cgggccatct cccggcccat cagctcggtg ggctggttga cgctggtcat
      601 cggcggttcg gcgtgctcgg ctacggtcga gtcgtcgtag ccgaccaccg ccacatcctc
      661 gggcacgcgg cgtcccgaag cacgcagcac ccgcagcgcg gccagcccca tcaagtcgga
      721 ggccgcgaac acggcgtcca cgtcgggggc ccgatccagc agctcccgca tcgcggccac
      781 cccgctgtcg taggtgaagt caccgtagct caccagcgtc tcgtcgtact ccatccccgc
      841 ggcgagcagt gcttcgcgat acccctggag gcgttccaca ccagcgacca tgtcctgcgg
      901 gcccgcgatc gtagcgatcc gccggtgccc ggtctcgatc agccgctggg tggcctggcg
      961 tcccccgccg atgttgtcga tatcgacata ggacacctgt tcttcgggga cgccgagcgg
     1021 acggccgccg tagacgtacg gcaccccggc ctcgtccagc atctgcggca gcgggtcgtc
     1081 gcggtggagc gacagcagga ggaccccgtc gacgtgctgt ccggccaggt agccgcccag
     1141 ccgctcatgc tctatcccgg agcggcccgt ggccaggacg aactggaatc cccgttcgga
     1201 cagagcaacc cccacgccga gcacgatccc ggcatagaag ggttcggcga agagcttctg
     1261 gttgttttcc gacaccacca gggctacggt gtcggtacgt cgggtgacca gggtgcgggc
     1321 tgcgcggttc ggcacgtagc ccagctcttt gatcgccgct tccacttttt cccgcgtttt
     1381 cgcagaaacg tggctggcct ggttcaccac cagggaaacg gtgcttttag agacaccggc
     1441 atactctgcg acatcgtata acgttactgg tttcatattc accaccctga attgactctc
     1501 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac cattcgatgg tgtcgcggcg
     1561 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1621 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1681 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1741 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1801 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacgtgaccg tgtgcttctc
     1861 aaatgcctga ggccagtttg ctcaggctct ccccgtggag gtaataattg acgatatgat
     1921 catttattct gcctcccaga gcctgataaa aacggttagc gcttcgttaa tacagatgta
     1981 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     2041 ggcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgct
     2101 gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgaca
     2161 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     2221 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt
     2281 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
     2341 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct accgcgaccg tttccaacgt
     2401 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
     2461 ggagctcaat tacattccca accgcgtggc acaacaactg gcgggcaaag cgagccatac
     2521 cattggcatg ttgatcactg ccagtaccaa tcctttctat tcagaactgg tgcgtggcgt
     2581 tgaacgcagc tgcttcgaac gcggttatag tctcgtcctt tgcaataccg aaggcgatga
     2641 acagcggatg aatcgcaatc tggaaacgct gatgcaaaaa cgcgttgatg gcttgctgtt
     2701 actgtgcacc gaaacgcatc aaccttcgcg tgaaatcatg caacgttatc cgacagtgcc
     2761 tactgtgatg atggactggg ctccgttcga tggcgacagc gatcttattc aggataactc
     2821 gttgctgggc ggagacttag caacgcaata tctgatcgat aaaggtcata cccgtatcgc
     2881 ctgtattacc ggcccgctgg ataaaactcc ggcgcgcctg cggttggaag gttatcgggc
     2941 ggcgatgaaa cgtgcgggtc tcaacattcc tgatggctat gaagtcactg gtgattttga
     3001 atttaacggc gggtttgacg ctatgcgcca actgctatca catccgctgc gtcctcaggc
     3061 cgtctttacc ggaaatgacg ctatggctgt tggcgtttac caggcgttat atcaggcaga
     3121 gttacaggtt ccgcaggata tcgcggtgat tggctatgac gatatcgaac tggcaagctt
     3181 tatgacgcca ccattaacca ctatccacca accgaaagat gaactggggg agctggcgat
     3241 tgatgtactc atccatcgga taacccagcc gacccttcag caacaacgat tacaacttac
     3301 tccgattctg atggaacgcg gttcggctta ggcgcaacgc aattaatgta agttagctca
     3361 ctcattaggc accgggatct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     3421 tccggtgggc gcggggcatg actaacatga gaattacaac ttatatcgta tggggctgac
     3481 ttcaggtgct acatttgaag agataaattg cactgaaatc tagaaatatt ttatctgatt
     3541 aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct ctgaaaacga
     3601 aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact ctttgaaccg
     3661 aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt agccttaacc
     3721 ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct gccagtggtg
     3781 cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3841 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac
     3901 tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac
     3961 cgaaaggcag gaacaggaga gcgcacgagg gagccgccag gggaaacgcc tggtatcttt
     4021 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
     4081 gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat
     4141 cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag
     4201 tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat
     4261 attctgctga cgcaccggtg cagccttttt tctcctgcca catgaagcac ttcactgaca
     4321 ccctcatcag tgccaacata gtaagccagt atacactccg ctagcgctga tgtccggcgg
     4381 tgcttttgcc gttacgcacc accccgtcag tagctgaaca ggagggacag ctgatagaaa
     4441 cagaagccac tggagcacct caaaaacacc atcatacact aaatcagtaa gttggcagca
     4501 tcacccgacg cactttgcgc cgaataaata cctgtgacgg aagatcactt cgcagaataa
     4561 ataaatcctg gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga
     4621 gacgttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
     4681 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     4741 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     4801 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     4861 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     4921 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     4981 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     5041 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     5101 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     5161 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     5221 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     5281 atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg
     5341 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaa
//
